You are on target for selecting genetics adapted to your location. Ahh, the thrill of being on the Hunt! In the early 80's I met a grad student from SIU-Carbondale who was collecting oyster mushrooms that grew naturally around hot springs and vents in the Littlerock Arkansas area. He was looking for mushrooms that have evolved to grow year around, for possible indoor production stains. I dropped the ball by not following up to see if he published on his project.
Amazing Dude! 👊🏻Didn't think you would do it that quick. Really appreciate it. Don't think you could of replied any better. Exactly what I was looking for, Perfecto! Other things make more sense now, also much more clarity on things. Helps a tun. 🍄 ❤ 🤠👍Respect!
Its absolutely insane how quickly one can fall down a mushroom growing rabbit hole, 3 months ago I had an idea now I got 2 mono tubs running, 2 more on the way, and now im trying to learn how to do genetics befor my first flush
Im back to this video once again trying to absorb as much knowledge and you're channel has been my #1 go to. The wealth of information you share is far beyond anything ive found anywhere else. Thank you so much for taking the time to make this videos.
Same. One day I didn’t know anything about mushrooms, 3 months later I have a 4x4 grow tent, I have 6 different kinds growing, homemade liquid syringes and agar plates in the fridge and starting to dabble with slants.
I wish I discovered your channel long before now! Was recommended by a friend and this channel is a great library for novices! Thanks for your work and free information! 🧡🍄🙏
Was the most confusing part of starting, what happens beginning to end, even though i was reading and watching and learning the language, it really helps and a ton of people are curious, awesome video! I always just say awesome but im wowed and can't describe it better😂❤🎉 awesome videos.
I wish I could do it all, but I’m just a hobbyist with a closet to use so I’m pretty much just stuck with seeking quality spore syringes and going from there hoping for the best being as sterilized as I can be. Just started growing some P. Natalensis which so far looks great in 2 rye bags and 2 streaked agar plates only a few days later those are growing out mycelium really strongly and it all looks like the same type. Fingers crossed for them.
This is the complicated process of breeding. A much simpler easier way is just mashing spores together of two varietally different but same species, they naturally breed together. Serial dilution imo is the long, slow un-needed process. Mashing spores together works just fine and can be easily verifiable in the phenotypic traits
gary i’ve been working as a neurobio lab tech for around 6 months and i cannot tell you how much of an inspiration you are to keeping me curious about genetics and mycology. i want to see some crispr and plasmid work idk if you have something in the works
Hi Gary ! The spore syringes I purchased from you are doing very good in my liquid culture and grain spawn bags. very healthy looking mycelium! And many thanks for the extra Pink Oyster you added, your the best. been watching your videos for months now and have learned so much. =
Absolutely amazing video, cleared up a lot for me thanks. The stuff about breeding for particular substrates/conditions is very exciting, definitely want to experiment with alternatives to soy hulls and would be amazing if you could breed into something more sustainable. Didn't realise that taking the spores from a mono-culture repeatedly causes problems, makes a lot of sense though. Fungal diversity FTW! Cheers Gary!
When I bought neocaridina shrimp for my aquarium, they had a pretty high early mortality rate in the first generation, but they eventually adjusted to the local water parameters over generations and I almost never see dead shrimp to this day years later.
Point being, when I bought 5 they died before they successfully bred, when I bought 15 it was over the tipping point and even a single brood of 10 or so was enough to keep the line/s going
I like mushroom hunting for my target species, then getting clean cultures from those so I'm growing native local genetics. Very rewarding. Also how do you grow enoki to keep their "wild" characteristics? I can only find bottle teks...
Very good information although for educational purposes I've been curious about cross breeding different types, as in cubes with blue meanies or penis envy or something like that just as an example is it possible? Also is it true that adding a little pasteurized maple syrup to the water helps in the different stages?
It is possible. It’s pretty hard but there’s a guy on here that has crossed two different psilocybin containing mushrooms. The very first cross like it as far as I know and it’s pretty cool. Hard work I’d assume though.
Hi Gary! Thanks so much for all your helpful videos. I have just started working with fungi and have been wondering: Do you guys know a good method how to "measure" the mycelium growth? For example measure the biomass quantitatively ? Thank you so much!
thanks for watching! you can use weight/mass growth over time or if you are using agar dishes you can draw a line and measure growth rates by the hour/day/week etc.
@@FreshfromtheFarmFungi thanks for the quick reply! I have done it by drawing the line so far and am looking for a way to really measure the biomass. Thanks for the tip! The fungi absorb nutrients from the agar whilst they grow right - so I don't really understand what the point of measuring weight for growth would then be....?
I really badly want to attend an in person class on how to breed Cordycep mushrooms. It seems like a crapshoot ordering cultures online. Sometimes you pay a lot for a culture and it doesn’t produce anything. Other times you can pick one up for $10 and it outperforms all the rest. Would really be wonderful to know I’m starting out with something great every time.
