Great vids. Thanks for the tour. It's not uncommon for Aquatic plants to "die off" when going from a submerged growing state to being grown out of the water, or when going from growing above water to being submerged, as is the case in most commercial growers. Many folks with fishtanks will worry when the nice plant they bought in the store, dies when they submerge it at home, but they will usually come back. They tend to need fairly strong lighting, as well, so your regular T5s may not be enough. Cheers.
Hey bro thanks so much for showing off your home lab… I just started my own and have been super stoked on your vids. I’ve ordered ppm excited to use it
I love your videos and have recently purchased things to start my own tissue culture at home! I have a quick question if you could please help me!! I have a small banana plant about 6 inches tall that I want to test in tissue culture, what should I use as the explant?? Can I just decapitate it and use the shoot tip?
@@PlantCellTechnology thank you for the reply!! It’s a young plant and I don’t think it will produce suckers readily yet. I was looking to culture this specific plant, maybe use the corm or preferably the shoot tip? I was trying not to kill the plant.
@@MixedUpNumberz You can. Theoretically, you can use any explant of a plant for a tissue culture process. However, you must have a working protocol for the same. Or you need to create one. Though, you may find them in a google search.
There's not one size fits for all kinds of recipes while tissue culturing plants. Every plant species has its own requirements. So, we definitely succeeded with the plants we wanted to grow in our lab and trying many other new plants. You can find the protocol for any plant you desire by searching "tissue culture of (plant's name)" in Google.
Nice I am not clear with the term biokoplar something. Can you comment here. I would also like to know is it called a submerged technique? And also how many time you need to supply media in one day to the plants
It's Biocoupler. It's the simplest form of temporary immersion bioreactor. You need to change media only when the media is exhausted and plants are outgrown. So, it's not every day. Let us know if you have any other questions.
@@kokborokbaijapan9433 Of course! Let us know if you have any other questions. And, do follow us on our social channels to stay updated on everything plant tissue culture.
hi newbie here. most of your tissue cultures are dead/disfigured due to incorrect hormone dosing? do you think it's because the media wasn't changed out? or are they supposed to survive in the media indefinitely? i read somewhere that it should be changed every few days
Hey, Good question! Of course, after a couple weeks (between 4-8, or in some cases earlier or later than this) the media is required to change. However, if the plants are dead before the arrival of its "change media time", then either the composition of the media (type and concentration of plant hormones, or any other additives in the media) is affecting the plant or the environmental conditions aren't suitable to promote the growth and development of the plant. I hope I have answered all your questions.
We do tissue culture just for teaching purposes. So, we can't give a personal estimate. However, if you do tissue culture right, grow plants, and sell it to nurseries and other plant-based companies you can earn between $1K-$4K. Nut again, that totally depends on what plants you grow, the demand for those plants in your area, how many are you growing, and your selling skills. And, if everything goes right, you can make even more than the mentioned numbers.
@@mrogers6126 you can search over the internet and talk to nurseries and ask which plants are high in demand in your area. Observe, which plants you extensively see in your neighbors' gardens or people in your city. You will be required to do a bit research.
Great vids. Thanks for the tour. It's not uncommon for Aquatic plants to "die off" when going from a submerged growing state to being grown out of the water, or when going from growing above water to being submerged, as is the case in most commercial growers. Many folks with fishtanks will worry when the nice plant they bought in the store, dies when they submerge it at home, but they will usually come back. They tend to need fairly strong lighting, as well, so your regular T5s may not be enough. Cheers.
Great video! Mind me asking what protocol you used for your jewel orchids? Many thanks!
Thanks for all of the helpful information and insight
Of course. we are here to help the tissue culture community in every way we can.
Hey bro thanks so much for showing off your home lab… I just started my own and have been super stoked on your vids. I’ve ordered ppm excited to use it
by the way, wondering if you have a discord server going?
@@almostoffthegrid Hey, amazing to hear from you!
Glad that you are enjoying our videos. Unfortunately, currently, we don't have a discord server.
