Very nice presentation. A few items to add: Since the blank represents 0 concentration and 0 absorbance, you can plot that as a sixth data point (0,0). In fact, that is why the best-fit straight line (as yours appears to) should begin at the point where the axes meet. Also, I would point out that making sure the best-fit line leaves about as many data points above it as below it (as you did) ensures that this line is "averaging out" any imperfections in the measurements.
This is an absolutely fantastic video!! I thoroughly understand the concepts of the lab now and am much more confident going in tomorrow! Thank you so much you were such a huge help.
It would, but for this particular class we are simply introducing the concept of how protein concentration and absorption relate to one another, as well as how a standard curve could be constructed from that obtained data. Beyond that, many laboratories still do perform standard curves alongside protein assays, so this is intended to provide the students with the protocol associated with those assays.
Thank you so much for this video! my professor didn't answer my question about standard curves, but this video most definitely did! You are an excellent professor! thank you!
Thank you for this video! I been trying to solve this through Beer's Law formula and only lead me to deeper confusion. Plotting my points and finding the X-axis with absorbance number is much easier.
god bless you and your family, you have no idea how much help you provided for us. may the reward of this help be paradise in the afterlife and a lot of happiness with prosperity and good health in this world for you and your family. Amen
Help with a question ????? A compound absorb light in 227 nm. The standard curve calibration for the compound is Abs = 372x + 0,6432 which the concentration is g/100mL. The technique was analytical standard adding from fluoxetine hydrochloride (C17H18F3NO.HCl - molar mass = 345,79 g/mol). So determine the concentration in mol/L from the analysed solution. Show all calcs progression.
Thanks and well explained in conceptual term. I would like to ask that if possible, please show the use of spectrophotometer because new researchers faced tedious problems particularly when no Lab technicians are available.
Wouldn't it be more precise to use the beer-Lambert law equation to figure out the molar extinction coefficient of the Protien, then use that to find the unknown concentration?
If I have a standard solution of 5.0 ug/ml P and want to make dilutions of 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, and 5 mg/L to make a standard curve to determine mg/L P in a sample how would I prepare those dilutions ?
Oh my goodness. Young man, whoever you are, God bless you. This finally makes sense.
Amen
You just saved my life for my uni assessment!!!!
Happy to help!
How wonderful this was! It cleared SOOOOO many concepts up for me.
Thank you so much!!! Three years and counting and this video is still helping people. I got such a better understanding!!
I see how this relates to accuracy and precision ideas, thank you
Happy to help!
It's like simple Linear Regression, now I have idea, thank you
Very nice presentation. A few items to add: Since the blank represents 0 concentration and 0 absorbance, you can plot that as a sixth data point (0,0). In fact, that is why the best-fit straight line (as yours appears to) should begin at the point where the axes meet. Also, I would point out that making sure the best-fit line leaves about as many data points above it as below it (as you did) ensures that this line is "averaging out" any imperfections in the measurements.
This is an absolutely fantastic video!! I thoroughly understand the concepts of the lab now and am much more confident going in tomorrow! Thank you so much you were such a huge help.
It would, but for this particular class we are simply introducing the concept of how protein concentration and absorption relate to one another, as well as how a standard curve could be constructed from that obtained data. Beyond that, many laboratories still do perform standard curves alongside protein assays, so this is intended to provide the students with the protocol associated with those assays.
God bless you
You just solved my assignment
Gob bless you man for making it easy for us.
finally someone who explains it like it isnt rocket science.
Thank you so much for this video! my professor didn't answer my question about standard curves, but this video most definitely did! You are an excellent professor! thank you!
Thank u so much , I can't wait to subscribe, this video explained everything I needed to know and fill in my report
Thanks for the sub, and I hope that my videos continue to help you!
Happy to help Ali! Cheers.
Thanks alot. I've been searching for such a video for three days. It was very helpful.
Good refresher!
Thank you for this video! I been trying to solve this through Beer's Law formula and only lead me to deeper confusion. Plotting my points and finding the X-axis with absorbance number is much easier.
god bless you and your family, you have no idea how much help you provided for us. may the reward of this help be paradise in the afterlife and a lot of happiness with prosperity and good health in this world for you and your family. Amen
Great thank you sir.u are best teacher,help us lots
you my friend are a lifesaver
Thank you!, that was very helpful.
Gardenia Magnifica Happy to help!
Excellent. Thank you!
You are welcome!
thank you so much for your wonderful explanation!
Help with a question ????? A compound absorb light in 227 nm. The standard curve calibration for the compound is Abs = 372x + 0,6432 which the concentration is g/100mL. The technique was analytical standard adding from fluoxetine hydrochloride (C17H18F3NO.HCl - molar mass = 345,79 g/mol). So determine the concentration in mol/L from the analysed solution. Show all calcs progression.
Thank you .that was very helpful.
I'm glad that it helped.
Thanks and well explained in conceptual term. I would like to ask that if possible, please show the use of spectrophotometer because new researchers faced tedious problems particularly when no Lab technicians are available.
Thank u so much for making this excellent video!.
Simple and easy, good in u Mark...
Extremely helpful 😊
جزاك الله خير . جدا استفدت من شرحك
Thank you, this makes sense now!
You are welcome! I'm happy that it helped!
Wow I loved that lecture. We'll explained.
Thanks a million sir!!
It is compulsory to calibrate the spectrophotometer by adding a Blanc tubs!
Wouldn't it be more precise to use the beer-Lambert law equation to figure out the molar extinction coefficient of the Protien, then use that to find the unknown concentration?
Great explanation!
Very helpful!!
man can u explain it with a y=mx+c equation.?
Thanks sir it helped me alot
excellent video
Well explained. Well Done
thx for making this simple
You are quite welcome!
May God pay you withwhat u deserves best Sir
Thanks 🙏 Much appreciated
That was helpful thank you
Thank sir .what i want to know is if i can use either beer's law to find the unknown concentration for a given absorbance or from the graph
Thank you sir...
So helpful! Thankyou!
You are quite welcome!
Thank you so much
Thank you very very much!
Thanks a lot
Easy to understand..thanks bro
would the results be not as accurate if most of the other points were not on the line?
It's really helpful ,thankuu
life savior
If I have a standard solution of 5.0 ug/ml P and want to make dilutions of 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, and 5 mg/L to make a standard curve to determine mg/L P in a sample how would I prepare those dilutions ?
Can we convert the concentration values to absorbance values lyk how we concert transmittance to absorbance values
Very helpful.But how we want to calculate by using formula?
Thank you mark
Thank u so much sir!!! My chem Professor is no help😭😭.
Very useful thx
Which data we must put in x, and which one in y?
How did u get the absorbance?
nice sir thank u..
could you plz share videos about drop count for bacteria ???
thanks .... but in this way we use beer lambert law or not ...
Thanks!
Lace Miggins ..hey
would it not be .39 instead of 3.9
Sir if hav any artical on this plz send me
How to calculate with equation
mark, you beauty
Why is it called a standard curve when you're drawing a straight line? Couldn't you draw a curve of best fit instead of a line of best fit?
How ican do it math not curve
❤️💋
How is it a standard curve but you're drawing a line of best fit
شكراً دكتور عاشت ايدك دكتور الله يطيح حظك دكتور 🥬😂
Dude I love you. Thank you, you make everything make so much sense!
You are very welcome!
How did u get the absorbance ?