So what is your take on breeding different species of mushrooms? Is this possible? Can someone isolate a spore from let’s say Pleurotus ostreatus and a spore from Pleurotus eryngii using serial dilution and cross them together to create dikaryotic mycelium consisting of each parent species to ultimately create a new species since they are both within the same genus? Some people in the community are now claiming that they are hybridizing two separate species within a single genus using nothing but isolated haploids-> forming diploids-> checking for dikaryotic clamp connections under a scope-> and then claiming the development of a new species without actually using any sort of mutagenesis or even sequencing DNA at the end to confirm their hybrid. I think this is total BS. Phenotypic crossing within the same species (crossing strains) is one thing (totally doable as you’ve laid out here) but breeding separate species and claiming new species sounds like heresay to me. Is there some place I can contact you to talk about this? I’m currently in a heated debate with some other mycologists.
Many thanks your vids are great, do you have a video about senescence and the 'generation' nomenclature? For instance, you might see a commercial strain listed as a generation-5... What does that mean? Asking in a different way, when you are breeding spores you're getting a generation-1, correct? What would you have to do to turn that into a generation-2? I've done some mushroom growing, mostly from spores, so I might be asking a very obvious question, thanks.
This is a subject of debate - there is no universal answer but my understanding is that each generation refers to the amount of transfers from the original culture/slant - as each transfer occurs there may be slight mutations or breakdowns of the mycelium leading to senescence and unrepairable degradation. The best way to control this is to keep backup stocks of the original sample, long term storage or continuous breeding with various genes. Hope that makes sense! MUSHLOVE
there is a phenomenon called di-mon pairing that could occur but it is random and no way to verify without doing genetic tests. I am not sure if cubensis will do this in grain spawn or not but it’s worth a shot right? I believe ed garrand has some good content on this as well
Personal video request (having ordered some plates and now waiting for them to get here): You just got an agar plate. Here’s what to do next. (Same concept but for LC, Spores, etc)
Hey Gary, do you have any book or course for breeding mushrooms from total beginner? I would like to stop buying spore prints and source my own genetics
About the monoculture getting weak over time, does that applies also if you're cloning a certain monoculture getting a piece of the fruit, putting in to agar and then spawn it in grains as usual?
Love your lecture What happens to honey light malt extract culture I made some stirred bar by using glue of glue gun unfortunately I for got that the glue will melt off in pressure cooker I saw that after removing from cooker the jars were still hot but glue was all over in my culture jars I filtered them I don't have microscope to test I put some shiitake powder quarter of quarter teaspoon of powder in each jar would it grow it was yesterday morning around 30 hours ago is there any chance for them PLEASE Help me out if you have any experience in handling such a mishap.
sometimes mishaps lead to discovery- it may work but I would be concerned about contamination from shiitake powder - watch the video on needle biopsy shortcut or else you can cut a piece from an agar plate and inoculate from there or another Lc or use a syringe and scrape some tissue off of a plate and inject that way.
it is choosing a morphology of the mycelium to carry on that trait - for example people prefer the rhizomorphic (“ropy”) mycelium over slower growing dispersed mycelium so they select that section and transfer to a new plate. Typically the trait will cary over and can be honed in further - hope that makes sense! 🍄❤️
Just a short question, how about this: Taking mushrooms at their peak, put it in a blender , mix it with fertilizer , put it in a spray bottle and then spray it at the right season. Tataaah !
can you make a video that goes more in depth regarding the 'consequences' from cloning a monoculture? would this be similar to continuously cloning a 'mother' cannabis plant? i.e. the genetics degrade over time (not sure if this is the right way to view it)?
it would be similar to trying to grow a plant from pollen as opposed to a seed - half the genetic material but in the case of mycelium it would grow weakly for a bit on agar or in lc and then die off
@@FreshfromtheFarmFungi if you're cloning a mushroom doesn't it have two sets of genetics already? (fruit body requires two sets of chromosomes?) i thought monoculture are two sets of chromosomes (hence the ability to fruit).. where am i misunderstanding the definition of monoculture?
How do suppliers keep single strains going for seemingly forever? If you grow new spores, the genetics are different, but if you clone, they accumulate senescence and eventually will peter out and die or become unviable, right?
@@FreshfromtheFarmFungi Do those methods mostly depend on cold storage? Do whoever stores them rely on redundancy of storing samples across multiple places to protect against losing the strain forever if a fridge of electric system fails?
So can you please answer this question might be dumb but can you inoculate one bag with two different kinds of cubies and breed that way . Or will they just find there original spores and not mate with their cousins if that makes sense
they will just compete for space and form a zone of inhibition, then once they fruit and drop spores they would breed at that point - you can’t cross two diploid mycelium that way - maybe there is another way using reagents or genetic modification but it’s not that simple - hope that helps 👍
@@FreshfromtheFarmFungi ok makes more sense now... So If the two different fruits drop spores I would need to collect both sets into one syringe they would breed into a cross strain? Thank you for the quick reply
I subculture once every 6 months or so - I am working on better methods for long term preservation and cryo storage is the golden standard but requires some hefty infrastructure. That being said, slants hold up well and may last years I just choose to keep the myeclium fresh 👍
To point things simple mycologist try to isolate single spores so they can have eukaryotic rhizomes meaning single gene. They isolate two eukaryotic rhizomes and then let them combine making a dikaryotic rhizomes which is what can fruit mushrooms. The new rhizome is now incubated and cultured and creates either a selective breed strain or a new hybrid strain like the black pearl king oysters mushrooms (plerotus ostreotus x plerotus eryngii).
