There is no pre-programmed calibration curve available. The method requires measuring the first 6 caffeine standards (0, 2, 4, 6, 8, and 10 mg/L). Afterward, the calibration curve is determined and stored in the instrument. Please feel free to contact your local MO for further questions. Contact details can be found at www.mt.com under "Contact Us".”
Are there machines that can measure absorbance as a function of temperature? Like say you are working with something that changes color when heated or cooled
Depending on the application we would recommend the CuveT Accessory in Combination with an UV/VIS Excellence Instrument in order to determine such thermochromic behavior. For more information or to get in direct contact please check: www.mt.com/global/en/home/perm-lp/product-organizations/ana/thermostating-uvvis-peltier-temperature-control.html
This is a typical setup you will find in array spectrophotometers. As the light passes through the diffraction grating, each wavelength is diffracted at a slightly different angle, causing the different wavelengths to separate from one another. The detector then measures the amount of light at each specific wavelength, allowing us to determine the absorption or transmission spectrum of the sample. You can find a more detailed view in this video: ruclips.net/video/XAp-5r3LxQo/видео.htmlsi=TeFagcZ18dr-eVdN&t=23
Dear Jana Zadro, AAS (atomic absorption spectroscopy) and FES (flame emission spectroscopy) are analytical techniques used to determine the concentration of elements in a sample. In both AAS and FES, a sample is atomized and the resulting atomic vapor is excited by a flame or other energy source. The excited atoms then emit light at specific wavelengths, which are characteristic of the element being analyzed. The intensity of the emitted light is measured and used to quantify the concentration of the element in the sample. UV/Vis (ultraviolet-visible spectroscopy) is a technique used to measure the absorption of light in the UV and visible regions of the spectrum. It is commonly used to quantify the concentration of chromophores in a sample, which absorb light at specific wavelengths. The absorption of light by a sample at a particular wavelength is related to the concentration of the absorbing species in the sample. The main difference between AAS/FES and UV/Vis is that AAS/FES measures the emission of light from an excited sample, while UV/Vis measures the absorption of light by a sample. Additionally, AAS/FES typically requires more specialized equipment and expertise to perform compared to UV/Vis.
Hi Servare, With the appropriate sample preparation, soil samples can be analyzed with all cuvette-based UV/VIS Excellence spectrophotometers (UV5, UV5Bio and UV7) e.g. for organic substances or inorganic ions. For more information or application support, please contact your local sales partner, contact details can be found at www.mt.com under "Contact Us".
Thank you for your question. Yes, theoretically, it is possible, because all these three organic compounds contain an aromatic ring in their chemical structure, which has strong absorbance in the UV light region and thus can be detected by a spectrophotometer. However, in practice, please note that the solubility of these organic compounds is very low. When not all is dissolved in water, the undissolved will be floating on the top of water and concentration determination is not correct. On the other hand, if the concentration is extremely low, e.g. only several ppb, the detection limit of the used spectrophotometer may affect the analysis feasibility, because the signal received by the spectrophotometer may be too weak to generate an accurate result. If these three organic compounds are mixed in water, separation is required before using spectrophotometry to determine the concentration of individual components in the mixture. For further evaluation we would higly recommend contacting your local sales representative.
loved this video! Good explanation. Thank you!
Thank you for watching!
Great video! Helped me so much in my chem assignment!
Great to hear!
really appreciate your way of explanation
Thank you so much for your kind words!
hello! if you don't mind replying asap, how do you determine the pre-programmed calibration curve? where do you get that from?
There is no pre-programmed calibration curve available. The method requires measuring the first 6 caffeine standards (0, 2, 4, 6, 8, and 10 mg/L). Afterward, the calibration curve is determined and stored in the instrument. Please feel free to contact your local MO for further questions. Contact details can be found at www.mt.com under "Contact Us".”
Thanks a lot ma'am this was an amazing explanation
Most welcome 😊
Most welcome 😊
Nice explanation and illustration. These products must be awesome.
