This was very helpful, but I have a question for you. I don't know the value "Y value is < than lower asymptote." I think the value is similar the blank value. How can I deal with it.
Hi, thanks for your comment. The lower asymptote refers to the lowest value that can be obtained from the curve (represented by either the a or d parameter, depending on the type of assay you're performing). Samples with values outside the asymptotes of the curve cannot return calculable concentration results. To allow me to assist further and answer any additional questions you may have, please send me an email to support@myassays.com. Thanks!
Hi! The ELISA spreadsheet template is a fantastic starting point for analyzing ELISA data. You can update this template to suit any plate layout or number of standards included in your assay. If you'd like a demonstration, or an updated example spreadsheet, please send us an email at support@myassays.com with additional information about your analysis requirements and we'll be happy to assist!
Hi, Thank you for your comment! Lower R² values can be indicative of a poor fit. In these instances, it is recommended to review the assay procedure and obtained data for the standards. Check the standards follow the expected trend for the assay. If everything is as expected, it may be that a different fit method should be used. To easily evaluate different fit methods, you may find our free curve fitting tools available at www.MyCurveFit.com to be helpful. If you would like additional assistance specific to your data, please send your assay and run information to support@myassays.com.
Hi Fernanda Decaro! Yes, the MyCurveFit Excel Add-In can be used to perform analysis for competitive ELISA, and the template available for download will provide a great starting point. Please email us at support@myassays.com and we will be happy to assist further with creating a spreadsheet specific to your analysis.
Hi! The MyCurveFit Excel Add-In can be used to perform analysis for a wide range of assays, to allow me to assist further with your specific application, please provide the kit insert or detailed data analysis instructions to support@myassays.com. Thanks!
Thanks . One more question please . I want to process Rubella IgM Quantitative Test, So My Elisa Plate Reader is Elisa Mindray. So which parameter is good to read the plate like (Absorbance, 4-Parametric , log to log or Point-to-point ) Thanks
Hello! Thank you for your comment. I'll be happy to assist further with your data analysis. Could you please send an email to support@myassays.com with your kit insert or data analysis instructions?
Hi, thank you for your comment. I'll be happy to provide further support via email or a brief GoToMeeting to help you get started. Please email us at support@myassays.com to allow me to assist. Thanks!
Hello, thank you for your message. To calculate the dilution factor, use the formula: Dilution Factor = (Final Volume)/(Initial Sample Volume) So for example if you've diluted a sample 1 part to 4 parts diluent, you might use 100µL sample and 400µL diluent. Using the above formula: Dilution Factor = (100µL+400µL)/(100µL) Dilution Factor = 500µL/100µL Dilution Factor = 5 If you have any additional questions, don't hesitate to reach out via email: support@myassays.com. Thanks!
Hi! Thank you for your message. To allow me to get a little additional information and assist further with troubleshooting, please email us directly at support@myassays.com. Thanks!
Hi, thank you for your interest in the MyCurveFit Excel Add-In! You can download a free trial from our website: www.myassays.com/tools/myassays-excel-add-in, or you can purchase a license directly from our online store: www.myassays.com/store.aspx If you have any additional questions please email us at support@myassays.com.
I did SIRT1 ELISA. I got the OD. But I don't know how to represent my data or what to infer from it. I want to see if my compound is a SIRT1 activator or Inhibitor. Can you help me? It'll be a great help! Thank You.
hello good afternoon my name Reguinaldo, can you help me what would be the best way to quantify my protein of interest, by capture ELISA? I can plot absorbance vs concentration or use 4P or 5P. Now how can I do it in Excel as in your video, do I need a program to obtain the formula of = mycurve4P? Thank you
Hi Reguinaldo! You can download a free trial of the MyCurveFit Excel Add-In at the following link to begin analyzing 4PL and 5PL data directly within Excel: www.myassays.com/download-and-install-mycurvefit-excel-add-in.html I will be happy to assist with creating an Excel template to analyze your data, please email support@myassays.com with your assay requirements. Thanks!
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This was helpful. You even reply when we are stuck at any step.Thank you
This was very helpful
This was very helpful, but I have a question for you. I don't know the value "Y value is < than lower asymptote." I think the value is similar the blank value. How can I deal with it.
Hi, thanks for your comment. The lower asymptote refers to the lowest value that can be obtained from the curve (represented by either the a or d parameter, depending on the type of assay you're performing). Samples with values outside the asymptotes of the curve cannot return calculable concentration results. To allow me to assist further and answer any additional questions you may have, please send me an email to support@myassays.com. Thanks!