I saw a technique on the shroomery boards long ago from a guy named Roger Rabbit I think. He was talking about a technique of crossing strains by colonizing a smaller jar with a haploid mycelia. The colonization rate of haploids are slow so that why the smaller jar. Anyway after the jar is fully colonized you inject it with a multi spore syringe or a multispore plate. The mycelium mate and create a new strain to which you fruit and the spores from that new cross will be your new strain. The process the haploid mycelium goes through is called anastomosis I think. Do you have any thoughts on if this works or have you heard of it?
Here it is if you don’t want to read the whole thread: Method: First I germinated a single PF Albino spore on malt agar to produce a monokaryotic culture. Dikaryotic cultures form clamp connections that are easily visible when magnified and this culture lacked these distictive clamps. I am pretty lazy and I didn't want to spend time isolating more single spores from other cubes and then do several controlled crosses and hope for the best. I was also aware of a mechanism where a monokaryotic mycelium can become a dikaryotic mycelium by nuclear migration and not just at the new growth at the point of mycelial contact. Essentially the entire monokaryotic culture becomes dikaryotic by replicating and moving nuclei in a sort of a chain reaction through the already existing mycelial network. Sooooooo.......... here is what I did. I put the monospore PF albino culture into a jar of sterile grain (grass seed in this case) and let it colonize almost completely. Every grain was covered with mycelium but it would need a few more days to get really dense. Then I made a fresh solution (adding just dry spores also worked) of normally pigmented cubensis spores (Penis Envy or Costa Rican), injected that into the nearly colonized grain and then shook the jar to mix. The idea is to deny any uncolonized substrate to the injected cubensis spores for colonization if it combined (mated) with other cubensis spores. In theory, the cubensis spores will germinate and some will combine with other nearby spores due to clumping, but most will combine with the PF Albino mycelium. Nuclear migration should then transform the entire jar of monokaryotic Albino mycelium into several strains of dikaryotic Albino X (Penis Envy or Costa Rican) Cubensis mycelium. There shouldn't be any room left for any significant amount of pure Penis Envy or Costa Rican dikaryotic mycelium to grow and later form mushrooms.
@@FreshfromtheFarmFungi so I put spores to agar and choose the strongest mycelium and make a monoculture then put that to grain? How to I get bigger flushes? Do I just test a lot of different isolated mycelium? Thank you for your time, I like your videos very much
Does a spore print contain different genes or are all the spores from a spore print the same? In other words, can I start breeding with just one spore print or would it be necessary to already have 2 different spore prints?
I was recently asked by someone, how do you know if a hybrid is poisonous? I figured if you're breeding two edible mushrooms your going to get an edible mushroom. Is this always true and if not how does one tell if the hybrid is poisonous?
Im not sure about that - I think they would only be poisonous if you hybridize poisonous mushrooms to start with - there may be ways to analyze compounds but I imagine it’s the same as vegetables and fruit trees and offspring will result in similar traits and qualities
@@FreshfromtheFarmFungi "it’s the same as vegetables and fruit trees and offspring will result in similar traits and qualities" Aren't apple hybrids nasty most of the time lol?
@@SpencerLemay … yes crossing vegetables you get something different for sure , example , jap pumpkin cross pollinated with butternut , you can get like a stripy squash looking pumpkin and the flavour isn’t good , not in my opinion. So can only imagine crossing certain mushrooms might produce the same , hard to say , I just enjoy growing what out there that we love , if it isn’t broken ,don’t change it how I see it. Depends what a persons purpose is really.
It would be isolating a mated pair - In this video isolating would be separating single spore haploids. You cannot combine cloned fruits but can combine two isolated spores. hope that makes sense! 🍄❤️
no, It may help stabilize the genetics for a bit but ultimately it is the same as transferring tissue from another media source as the mycelium is the same tissue as the fruiting body but just constructed differently. It’s the same idea as taking a cutting from a plant and rooting it - it will carry on the age of the cutting/mycelium.
can you not mate established mycelium ? this is how I do it at home. clone two different mushrooms that I like the qualities of & place those two biopsies on the same agar plate on either side of the plate. (but not touching the petri dish walls) when the mycelium expands & meets it either rejects the mate or joins together in a nice defining fully joined matrix. (shitty that I can't upload a pic here) I than isolate that mated matrix and place it onto a new petri dish, finish colonizing the plate & use it to inoculate more petri dished, sterilized grains or make a liquid culture from it. for me this is better than choosing a single spore from 72,000,000 spores that I may want to grow.
i probably can't link the article here (DOI 10.1111/j.1574-6968.2007.01027.x) but what i got from the abstract is that even spores from a senescent mushroom will carry degraded genetic/mitochondrial material, and that the expression of those damaged genes shows up more prominently in the offspring. i also read (in a different article i think) that contact-spreading of senescent genetic material can occur when hyphae from young and old mycelium connect. seems like a kind of disease or parasitic DNA. kind of scary because it's so hard to detect and isolate. makes you wonder how these organisms have survived at all in the wild for so long. i guess frequent cross-spore breeding from young strains is the only way to keep a healthy gene line, something that seems at odds with highly controlled lab and commerical breeding. kind of a bummer at face value. curious what your thoughts are on this
this is a consistency in nature and yes introducing fresh genetics is crucial. Also, it’s important to have mother cultures that are stored correctly to go back to when signs of senescence are detected- so you can keep a relatively healthy stock culture for decades it’s the replication and use of the strain over time that weakens it generally .
