thanks for the clear explanation about enzyme assays. i've been trying to find an explanation without having to read 50 pages about all types of assays.
Just wanted to say thank you for making this video, and explaining concepts the way you do. :) I've just started my degree in biochem, kind of been struggling in my first term because my mind is a mess! The way this video is structured however just made me focus in a way I haven't been able to for a while. I think it's the fact you started out with the real-life applications of the thing being studied, which is often not done in textbooks in the very beginning making it difficult (for me at least) to engage in the theory. The notes work well, it acts as a visual aid to where I am in the theory and I appreciate how you repeat each key point a final time - it's as if you know my mind would wander off and then by the time I become aware of this and alert you finalise the point I should've been paying attention to, but you don't overdo it as though to appear patronising. Overall, a very balanced video (in terms of different methods of learning), really taught me some things on the way I best learn too. Keep doing what you're doing!
this is great, thanks alot 👌 a much better explenation than any of my schoolteachers. They use to over complicate things. This is a verry clear and well spoken lecture
You are such a blessing. I was excited to see that you have a video on this topic i needed clarity about because I know you would do absolute justice to it and you didn't disappoint. Thanks for all you put into this. God bless you.
thank you very much for your videos. you have help me a lot in genetic, enzymology, biochemistry and physic domain to pass my exams. Thanks a lot l really enjoy your videos
Hi. This video is great! I only have one commentary about it: the enzyme activity can actually be defined in many other ways, since it can only be measured indirectly, the units and the definition of the activity scale depends on the experiment that takes place in order to obtain such value. I think it's important to reinforce this to prevent anyone to believing this is the only possibility.
Thanks for your best video , its really helpful just to say if you could make one more video about how to calculate other related things such as Total protein, Total activity, Yield, Specific activity and purification factor . thanks again
Hello Everyone, please I have a question regarding the calculation of relative activity of enzyme during regular intervals of Time over a long time period ( 3 weeks for example). I am working on the immobilization of cellulase enzyme for cellulose hydrolysis and I would like to estimate thé relative activity of immobilized cellulase over 1, 2 and 3 weeks. As the activity of cellulase is defined as the amount of glucose produced per minutes, its activity over a long Time ( 1, 2 or 3 weeks) will be significantly affected by the incubation Time. Then I would like to ask how Can i calculate this rekative activity taking in consideration the reaction Time ( in minutes).
How do you find out the moles of product? Lets say we have various conecentrations of an enzyme and want to figure out the no. of moles of each sample. How would you do that?
I would like to thank you for the clarification. can you help me . plz ? I want to know How do I test the lipase enzyme activity in the lab? (Laboratory experience for their immobilization lipase process
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thanks for the clear explanation about enzyme assays. i've been trying to find an explanation without having to read 50 pages about all types of assays.
itogisch lol :-) you're welcome
Just wanted to say thank you for making this video, and explaining concepts the way you do. :) I've just started my degree in biochem, kind of been struggling in my first term because my mind is a mess! The way this video is structured however just made me focus in a way I haven't been able to for a while. I think it's the fact you started out with the real-life applications of the thing being studied, which is often not done in textbooks in the very beginning making it difficult (for me at least) to engage in the theory. The notes work well, it acts as a visual aid to where I am in the theory and I appreciate how you repeat each key point a final time - it's as if you know my mind would wander off and then by the time I become aware of this and alert you finalise the point I should've been paying attention to, but you don't overdo it as though to appear patronising. Overall, a very balanced video (in terms of different methods of learning), really taught me some things on the way I best learn too. Keep doing what you're doing!
Same here..
Just started with the course n the videos helped a lot
this is great, thanks alot 👌 a much better explenation than any of my schoolteachers. They use to over complicate things. This is a verry clear and well spoken lecture
Thank you! I think I will be watching your video's for the entire weekend before my biochemistry exam :-)
That literally me rn 😂 4 years later and this hold true
@@kibanaru221 I passed so good luck!
You are such a blessing. I was excited to see that you have a video on this topic i needed clarity about because I know you would do absolute justice to it and you didn't disappoint. Thanks for all you put into this. God bless you.
great explanation! I'm taking biocehm and your videos are helping a lot.
I honestly couldn't understand my lecterurs notes so by watching this video it does really helpful. Thank you so much!
thank you very much.....i am confuse between enzyme activity and specific activity
after showing this all doubts goes into dustbin...good
thank you very much for your videos. you have help me a lot in genetic, enzymology, biochemistry and physic domain to pass my exams. Thanks a lot l really enjoy your videos
YOU ARE MY FAV PERSON IN THE WORLD
7:05 screen shot
lol nice of you but i seen ur comment after having to screenshot twice real quick lol
Thank u brother 😂 🥰
Hi. This video is great! I only have one commentary about it: the enzyme activity can actually be defined in many other ways, since it can only be measured indirectly, the units and the definition of the activity scale depends on the experiment that takes place in order to obtain such value. I think it's important to reinforce this to prevent anyone to believing this is the only possibility.
Thank you so much for the clarification.
Really appreciate your efforts.
Best regards
You're an excellent teacher! Thank you so much!
🤓
Thank you so much for explaining this in simplified way 👏I was trying to understand this topic from books, google but ended up getting more confused.
Quite a super tutor👍
Thanks for your best video , its really helpful just to say if you could make one more video about how to calculate other related things such as Total protein, Total activity, Yield, Specific activity and purification factor . thanks again
Thank you very much, sir. You've helped me a lot in many, many ways.
what is the sample used for enzyme assay
Easiest lectures, thank you!!
Thanks 😊😘 for uploading video on this topic
You're amazing.
You're the best I wish you did a CARS series!
Helpful video
Excellent, well done!
this helped. thank you.
Well explained
excellent, thank you very much!
is specific activity the enzyme activity divided by the amount of total enzyme.... or the activity divided by the amount of that specific enzyme?
What assay would you recomend for protease activity with microgram concentration?
Hello Everyone, please I have a question regarding the calculation of relative activity of enzyme during regular intervals of Time over a long time period ( 3 weeks for example). I am working on the immobilization of cellulase enzyme for cellulose hydrolysis and I would like to estimate thé relative activity of immobilized cellulase over 1, 2 and 3 weeks. As the activity of cellulase is defined as the amount of glucose produced per minutes, its activity over a long Time ( 1, 2 or 3 weeks) will be significantly affected by the incubation Time. Then I would like to ask how Can i calculate this rekative activity taking in consideration the reaction Time ( in minutes).
How do you find out the moles of product? Lets say we have various conecentrations of an enzyme and want to figure out the no. of moles of each sample. How would you do that?
My question exactly.
I would like to thank you for the clarification. can you help me . plz ? I want to know How do I test the lipase enzyme activity in the lab? (Laboratory experience for their immobilization lipase process
What does the enzyme that has high absorabnce with lower rate of change mean?
thank you so much !
How to calculate the enzyme concentration?
why can you not use specific activity when high purity is required
Hello. How to turn activity 1: 10000 to U / mg? Thank you very much.
Excellently described Sir
I loved every vedio... But this vedio was just above my mind... I was unable to understand it
How does one calculate percentage recovery?
How to calculate the concentration
thank youuu
not all heros where capesthanks dude:)
Is there anything this channel doesn't offers ???
Write the words in big, because I can't understanding,it's so small wards
Survived online classes
Thank you so much!