Hi,
What if I have 10 standards for the ELISA? Is the ELISA spreadsheet template able to analyze my ELISA data?
Thanks
Hi! The ELISA spreadsheet template is a fantastic starting point for analyzing ELISA data. You can update this template to suit any plate layout or number of standards included in your assay. If you'd like a demonstration, or an updated example spreadsheet, please send us an email at support@myassays.com with additional information about your analysis requirements and we'll be happy to assist!
What happens when R2 value is 0.8819? It does not fit the curve well. Should I go for polynomial regression instead of linear?
Hi, Thank you for your comment! Lower R² values can be indicative of a poor fit. In these instances, it is recommended to review the assay procedure and obtained data for the standards. Check the standards follow the expected trend for the assay. If everything is as expected, it may be that a different fit method should be used. To easily evaluate different fit methods, you may find our free curve fitting tools available at www.MyCurveFit.com to be helpful. If you would like additional assistance specific to your data, please send your assay and run information to support@myassays.com.
This template will function for competitive Elisa? I´m dyIng trying to standarize my data
SOS
Hi Fernanda Decaro! Yes, the MyCurveFit Excel Add-In can be used to perform analysis for competitive ELISA, and the template available for download will provide a great starting point. Please email us at support@myassays.com and we will be happy to assist further with creating a spreadsheet specific to your analysis.
Hi, is this applicable to DAS-ELISA in Potato pathogen disease testing using Bioreba kits.?
Hi! The MyCurveFit Excel Add-In can be used to perform analysis for a wide range of assays, to allow me to assist further with your specific application, please provide the kit insert or detailed data analysis instructions to support@myassays.com. Thanks!
Thanks . One more question please .
I want to process Rubella IgM Quantitative Test, So My Elisa Plate Reader is Elisa Mindray. So which parameter is good to read the plate like (Absorbance, 4-Parametric , log to log or Point-to-point ) Thanks
Hello! Thank you for your comment. I'll be happy to assist further with your data analysis. Could you please send an email to support@myassays.com with your kit insert or data analysis instructions?
@@myassays Ok Thank You
Can you tell me how to bring the mycurve formula into the excel sheet. It is not showing for me.
Hi, thank you for your comment. I'll be happy to provide further support via email or a brief GoToMeeting to help you get started. Please email us at support@myassays.com to allow me to assist. Thanks!
Please who did you calculate your dilution factor
Hello, thank you for your message. To calculate the dilution factor, use the formula:
Dilution Factor = (Final Volume)/(Initial Sample Volume)
So for example if you've diluted a sample 1 part to 4 parts diluent, you might use 100µL sample and 400µL diluent. Using the above formula:
Dilution Factor = (100µL+400µL)/(100µL)
Dilution Factor = 500µL/100µL
Dilution Factor = 5
If you have any additional questions, don't hesitate to reach out via email: support@myassays.com. Thanks!
what if my standard curve is prepared from hyperimmune serum, how can I backcalculate this data to the standard dilution curve
Hi @susanlopez8531! I'll be happy to assist, could you please provide your example data and analysis requirements by email to support@myassays.com?
downloaded the add in, but it doesn't show on the tool bar after restarting. Any ideas what the issue might be?
Hi! Thank you for your message. To allow me to get a little additional information and assist further with troubleshooting, please email us directly at support@myassays.com. Thanks!
should we buy for a serial code? how to pay for it?
Hi, thank you for your interest in the MyCurveFit Excel Add-In! You can download a free trial from our website: www.myassays.com/tools/myassays-excel-add-in, or you can purchase a license directly from our online store:
www.myassays.com/store.aspx
If you have any additional questions please email us at support@myassays.com.
I did SIRT1 ELISA. I got the OD. But I don't know how to represent my data or what to infer from it. I want to see if my compound is a SIRT1 activator or Inhibitor. Can you help me? It'll be a great help! Thank You.
Hello! I'll be happy to assist, with your analysis. Please send an email to support@myassays.com to allow further discussion of your requirements!
hello good afternoon my name Reguinaldo, can you help me what would be the best way to quantify my protein of interest, by capture ELISA? I can plot absorbance vs concentration or use 4P or 5P. Now how can I do it in Excel as in your video, do I need a program to obtain the formula of = mycurve4P? Thank you
Hi Reguinaldo! You can download a free trial of the MyCurveFit Excel Add-In at the following link to begin analyzing 4PL and 5PL data directly within Excel:
www.myassays.com/download-and-install-mycurvefit-excel-add-in.html
I will be happy to assist with creating an Excel template to analyze your data, please email support@myassays.com with your assay requirements. Thanks!