I'm not super duper smart. Wollygong/Wollongong is from the same family as penis envy; I wanted to/am curious to try and make Wollygong with Penis envy dino eggs(most potent penis envy strain) but idk if it's possible! Theoretically yes but is there any science that wouldn't allow them to mate?
it could work you would need to get spores from both (probably swabs since they are notorious for having lower spore loads). then you’d need to isolate a handful of monokaryons from each spore swab, then cross them and hope for the best since there is chance they won’t be compatible- but this is the gold standard way
look up our series on it - there are many papers describing techniques and a few cultivators out there successfully doing it now - look up blues best in Indiana. I have gotten sclerotia and pins but still no fruits yet
hhhhhhhhhhh my man needs to know how to breath while you are speaking you run out of breath quite few times hhhhhh thank you for the great informations ❤
i love how your giving out wisdom like its not expensive or anything... you are my absolute favorite
thanks for watching and following along! 🍄❤️
You are on target for selecting genetics adapted to your location. Ahh, the thrill of being on the Hunt!
In the early 80's I met a grad student from SIU-Carbondale who was collecting oyster mushrooms that grew naturally around hot springs and vents in the Littlerock Arkansas area. He was looking for mushrooms that have evolved to grow year around, for possible indoor production stains. I dropped the ball by not following up to see if he published on his project.
interesting!
Amazing Dude! 👊🏻Didn't think you would do it that quick. Really appreciate it. Don't think you could of replied any better. Exactly what I was looking for, Perfecto! Other things make more sense now, also much more clarity on things. Helps a tun. 🍄 ❤ 🤠👍Respect!
🍄❤️
This is really expanding my knowledge, looks like I have a lot to learn before I can control for variables as well as a professional farm. Thank you!
Underrated channel, thank you for sharing your knowledge
thanks for watching! 🍄❤️
Its absolutely insane how quickly one can fall down a mushroom growing rabbit hole, 3 months ago I had an idea now I got 2 mono tubs running, 2 more on the way, and now im trying to learn how to do genetics befor my first flush
it is a fun and never-ending endeavor 🙏🏻🍄❤️
Im back to this video once again trying to absorb as much knowledge and you're channel has been my #1 go to. The wealth of information you share is far beyond anything ive found anywhere else. Thank you so much for taking the time to make this videos.
Same. One day I didn’t know anything about mushrooms, 3 months later I have a 4x4 grow tent, I have 6 different kinds growing, homemade liquid syringes and agar plates in the fridge and starting to dabble with slants.
I wish I discovered your channel long before now! Was recommended by a friend and this channel is a great library for novices! Thanks for your work and free information! 🧡🍄🙏
Was the most confusing part of starting, what happens beginning to end, even though i was reading and watching and learning the language, it really helps and a ton of people are curious, awesome video! I always just say awesome but im wowed and can't describe it better😂❤🎉 awesome videos.
I wish I could do it all, but I’m just a hobbyist with a closet to use so I’m pretty much just stuck with seeking quality spore syringes and going from there hoping for the best being as sterilized as I can be. Just started growing some P. Natalensis which so far looks great in 2 rye bags and 2 streaked agar plates only a few days later those are growing out mycelium really strongly and it all looks like the same type. Fingers crossed for them.
This is the complicated process of breeding. A much simpler easier way is just mashing spores together of two varietally different but same species, they naturally breed together. Serial dilution imo is the long, slow un-needed process. Mashing spores together works just fine and can be easily verifiable in the phenotypic traits
I thought if you put two varieties or "strains" together one will just overtake the other which ever one is stronger
So could you mash lions mane and bears head tooth together?
gary i’ve been working as a neurobio lab tech for around 6 months and i cannot tell you how much of an inspiration you are to keeping me curious about genetics and mycology. i want to see some crispr and plasmid work idk if you have something in the works
still saving up for the right lab gear - it’s on the list but business has other priorities atm 🙏🏻
Hi Gary ! The spore syringes I purchased from you are doing very good in my liquid culture and grain spawn bags. very healthy looking mycelium! And many thanks for the extra Pink Oyster you added, your the best. been watching your videos for months now and have learned so much. =
thanks for watching! 🍄❤️
0:02 smashed the like button & I had no idea what was coming but I knew it was going to be good !
like that t-shirt you are wearing, very 1972 !!
Absolutely amazing video, cleared up a lot for me thanks. The stuff about breeding for particular substrates/conditions is very exciting, definitely want to experiment with alternatives to soy hulls and would be amazing if you could breed into something more sustainable. Didn't realise that taking the spores from a mono-culture repeatedly causes problems, makes a lot of sense though. Fungal diversity FTW! Cheers Gary!
