man u make me cry . i wished u had make thisssss before . u know how much time i have spent to learn this staff . u are very teacher and u explain staff veryyyyyyy goood . thanks
I would like to ask why grinding cells only break plasma membrane, how can we control such that the membranes of organelles are not broken during this process?
@Tony Lee before cell fractionation takes place, the organelles are buffered, isotonic, and placed in a cold solution. Buffering - so that the pH doesn’t change and therefore the enzyme structure/function doesn’t alter (change). Isotonic - has the same water potential inside + outside to PREVENT ORGANELLES from shrivelling/bursting. Placed in a cold solution - to reduce ENZYME ACTIVITY so that the enzymes can’t break down the organelles. Hope that helps.
@@fk2328 Thanks a lot for the explanations for each step, now I understand clearly :) 1 quick question: can we also add some protease inhibitors (cocktails) together with the cold solution to further minimize digestion?
Can you please describe and explain how cell fractionation and ultracentrifugation can be used to isolate mitochondria from a suspension of animal cell @AK lectures
man u make me cry . i wished u had make thisssss before . u know how much time i have spent to learn this staff . u are very teacher and u explain staff veryyyyyyy goood . thanks
h ak lol thanks!! :)
better teaching than my biology teacher at school
5:12 clear view of the board
Thanku sir
Bcoz of u I understand this topic otherwise I miss it thanks alot keep it up God bless you sir
You made it so easy thank you so much
You are awesome, it's the most helpful channel when it comes to proteins, thanks, thanks, thanks!!!!
glad to hear that!
Thank you so much! This was very clear and helpful.
Thank you! It is a very helpful lecture! but i have hard time seeing which one would be the next session you mentioned in the video.
+Nawras Janoudi Check out my website for a ordered playlist
+AK LECTURES (Andrey K) I think i have found a gem! your website is awesome and the videos are extremely helpful! I really appreciate your hard work.
thanks a lot. could you talk about ultracentrifugation ?
Thank you so much!
Thanks for whole your videos about biochemistry.
xcellent sir, realy enjoyed ur lecture and learnt alot
I would like to ask why grinding cells only break plasma membrane, how can we control such that the membranes of organelles are not broken during this process?
@Tony Lee
before cell fractionation takes place, the organelles are buffered, isotonic, and placed in a cold solution.
Buffering - so that the pH doesn’t change and therefore the enzyme structure/function doesn’t alter (change).
Isotonic - has the same water potential inside + outside to PREVENT ORGANELLES from shrivelling/bursting.
Placed in a cold solution - to reduce ENZYME ACTIVITY so that the enzymes can’t break down the organelles.
Hope that helps.
@@fk2328 Thanks a lot for the explanations for each step, now I understand clearly :)
1 quick question: can we also add some protease inhibitors (cocktails) together with the cold solution to further minimize digestion?
Can you please describe and explain how cell fractionation and ultracentrifugation can be used to
isolate mitochondria from a suspension of animal cell @AK lectures
Thank you for your wonderful presentation, can you please do ‘flow cytometry
How long are the tubes centrifuged for? (at 1000gs and 10000 gs)
if we want to separate the proteins, why can't we centrifuge at 100,000g's at once and get the supernatant?
Love from Pakistan 💕💯
I love you!
your the best
thanks!
ily
I can't a thing of what you're saying. you're too fast sir