Single channel recording: Fiiting mechanisms to recordings. Lucia Sivilotti, Plymouth talk 3

Thia video describes how to fit a receptor mechanism to single ion channel reordings.
Ciick 'SHOW MORE' to see refernces and more details.
The fits are done by the methid of maximum likelihood, i'e.the transition rates (rate constants) in a specified mechanism are adjusted to maximise their likelihood (the probability of seeing the observations given the mechanism)/In order to calculate the likelihood we need the distributions of the apparent open and shut times, allowing for the fact that both open times will appear to be longer than their true value when we miss short shut times (and similarly for shut times). These are known as HJC distributions (for Hawkes, Jalali & Colquhoun, who solved the missedbrief event problem).
References
Experimental papers
Burzomato, V., Beato, M., Groot-Kormelink, P., Colquhoun, D. & Sivilotti, L.G. (2004) Single-channel behavior of heteromeric alpha1beta glycine receptors: An attempt to detect a conformational change before the channel opens J.Neuroscience, 24, 10924-10940. Available at: www.onemol.org....
Remigijus Lape, David Colquhoun & Lucia Sivilotti (2008) On the nature of partial agonism in the nicotinic receptor superfamily (2008).Nature. 454, 722 - 728.
Available at: www.onemol.org....
Tests and explanations of our fitting methods
Colquhoun, D., Hatton, C., & Hawkes, A. G. ( 2003) The quality of maximum likelihood estimation of ion channel rate constants . Journal of Physiology (London) 547, 699-728
Available at: www.onemol.org....
More experimental papers can be downloaded from www.onemol.org....
More theoretical papers can be downloaded from www.onemol.org....
Descriptions of our fitting programs, with links to code: www.onemol.org....