Great video, well explained! However, I was wondering: what is the range to be considered a good R square value? (so that you know that your standard curve is good enough to use it to calculate unknown protein concentrations?) Also, regarding the displayed equation y=mx+b, does b (or m) have to have a specific value too to consider it to be a good standard curve? Thank you very much!
Thanks for all of your wonderful videos, I'm just wondering if you have any video here to measure growth curve and how to do that experiment under inducer effect if you have it already please paste the link here I could not find it myself.. best
You are most welcome. Please find the link below for the bacterial growth curve. We will upload another video about how to measure the bacterial growth curve over time as soon as possible. ruclips.net/video/U66FloGr-s0/видео.html
It's because his y-intercept is already negative, so when he isolates the x and moves y to the other side, it becomes positive. If you were making a spreadsheet and wanted this to work for future BCA assays that may have a positive y-intercept, you would indeed write the equation x=(y-intercept)/slope . He was just taking a short cut when writing it out 👍
@@BiologyLectures Respected Sir..Can you please help me out to find the other value? I have absorbance value of unknown sample for different time like 20 min.,30 min. and for 60 min. I have standard curve also for the same drug. I have used D-glucose and the process is In Vitro Reverse Iontophoresis Process. Now I'm struggling to find the other value. What I want to calculate: Extracted glucose Concentration (µM), Concentration for receptor chamber (µgm), Cumulative amount(µg), Glucose flux extracted(nmole cm−2 h−1), steady state flux, cumulative penetration for each sampling time.
Best lecture on RUclips ❤
thanks
Excellent this is what I was looking for.
Glad it was helpful!
Thank you so much for this presentation !
You are most welcome 🤗
Thank you so much, sir. It was well presented with a moderate pace and exceptional explanation.
Thank you very much for the kind words
Thank you very much for the kind words ❤️
Makes my life WAY easier thank you
Super amazing, you made it so easy
Thank you very much 😊
Dude you saved me!! Thankyou so much this was so helpful
You are most welcome.
That was very helpful, thanks a bunch
You are most welcome 🤗
In the equation, how to calculate 0.0455 and 0.0021 values. Also tell about R2 calculation too. Thank you
Thank you very much!
You are most welcome 🤗
thank you sooooo much for this..... very simple....
You are most welcome.
thanks, i want to know how we add dilution factor? as i dilute my sample then how i can know my actual unknown concentration. thank you
Excellent tutorial
Thank you! Cheers!
Excellent explanation
you saved me. thank you so much
You're welcome!
Hello! Thank you for the video... I would ask if you subtract the "blank" also from the sample absorbance.
Is it must for my plot points to be on the line?
Not necessary..... points may be above or below or on line....r value vary accordingly
This equation is used for testosterone analysis ?
Thank you🙏🌹❤
You are most welcome.
How do u calculate final absorbance?
Why the y intercept comming negative??
Thank you sir, well explained
Great video, well explained!
However, I was wondering: what is the range to be considered a good R square value? (so that you know that your standard curve is good enough to use it to calculate unknown protein concentrations?)
Also, regarding the displayed equation y=mx+b, does b (or m) have to have a specific value too to consider it to be a good standard curve?
Thank you very much!
Very well explained
You are welcome
but how to convert the value to mg/g from microl/ml.
Thanks for all of your wonderful videos, I'm just wondering if you have any video here to measure growth curve and how to do that experiment under inducer effect if you have it already please paste the link here I could not find it myself.. best
You are most welcome. Please find the link below for the bacterial growth curve. We will upload another video about how to measure the bacterial growth curve over time as soon as possible. ruclips.net/video/U66FloGr-s0/видео.html
How did you get the equation
As shown in the video, click on show equation icon
how can you find an unknown dosage using the final concentration and the absorbance?
How do I convert the concentration gotten from the graph to percentage
aoa
reading of absorbance was measured on ------ nm ??
you did not mention it
kindly explain it
thank you
You can measure the absorbance at 562 nm.
@@BiologyLectures thank you
@@biyamushtaq9616 You are most welcome.
Easy to understand
Thank you
Great!!!
You are welcome.
Did you measure two times on the same same samples ?
Yes, that is correct. That's why we have technical duplicates.
How you calculate average?
Thankyou so much bai
You are welcome
Thank you 🫶🏼
Thanks
You are welcome
thank you so much :)
You are most welcome
Thank you very very much but Iwant explaine how absorbance =0.01,0.009 at concentration zero please
How to convert mg/ml to mg/100gram... Please reply
The equation will be x = (y- intercept)/ slope.
U are doing x = ( y+intercept)/slope
Please explain if i am doing wrong
It's because his y-intercept is already negative, so when he isolates the x and moves y to the other side, it becomes positive. If you were making a spreadsheet and wanted this to work for future BCA assays that may have a positive y-intercept, you would indeed write the equation x=(y-intercept)/slope . He was just taking a short cut when writing it out 👍
Concentration sy phly wali absorbance kha sy i
How is y=mx-c
I have one doubt that the concentration of unknown will be in mg/ml or mg/100 gram?
It will be in mg/ml.
The Concentration I got from the Spectrophotometer and from this equation are totally different.
Then please follow the instructions shown in the video to calculate the concentration
How i come Germany ❤😊
You can come to study or work in Germany.
@@BiologyLectures Tell me the process please
@@BiologyLectures can you call me..?
@@BiologyLectures Respected Sir..Can you please help me out to find the other value? I have absorbance value of unknown sample for different time like 20 min.,30 min. and for 60 min. I have standard curve also for the same drug. I have used D-glucose and the process is In Vitro Reverse Iontophoresis Process. Now I'm struggling to find the other value. What I want to calculate: Extracted glucose Concentration (µM), Concentration for receptor chamber (µgm), Cumulative amount(µg), Glucose flux extracted(nmole cm−2 h−1), steady state flux, cumulative penetration for each sampling time.
Can you please share your mail i'd here..??