My daughter just killed a cactus we've had for years. I am going to attempt to clone and regrow for her mom. This video was excellent! Thank you. I have most of this stuff already, but will need the MS powder. I grow oyster mushrooms for fun, and now I'll have another little hobby.
(iam a student) usually in our lab we slowly pour the agar into the medium mixture (so that the agar dissolves easier in hot water)while we boil it cus we were instructed to do so....i never tried mixing agar with cold water....didn't think it would dissolve but u dissolved it well...and whats hard about tissue culture is maintaining a contamination free culture.
On my patreon I am doing specific media recipes for plants. Hormones are usually unique to species. I have some Basil transfers coming soon I can show the media to
Microwaves are a great way to have non sterile media and a low success rate, buy a pressure cooker for more consistent results. The microwave will work for a beginner that is not yet serious about this craft.
if you don't mind me asking, where did you learn all of this from? Do you work in a lab and this is your hobby? Thx for the great Content, super cool channel
I have worked in a lab, but this is all hobby and self taught through Google. My background is in botany and environmental science though. All ingredients can be bought for a home lab
Awesome, thanks! I love botany and work at a local botanical garden, usually I see agar solely with mycology on YT! I'll be sure to check out all ur videos. I'm impressed just self taught, I've been trying to get into agar culture, or atleast very interested! (I wish I had a little lab at home)
Sorry didn’t see this. MS you can buy with vitamins or if it’s separate I look for gamborgs vitamins. Agar I use is micropropagation agar A236 from phytotech
I have it on my site also : Check what it comes with. Some mixes have sugar, hormones, agar Agar usually most expensive. Once you understand it’s easier to make your own for cheap
Please do lots of video about tissue culture keep rocking keep shining
My daughter just killed a cactus we've had for years. I am going to attempt to clone and regrow for her mom.
This video was excellent! Thank you.
I have most of this stuff already, but will need the MS powder. I grow oyster mushrooms for fun, and now I'll have another little hobby.
Yes get the MS media with vitamins
Always graft as an option also to save cactus
Your videos are so helpful. Thank you!
Really interesting video! Thank you. Will soon join your patreon to see more details.
(iam a student) usually in our lab we slowly pour the agar into the medium mixture (so that the agar dissolves easier in hot water)while we boil it cus we were instructed to do so....i never tried mixing agar with cold water....didn't think it would dissolve but u dissolved it well...and whats hard about tissue culture is maintaining a contamination free culture.
That would be cool about the culture with hormones for other plants or cacti. Thanks for all that you do. ✌️💚🌵🌲😁
great work and great informative video
Awesome video! Thanks so much for making it 🤘
Thanks for tje knowledge 🙏
Hey David, it will be so helpful if you make a video where you explain how to do a basic media with hormones, and thank you for doing this content.
On my patreon I am doing specific media recipes for plants. Hormones are usually unique to species. I have some Basil transfers coming soon I can show the media to
Microwaves are a great way to have non sterile media and a low success rate, buy a pressure cooker for more consistent results. The microwave will work for a beginner that is not yet serious about this craft.
Good work 👍🏽
thx my gei friend
if you don't mind me asking, where did you learn all of this from? Do you work in a lab and this is your hobby?
Thx for the great Content, super cool channel
I have worked in a lab, but this is all hobby and self taught through Google. My background is in botany and environmental science though. All ingredients can be bought for a home lab
Awesome, thanks! I love botany and work at a local botanical garden, usually I see agar solely with mycology on YT! I'll be sure to check out all ur videos. I'm impressed just self taught, I've been trying to get into agar culture, or atleast very interested! (I wish I had a little lab at home)
Are there specific vitamins I should be looking for with ms powder? And is phytagel the plant agar I should be looking for?
Sorry didn’t see this. MS you can buy with vitamins or if it’s separate I look for gamborgs vitamins.
Agar I use is micropropagation agar A236 from phytotech
Sir how about auxin and cytokinin how many milliliter or ppm per liter
What ZPT you use?
I want to buy the media packets. please provide Instagram details
Do u able to put carnivorous seeds in it?
Yes 100
Link for MS Media from Amazon?
amazon's ms media is overpriced. $40 for 1 liter's worth of solution... not good at all.
I have it on my site also :
Check what it comes with. Some mixes have sugar, hormones, agar
Agar usually most expensive. Once you understand it’s easier to make your own for cheap
@@cactifanatici What's your site?
@@jakobsulzer4831 ModernPharmLA
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