Super informative and I LOVE that mushlove shirt!
thanks! 🍄❤️
When I bought neocaridina shrimp for my aquarium, they had a pretty high early mortality rate in the first generation, but they eventually adjusted to the local water parameters over generations and I almost never see dead shrimp to this day years later.
Point being, when I bought 5 they died before they successfully bred, when I bought 15 it was over the tipping point and even a single brood of 10 or so was enough to keep the line/s going
I like mushroom hunting for my target species, then getting clean cultures from those so I'm growing native local genetics. Very rewarding. Also how do you grow enoki to keep their "wild" characteristics? I can only find bottle teks...
this is a great way to keep lots of diversity - I usually top fruit enoki in bags you can try that way
This is a great video! It has help understand basics that i find hard to comprehend
Thank for sharing, can you recommend some book about cultivating mushroom.
ruclips.net/video/cnFeB8yKytA/видео.html
@@FreshfromtheFarmFungi can you please tell me if we can do cross breeding
Coolest guy named Gary I know!
Love your work man! Much appreciated!
thanks for watching! 🍄❤️
3:46 Instructions unclear: the officers are demanding to know why I was running around naked, waving mushrooms around.
I appreciate all the details you include in your teaching videos ❤#MushroomFantasies
Very good information although for educational purposes I've been curious about cross breeding different types, as in cubes with blue meanies or penis envy or something like that just as an example is it possible?
Also is it true that adding a little pasteurized maple syrup to the water helps in the different stages?
It is possible. It’s pretty hard but there’s a guy on here that has crossed two different psilocybin containing mushrooms. The very first cross like it as far as I know and it’s pretty cool. Hard work I’d assume though.
bro dont forget to breathe bro, i was worried about you midway through
I do these in one take - thanks for the algorithm boost ❤️🍄
Yeah read dark psychology much ?
Hi Gary! Thanks so much for all your helpful videos. I have just started working with fungi and have been wondering:
Do you guys know a good method how to "measure" the mycelium growth? For example measure the biomass quantitatively ? Thank you so much!
thanks for watching! you can use weight/mass growth over time or if you are using agar dishes you can draw a line and measure growth rates by the hour/day/week etc.
@@FreshfromtheFarmFungi thanks for the quick reply! I have done it by drawing the line so far and am looking for a way to really measure the biomass. Thanks for the tip! The fungi absorb nutrients from the agar whilst they grow right - so I don't really understand what the point of measuring weight for growth would then be....?
I really badly want to attend an in person class on how to breed Cordycep mushrooms. It seems like a crapshoot ordering cultures online. Sometimes you pay a lot for a culture and it doesn’t produce anything. Other times you can pick one up for $10 and it outperforms all the rest. Would really be wonderful to know I’m starting out with something great every time.
So what is your take on breeding different species of mushrooms? Is this possible? Can someone isolate a spore from let’s say Pleurotus ostreatus and a spore from Pleurotus eryngii using serial dilution and cross them together to create dikaryotic mycelium consisting of each parent species to ultimately create a new species since they are both within the same genus? Some people in the community are now claiming that they are hybridizing two separate species within a single genus using nothing but isolated haploids-> forming diploids-> checking for dikaryotic clamp connections under a scope-> and then claiming the development of a new species without actually using any sort of mutagenesis or even sequencing DNA at the end to confirm their hybrid. I think this is total BS. Phenotypic crossing within the same species (crossing strains) is one thing (totally doable as you’ve laid out here) but breeding separate species and claiming new species sounds like heresay to me. Is there some place I can contact you to talk about this? I’m currently in a heated debate with some other mycologists.
Great video! I need more layman's terms when im learning anything.
thanks for watching! 🍄❤️
Great lecture dude, much appreciated
Many thanks your vids are great, do you have a video about senescence and the 'generation' nomenclature?
For instance, you might see a commercial strain listed as a generation-5... What does that mean?
Asking in a different way, when you are breeding spores you're getting a generation-1, correct? What would you have to do to turn that into a generation-2?
I've done some mushroom growing, mostly from spores, so I might be asking a very obvious question, thanks.
This is a subject of debate - there is no universal answer but my understanding is that each generation refers to the amount of transfers from the original culture/slant - as each transfer occurs there may be slight mutations or breakdowns of the mycelium leading to senescence and unrepairable degradation. The best way to control this is to keep backup stocks of the original sample, long term storage or continuous breeding with various genes. Hope that makes sense! MUSHLOVE
That is such a great channel. Thank you.
thanks for watching and following along!
I’m struggling with condensation in my agar plates. With the slightest temp change. Any tips on preventing that?
watch this short video it will help ruclips.net/video/R4BQTRrGMdU/видео.htmlsi=pT7GR7iZKmyOrv4b
Lets say i inject GT spores into a JediMF grain spawn. Would that form into a hybrid, grow both types of mushrooms, or fail and grow none?
there is a phenomenon called di-mon pairing that could occur but it is random and no way to verify without doing genetic tests. I am not sure if cubensis will do this in grain spawn or not but it’s worth a shot right? I believe ed garrand has some good content on this as well
Personal video request (having ordered some plates and now waiting for them to get here):
You just got an agar plate. Here’s what to do next.
