I loved teaching because I wanted to share my knowledge and help others. Comments like these make me very happy to know that I am still able to do that!
only vid about blanking technique ! this is actually very helpful . im preparing for national licensure exam thanks a lot. gonna nail it with the of youtube ! hahah thanks
hi! I know this video is pretty old but I just wanted to ask how we could manually correct the blank via computations? Hoping for a response, thank you!
when you zero the spectrophotometer, do you increase the intensity of light to compensate for the absorbance of light by the blank so now you have new transmittance . let is say you have 100 transmittance with air blank, then you put blank water and you get ,90. so when you zero now, with water do you increase the intesity by 10 to make it 100 so you know now at new 100 transmitance, you compensated for the amount of light absorbed, do you increase the intensity of light from the lamp when you zero blank. thank you for explaination .
Hi sir. In blank solution there were 3 components of equal volume. But in blank test tube we have 2 components except protein. So amount of these components in blank is more than actual solution. So do absorbance depends on volume? If volume in increasing, what will be the affect?
hi what if we need to have two blank solutions? in some cases when we are evaluating the amount of radicals that are not affected by other substances in a specific solution, we need to zero the absorbance of two seperated solutions which can't be mixed. so in these special examinations we can't mix all the things except the substance that we are trying to analyze or measure. so what should we do then?
Thank you sooo much. You perhaps cannot imagine how much you help others.
I loved teaching because I wanted to share my knowledge and help others. Comments like these make me very happy to know that I am still able to do that!
thank you. The time you devoted to preparing these videos has borne fruit .It has been very helpful .
Thank you very much for the compliment. I'm glad that it helped you to understand the concept.
After 10 years :O still helpful Thanks!!!!!!!!!!!!!!
Thank you so much ❤
only vid about blanking technique ! this is actually very helpful . im preparing for national licensure exam thanks a lot. gonna nail it with the of youtube ! hahah thanks
Thanking you after 9years..!!!
Thank you! I'm happy that you found it helpful.
hi! I know this video is pretty old but I just wanted to ask how we could manually correct the blank via computations? Hoping for a response, thank you!
awesome I was just about to ask my teacher what is just actually blank but thanks to you.
Helped A Lot
Thanks.
I'm watching your videos for my AMT exam. I wish I had your knowledge. Haha! Great teacher. Thanks for the videos! Keep posting.
This video gain my subscription,thank you😊
when you zero the spectrophotometer, do you increase the intensity of light to compensate for the absorbance of light by the blank so now you have new transmittance . let is say you have 100 transmittance with air blank, then you put blank water and you get ,90. so when you zero now, with water do you increase the intesity by 10 to make it 100 so you know now at new 100 transmitance, you compensated for the amount of light absorbed, do you increase the intensity of light from the lamp when you zero blank. thank you for explaination .
Hi Mark. This was super helpful for me. Really appreciate the video.
One of my coworkers use only reagent diluted with d.water for blanking will it still work?
YOU ARE SENT FROM HEAVEN SIR!!
wow you are so good! you make me understand it very well while my teacher makes it seem so difficult love you!
Great video. Thanks
What about calibration with a blank and a know standard ? Does this create the line of best fit on the standard curve ?
Hi sir. In blank solution there were 3 components of equal volume. But in blank test tube we have 2 components except protein. So amount of these components in blank is more than actual solution. So do absorbance depends on volume? If volume in increasing, what will be the affect?
I think the volume of components in a blank solution is according to a sample solution. I think
Thank you
great lecture thank you
hi
what if we need to have two blank solutions?
in some cases when we are evaluating the amount of radicals that are not affected by other substances in a specific solution, we need to zero the absorbance of two seperated solutions which can't be mixed.
so in these special examinations we can't mix all the things except the substance that we are trying to analyze or measure. so what should we do then?
thankuu so much sir. your point clear my confusion
Sir which spectrometer is good for Coloured sample testing
Impressive teacher! Thank you. You a amazing.
Thank you very much sir to provide this lecture
Great lecture sir
Thank you so much that was very helpful
thank you so much, it's so helpful
Thankyou 🙌
well done man
How about sample blank??
helps a lot
God bless you
Thancks alot u help me
Thanks soooooooo much
You are quite welcome!
My blank values are always in negative , why is it so ?
I think the volume of components in a blank solution is according to a sample solution. I think
Have my baby.