I love your videos and have recently purchased things to start my own tissue culture at home! I have a quick question if you could please help me!! I have a small banana plant about 6 inches tall that I want to test in tissue culture, what should I use as the explant?? Can I just decapitate it and use the shoot tip?
Thank you so much for showing so much love to our videos.
You can use suckers of bananas as an explant.
@@PlantCellTechnology thank you for the reply!! It’s a young plant and I don’t think it will produce suckers readily yet. I was looking to culture this specific plant, maybe use the corm or preferably the shoot tip? I was trying not to kill the plant.
@@MixedUpNumberz You can. Theoretically, you can use any explant of a plant for a tissue culture process. However, you must have a working protocol for the same. Or you need to create one. Though, you may find them in a google search.
did you ever find the best media for aquatic plants?
There's not one size fits for all kinds of recipes while tissue culturing plants. Every plant species has its own requirements. So, we definitely succeeded with the plants we wanted to grow in our lab and trying many other new plants. You can find the protocol for any plant you desire by searching "tissue culture of (plant's name)" in Google.
I really appreciate your videos bud. What kind of containers are these?
These are deli containers.
Banging vid bud keep them Coming
Thanks!!
Nice I am not clear with the term biokoplar something. Can you comment here. I would also like to know is it called a submerged technique? And also how many time you need to supply media in one day to the plants
It's Biocoupler. It's the simplest form of temporary immersion bioreactor. You need to change media only when the media is exhausted and plants are outgrown. So, it's not every day.
Let us know if you have any other questions.
@@PlantCellTechnology thank you very much for the reply. Actually I want to know how many times a day you need to immersed the plants with the media
@@kokborokbaijapan9433 You only need to immerse once or twice a day for 30-60 seconds.
@@PlantCellTechnology thank you so much really very informative for me.
@@kokborokbaijapan9433 Of course! Let us know if you have any other questions.
And, do follow us on our social channels to stay updated on everything plant tissue culture.
hi newbie here. most of your tissue cultures are dead/disfigured due to incorrect hormone dosing? do you think it's because the media wasn't changed out? or are they supposed to survive in the media indefinitely? i read somewhere that it should be changed every few days
Hey,
Good question!
Of course, after a couple weeks (between 4-8, or in some cases earlier or later than this) the media is required to change.
However, if the plants are dead before the arrival of its "change media time", then either the composition of the media (type and concentration of plant hormones, or any other additives in the media) is affecting the plant or the environmental conditions aren't suitable to promote the growth and development of the plant.
I hope I have answered all your questions.
Do you mind estimating how much you make with TC? I'm disabled and no income, looking for something I can do from home & help with kids
We do tissue culture just for teaching purposes. So, we can't give a personal estimate. However, if you do tissue culture right, grow plants, and sell it to nurseries and other plant-based companies you can earn between $1K-$4K. Nut again, that totally depends on what plants you grow, the demand for those plants in your area, how many are you growing, and your selling skills. And, if everything goes right, you can make even more than the mentioned numbers.
How do I find popular plants for aquariums, ponds, and gardens in my area (google & shops)?
@@mrogers6126 you can search over the internet and talk to nurseries and ask which plants are high in demand in your area. Observe, which plants you extensively see in your neighbors' gardens or people in your city. You will be required to do a bit research.
hi bro, how to sterilized deli containers ? thanks
Hi,
You can autoclave deli containers. But, before that make sure the container is made of autoclavable plastic.
excuse me what kind of plastic container for heat resistant ?
Polypropylene (PP) and polypropylene copolymer (PPCO) made containers are autoclavable.
Ok, thank you, sir@@PlantCellTechnology
Which type Of LAF (horizontal or vertical ) is more suitable for plant tissue culture lab
Are you talking about lights?
No sir I'm talking about laminar air flow
@@ashaakshetty5313 Then, go for horizontal laminar airflow. Generally, labs prefer this one for their tissue culture procedures.
It provides better control over contamination.
Laminar air filow price
You can acquire them at prices ranging between $500-1500 (or more) based on the model, features, and the supplier of the equipment.