(Same concept but for LC, Spores, etc)
Hey Gary, do you have any book or course for breeding mushrooms from total beginner? I would like to stop buying spore prints and source my own genetics
not yet Im working on one but it will be a while
Great job helped me.
Can i cross wood lover and dung lover?
it may not be this simple but I believe anything is possible
Can you make a video on your process of how to pasteurized substrate in bulk? Maybe a cheaper barrel steamer or horse trough steamer diy option
I currently use a bubba’s barrel - they are super easy to operate and I highly recommend
Amazing as always
Thank you for this video! Are there any books that cover this in extreme depth?
Im working on one
Be prepared but this book is very detailed: Genetics and Breeding of Edible Mushrooms
Can you cross breed lion's mane with bears head tooth or coral tooth?
So detailed love it
About the monoculture getting weak over time, does that applies also if you're cloning a certain monoculture getting a piece of the fruit, putting in to agar and then spawn it in grains as usual?
Love your lecture
What happens to honey light malt extract culture I made some stirred bar by using glue of glue gun unfortunately I for got that the glue will melt off in pressure cooker I saw that after removing from cooker the jars were still hot but glue was all over in my culture jars I filtered them I don't have microscope to test
I put some shiitake powder quarter of quarter teaspoon of powder in each jar would it grow it was yesterday morning around 30 hours ago is there any chance for them PLEASE Help me out if you have any experience in handling such a mishap.
sometimes mishaps lead to discovery- it may work but I would be concerned about contamination from shiitake powder - watch the video on needle biopsy shortcut or else you can cut a piece from an agar plate and inoculate from there or another Lc or use a syringe and scrape some tissue off of a plate and inject that way.
What's your weapon of choice for viewing spores? Can you get away with a stereo microscope or a compound microscope is better suited?
Is sectoring basically picking a family tribe?
it is choosing a morphology of the mycelium to carry on that trait - for example people prefer the rhizomorphic (“ropy”) mycelium over slower growing dispersed mycelium so they select that section and transfer to a new plate. Typically the trait will cary over and can be honed in further - hope that makes sense! 🍄❤️
Great video thanks! What kind of consequences will i get by breeding from a monoculture and how long do they take to manifest?
it’s a case by case scenario - every generation may be more prone to diseases or random mutations
sir, plz make some video of protoplast culture....
Sorry if I just missed it in the video, but what is the possibility of crossing haploids from different species? Say cubensis and azurescens, etc.?
some say it’s possible. I believe it can be done but I have not verified or found a verifiable source so right now it’s myco-myth
Just a short question, how about this:
Taking mushrooms at their peak, put it in a blender ,
mix it with fertilizer , put it in a spray bottle and then spray it at the right season. Tataaah !
🤣😂 it all makes sense but that’s a boatload of information!
So did I hear you say it’s possible to clone the Cordyceps? Why wouldn’t we just do that?
yes you can but they senesce very easily watch this video : ruclips.net/video/1EAewscroPc/видео.htmlsi=ZJPXYLg-DSutn2E6
Great video!
Thank you very much! SO say you've achieved a mushroom you absolutely love. You can't keep its genetics forever...?
there are ways to keep it for very extended periods yes - slants, cryo-storage are the golden standards
But eventually you'll have to part with it...? So no mushroom is identical to one that existed 10 years ago...? Thanks again.
can you make a video that goes more in depth regarding the 'consequences' from cloning a monoculture? would this be similar to continuously cloning a 'mother' cannabis plant? i.e. the genetics degrade over time (not sure if this is the right way to view it)?
it would be similar to trying to grow a plant from pollen as opposed to a seed - half the genetic material but in the case of mycelium it would grow weakly for a bit on agar or in lc and then die off
@@FreshfromtheFarmFungi if you're cloning a mushroom doesn't it have two sets of genetics already? (fruit body requires two sets of chromosomes?) i thought monoculture are two sets of chromosomes (hence the ability to fruit).. where am i misunderstanding the definition of monoculture?
So wit hthe right lab equip you can isolate spores but even then it's incredible difficult to get two different strains to breed?
yes not really difficult but just unlikely
This is awesome
does it make a difference if you take spores from the first or subsequent flush?
the second flush may be cleaner
How do suppliers keep single strains going for seemingly forever? If you grow new spores, the genetics are different, but if you clone, they accumulate senescence and eventually will peter out and die or become unviable, right?
yes they keep mother cultures in long term storage - there are a few ways to do it
@@FreshfromtheFarmFungi Do those methods mostly depend on cold storage? Do whoever stores them rely on redundancy of storing samples across multiple places to protect against losing the strain forever if a fridge of electric system fails?
So can you please answer this question might be dumb but can you inoculate one bag with two different kinds of cubies and breed that way . Or will they just find there original spores and not mate with their cousins if that makes sense
they will just compete for space and form a zone of inhibition, then once they fruit and drop spores they would breed at that point - you can’t cross two diploid mycelium that way - maybe there is another way using reagents or genetic modification but it’s not that simple - hope that helps 👍
@@FreshfromtheFarmFungi ok makes more sense now... So If the two different fruits drop spores I would need to collect both sets into one syringe they would breed into a cross strain? Thank you for the quick reply
Nice video buddy. Good going
Great vid keep it up!