They are!
Thank you. Good explanation. Love the clarity
Thanks for watching
Thank you very much for this great and funny explanation :)
Glad you liked it!
Thanks for making this thing look simple..really learnt a lot..tnx
Most welcome 😊
Are there machines that can measure absorbance as a function of temperature? Like say you are working with something that changes color when heated or cooled
Depending on the application we would recommend the CuveT Accessory in Combination with an UV/VIS Excellence Instrument in order to determine such thermochromic behavior. For more information or to get in direct contact please check: www.mt.com/global/en/home/perm-lp/product-organizations/ana/thermostating-uvvis-peltier-temperature-control.html
Why does the light need to be diffracted before it’s directed to the detector?? Thanks
This is a typical setup you will find in array spectrophotometers.
As the light passes through the diffraction grating, each wavelength is diffracted at a slightly different angle, causing the different wavelengths to separate from one another. The detector then measures the amount of light at each specific wavelength, allowing us to determine the absorption or transmission spectrum of the sample.
You can find a more detailed view in this video: ruclips.net/video/XAp-5r3LxQo/видео.htmlsi=TeFagcZ18dr-eVdN&t=23
Amazing explanation
Glad you liked it
Great! But what is the difference between AAS/FES and the UV/VIS? both give the concentration…
Dear Jana Zadro,
AAS (atomic absorption spectroscopy) and FES (flame emission spectroscopy) are analytical techniques used to determine the concentration of elements in a sample. In both AAS and FES, a sample is atomized and the resulting atomic vapor is excited by a flame or other energy source. The excited atoms then emit light at specific wavelengths, which are characteristic of the element being analyzed. The intensity of the emitted light is measured and used to quantify the concentration of the element in the sample.
UV/Vis (ultraviolet-visible spectroscopy) is a technique used to measure the absorption of light in the UV and visible regions of the spectrum. It is commonly used to quantify the concentration of chromophores in a sample, which absorb light at specific wavelengths. The absorption of light by a sample at a particular wavelength is related to the concentration of the absorbing species in the sample.
The main difference between AAS/FES and UV/Vis is that AAS/FES measures the emission of light from an excited sample, while UV/Vis measures the absorption of light by a sample. Additionally, AAS/FES typically requires more specialized equipment and expertise to perform compared to UV/Vis.
Which of those devices can analyze soil samples?
Hi Servare,
With the appropriate sample preparation, soil samples can be analyzed with all cuvette-based UV/VIS Excellence spectrophotometers (UV5, UV5Bio and UV7) e.g. for organic substances or inorganic ions. For more information or application support, please contact your local sales partner, contact details can be found at www.mt.com under "Contact Us".
Soilgauge
Can it determine the chlorine amount in the water?
Yes, it can. Please see this video
ruclips.net/video/fZV2O1ov8Yo/видео.html
Great video!
Thank you
nicely done, perfect Job, wish you the beset
Thank you very much!
Nice one application based knowledge here
Thanks for watching
How about the powder and thin film?
Hi,
With our solid sample holder, you may analyze thin film.
Can I measure the concentration of (Benzene , Toluene ,Ethylbenzene) in water ?
If I can do that , please tell me about the price.
with appreciate
Thank you for your question. Yes, theoretically, it is possible, because all these three organic compounds contain an aromatic ring in their chemical structure, which has strong absorbance in the UV light region and thus can be detected by a spectrophotometer. However, in practice, please note that the solubility of these organic compounds is very low. When not all is dissolved in water, the undissolved will be floating on the top of water and concentration determination is not correct. On the other hand, if the concentration is extremely low, e.g. only several ppb, the detection limit of the used spectrophotometer may affect the analysis feasibility, because the signal received by the spectrophotometer may be too weak to generate an accurate result. If these three organic compounds are mixed in water, separation is required before using spectrophotometry to determine the concentration of individual components in the mixture. For further evaluation we would higly recommend contacting your local sales representative.
Thanks
Welcome