Mush Love yall
thanks for watching! 🍄❤️
THANK YOU!!!
Once I've isolated a axenic culture and stored it in a slant... How often should I subculture to maintain the strain? Thanks pal
by the slim chance that you actually read this... do you manage a forum or blog anywhere? Would appreciate an invite! I'm looking for good resource
Would love a video on subculturing!
I subculture once every 6
months or so - I am working on better methods for long term
preservation and cryo storage is the golden standard but requires some hefty infrastructure. That being said, slants hold up well and may last years I just
choose to keep the myeclium fresh 👍
@@FreshfromtheFarmFungi Thanks again!
Thanks!
To point things simple mycologist try to isolate single spores so they can have eukaryotic rhizomes meaning single gene. They isolate two eukaryotic rhizomes and then let them combine making a dikaryotic rhizomes which is what can fruit mushrooms. The new rhizome is now incubated and cultured and creates either a selective breed strain or a new hybrid strain like the black pearl king oysters mushrooms (plerotus ostreotus x plerotus eryngii).
yes - mycelium and “hyphae” instead of “rhizomes” though 👍
I saw a technique on the shroomery boards long ago from a guy named Roger Rabbit I think. He was talking about a technique of crossing strains by colonizing a smaller jar with a haploid mycelia. The colonization rate of haploids are slow so that why the smaller jar. Anyway after the jar is fully colonized you inject it with a multi spore syringe or a multispore plate. The mycelium mate and create a new strain to which you fruit and the spores from that new cross will be your new strain. The process the haploid mycelium goes through is called anastomosis I think. Do you have any thoughts on if this works or have you heard of it?
yes watch the video on di-mon mating it will work for some species
Here it is if you don’t want to read the whole thread:
Method:
First I germinated a single PF Albino spore on malt agar to produce a monokaryotic culture. Dikaryotic cultures form clamp connections that are easily visible when magnified and this culture lacked these distictive clamps.
I am pretty lazy and I didn't want to spend time isolating more single spores from other cubes and then do several controlled crosses and hope for the best. I was also aware of a mechanism where a monokaryotic mycelium can become a dikaryotic mycelium by nuclear migration and not just at the new growth at the point of mycelial contact. Essentially the entire monokaryotic culture becomes dikaryotic by replicating and moving nuclei in a sort of a chain reaction through the already existing mycelial network. Sooooooo.......... here is what I did.
I put the monospore PF albino culture into a jar of sterile grain (grass seed in this case) and let it colonize almost completely. Every grain was covered with mycelium but it would need a few more days to get really dense. Then I made a fresh solution (adding just dry spores also worked) of normally pigmented cubensis spores (Penis Envy or Costa Rican), injected that into the nearly colonized grain and then shook the jar to mix. The idea is to deny any uncolonized substrate to the injected cubensis spores for colonization if it combined (mated) with other cubensis spores. In theory, the cubensis spores will germinate and some will combine with other nearby spores due to clumping, but most will combine with the PF Albino mycelium. Nuclear migration should then transform the entire jar of monokaryotic Albino mycelium into several strains of dikaryotic Albino X (Penis Envy or Costa Rican) Cubensis mycelium. There shouldn't be any room left for any significant amount of pure Penis Envy or Costa Rican dikaryotic mycelium to grow and later form mushrooms.
I mean it’s Game of Thrones with spores.
I even have a Direwolf at home.
How do you breed lions mane for larger flushes? Do you clone or start from spores?
spores or clone from the wild
@@FreshfromtheFarmFungi so I put spores to agar and choose the strongest mycelium and make a monoculture then put that to grain? How to I get bigger flushes? Do I just test a lot of different isolated mycelium? Thank you for your time, I like your videos very much
Nice brotha
Does a spore print contain different genes or are all the spores from a spore print the same?
In other words, can I start breeding with just one spore print or would it be necessary to already have 2 different spore prints?
oh, you answered that at minute 13 of the video. In that case it would be better to just clone the first generation and not do any breeding at all?
you can get stronger genetics by breeding but more more consistent results by cloning so it depends 🙂
@@FreshfromtheFarmFungi thanks! ☺️
Where do you buy your petri dishes from?
wherever is cheapest - I like hardy diagnostics, cell treat, falcons - anything sterile works 👍
Where did you get that shirt? I want one
they are on our ETSY! 🍄❤️ www.etsy.com/listing/1074537461/
Thanks Merry Christmas! Happy New Year!
Díky!
Is it like animals/humans right? If they breed in family they are going to become weaker and have problems.
What do you mean I can't just dump a bunch of spores syringes into a single LC
you can but you may end up with contamination
dam good vid.... rite to the point.
thanks for watching! 🍄❤️
And here I thought it was gonna be easy LOL.
I was recently asked by someone, how do you know if a hybrid is poisonous? I figured if you're breeding two edible mushrooms your going to get an edible mushroom. Is this always true and if not how does one tell if the hybrid is poisonous?
Im not sure about that - I think they would only be poisonous if you hybridize poisonous mushrooms to start with - there may be ways to analyze compounds but I imagine it’s the same as vegetables and fruit trees and offspring will result in similar traits and qualities
@@FreshfromtheFarmFungi Ok thank Gary, that's what I figured. U da best, love the content, keep it up! Mush Love
@@FreshfromtheFarmFungi "it’s the same as vegetables and fruit trees and offspring will result in similar traits and qualities"
Aren't apple hybrids nasty most of the time lol?
@@SpencerLemay … yes crossing vegetables you get something different for sure , example , jap pumpkin cross pollinated with butternut , you can get like a stripy squash looking pumpkin and the flavour isn’t good , not in my opinion. So can only imagine crossing certain mushrooms might produce the same , hard to say , I just enjoy growing what out there that we love , if it isn’t broken ,don’t change it how I see it. Depends what a persons purpose is really.
Your name is gary too!
I wish it is possible to cross breed mushroom with trich
filamentous fungi and molds have very different genetics but that doesn’t mean it’s impossible
If i clone a fruit is that considered isolating?
Great vídeos btw 😘
It would be isolating a mated pair - In this video isolating would be separating single spore haploids. You cannot combine cloned fruits but can combine two isolated spores. hope that makes sense! 🍄❤️
@@FreshfromtheFarmFungi got a little reading about this :) Separating spores looks way simpler than using snake venom 😂
Does cloning a fruit body restart the generations
no, It may help stabilize the genetics for a bit but ultimately it is the same as transferring tissue from another media source as the mycelium is the same tissue as the fruiting body but just constructed differently. It’s the same idea as taking a cutting from a plant and rooting it - it will carry on the age of the cutting/mycelium.
Ah ok cool
can you not mate established mycelium ?
this is how I do it at home.
clone two different mushrooms that I like the qualities of & place those two biopsies on the same agar plate on either side of the plate. (but not touching the petri dish walls)
when the mycelium expands & meets it either rejects the mate or joins together in a nice defining fully joined matrix.
(shitty that I can't upload a pic here)
I than isolate that mated matrix and place it onto a new petri dish, finish colonizing the plate & use it to inoculate more petri dished, sterilized grains or make a liquid culture from it.
for me this is better than choosing a single spore from 72,000,000 spores that I may want to grow.
In general, this method would not result in a new genotype besides a few exceptions of species that do spontaneously mate like that.
i probably can't link the article here (DOI 10.1111/j.1574-6968.2007.01027.x) but what i got from the abstract is that even spores from a senescent mushroom will carry degraded genetic/mitochondrial material, and that the expression of those damaged genes shows up more prominently in the offspring. i also read (in a different article i think) that contact-spreading of senescent genetic material can occur when hyphae from young and old mycelium connect. seems like a kind of disease or parasitic DNA. kind of scary because it's so hard to detect and isolate. makes you wonder how these organisms have survived at all in the wild for so long. i guess frequent cross-spore breeding from young strains is the only way to keep a healthy gene line, something that seems at odds with highly controlled lab and commerical breeding. kind of a bummer at face value. curious what your thoughts are on this
this is a consistency in nature and yes introducing fresh genetics is crucial. Also, it’s important to have mother cultures that are stored correctly to go back to when signs of senescence are detected- so you can keep a relatively healthy stock culture for decades it’s the replication and use of the strain over time that weakens it generally .
Four word comment entered :)
Do you have a discord ?
no we had been on a few forums but it became too much
I'm not super duper smart. Wollygong/Wollongong is from the same family as penis envy; I wanted to/am curious to try and make Wollygong with Penis envy dino eggs(most potent penis envy strain) but idk if it's possible! Theoretically yes but is there any science that wouldn't allow them to mate?
it could work you would need to get spores from both (probably swabs since they are notorious for having lower spore loads). then you’d need to isolate a handful of monokaryons from each spore swab, then cross them and hope for the best since there is chance they won’t be compatible- but this is the gold standard way
@@FreshfromtheFarmFungiAwesome advice and great response time! Thank you and Mush Love 😆🍄🩵
Mush cult's saying you can cultivate morels and pointed us this direction. ....did I take newb-bait?
look up our series on it - there are many papers describing techniques and a
few cultivators out there successfully doing it now - look up blues best in Indiana. I have gotten sclerotia and pins but still no fruits yet
are you aware of di-mon mating?
if not, check out the buller phenomenon
cool thanks for this info - going down a new rabbit hole of complexity this morning 👍
of course. thank you for your content as well. your videos are high quality and you are well-spoken.
the rabbithole never ends.
hhhhhhhhhhh my man needs to know how to breath while you are speaking you run out of breath quite few times hhhhhh
thank you for the great informations ❤
I do these vids in one take often between inoculations - iykyk hah
So hard to watch this!
no one is forcing you 😆 I am a work in progress my friend
There is Nothing "simplified" about this video.
I don't think you could make something so simple sound more complicated if you tried.
it is how my brain organizes this. Some people may explain better for others
should learn how to breath with your nose, their is a lot of benefit.
thanks for the algorithm boost
agger ahahha. AG-ARE