This weekend will be the final setup of my home TC setup. 🎉 Pennsylvania winters are my biggest worry; last winter we had -40°F wind chill and everything in my house froze. 😕🥶 Pray for a warm winter, please! 🙏
Thanks a lot for sharing Francisco. Take care while working with flame, cleaning and tools in alcohol. I put fire inside. Also the flame risk to melt the top of the plastic container. Daniel
Have you seen any efficacy differences between cultures with saran wrap and without? The purpose would be to mostly help prevent contamination from mold spores correct?
Over the years of using the wrap, I have noticed that the media last longer, and I don't have to subculture as often. The contamination rates are also lower.
@@PlantCellTechnology thank you! I was wrapping all my cultures for a while but when I have so many cultures to do, the time can stack up and be tedious
bathrooms are wet, wet is good for molds, molds are a contam risk. probably better not in the bathroom. kitchens have a lot of bacteria so also not the best. a good procedure with stil airbox etc however allows you to work in absolute filth :D the size of the space and thus draft matters a lot in this. watch out with stil air boxes SAB using open flames and cleaning products that are flammable ;), lol okay im still watching and kudos on that demonstration . i did see a procedure mistake here, when you use a S.A.B you want to organize it in such a way that you never have to reach over your work. i seen that hand hovering over the medium going for the burner, it would be better to set it in the center ( the burner also cleans the air btw by burning off any contam, bunsenburners are sometimes used to create a clean air bubble). this hovering over things can cause things to fall from your hands or arms. so always plan out the steps and set up things so you dont have to reach over things. work front to back instead of left to right. i always imagine the center being the place of work and the sides to be the hand moving area and the back corners are storage
We didn't try orchids in our lab. But, you can easily find the protocol for the plant on the internet as it's one of the extensive tissue-cultured plants. @@Unkn0wn1133
thanks for your great work. i have 2 grams of meta toplin and need to know how to make a stock concentration and the best solvent to use can meta topolin be autoclaved? Can meta topolin concentration be stored over time?
You can learn about preparing stock solutions for plant hormones here: www.youtube.com/@PlantCellTechnology/search?query=plant%20hormone Add meta topolin in the media after it's cooled down using the syringe filter technique.
the higher % decreases the time it sits unevaporated which reduces the time its killing bacteria, it also helps getting in to the cells to have some water in it. be aware tho as alcohol isnt all that good at killing mold spores, unlike bleach solutions
I'm going to try growing some Japanese red maple seeds in TC this winter. Is the protocol different for starting seeds? I read that tree TC usually uses 1/2 MS amount but no mention of seed protocol. 🫤
Yes, generally protocols differ based on plant species and even the explant used in the process. We haven't tried the species yet in our lab. But, would love to get some update from you if you are going for it. Good luck!
@@PlantCellTechnology Thank you! I just got three cuttings from my neighbor. I know that I should take them in the spring but he wasn't sure he still be living there next spring. Will give updates. 👍💚
This is perfect timing!!! Thank you so much❤
Thank You!
This weekend will be the final setup of my home TC setup. 🎉 Pennsylvania winters are my biggest worry; last winter we had -40°F wind chill and everything in my house froze. 😕🥶 Pray for a warm winter, please! 🙏
Hi Francisco! Is the first time watching you! I am here to learn! Thanks for sharing your knowledge!!!
Hi Lily,
Hope the shared information is useful for you. Thanks for your comment.
Thanks a lot for sharing Francisco.
Take care while working with flame, cleaning and tools in alcohol.
I put fire inside.
Also the flame risk to melt the top of the plastic container.
Daniel
Thank you for the concern, Daniel!
Nice presentation!
Thank You!
Have you seen any efficacy differences between cultures with saran wrap and without? The purpose would be to mostly help prevent contamination from mold spores correct?
Over the years of using the wrap, I have noticed that the media last longer, and I don't have to subculture as often. The contamination rates are also lower.
@@PlantCellTechnology thank you! I was wrapping all my cultures for a while but when I have so many cultures to do, the time can stack up and be tedious
@@dragonsimon1 We totally get the pain.
How come the media jars you use is not hte one you sell on your shop?
bathrooms are wet, wet is good for molds, molds are a contam risk. probably better not in the bathroom. kitchens have a lot of bacteria so also not the best. a good procedure with stil airbox etc however allows you to work in absolute filth :D the size of the space and thus draft matters a lot in this. watch out with stil air boxes SAB using open flames and cleaning products that are flammable ;), lol okay im still watching and kudos on that demonstration . i did see a procedure mistake here, when you use a S.A.B you want to organize it in such a way that you never have to reach over your work. i seen that hand hovering over the medium going for the burner, it would be better to set it in the center ( the burner also cleans the air btw by burning off any contam, bunsenburners are sometimes used to create a clean air bubble). this hovering over things can cause things to fall from your hands or arms. so always plan out the steps and set up things so you dont have to reach over things. work front to back instead of left to right. i always imagine the center being the place of work and the sides to be the hand moving area and the back corners are storage
Dont u have a window in your bathroom? Mine is dry af.
You got to improvise if youre doing TC at home
wow thats cool
Your bathroom bigger than my apartment 😂😂❤
Haha!!
Do you also do flasking orchid seeds or just tissue culture plants
Currently, we are just doing tissue culturing of plants. Is there anything specific in which you are interested?
@@PlantCellTechnology orchid tissue culture?
We didn't try orchids in our lab. But, you can easily find the protocol for the plant on the internet as it's one of the extensive tissue-cultured plants. @@Unkn0wn1133
thanks for your great work. i have 2 grams of meta toplin and need to know how to make a stock concentration and the best solvent to use can meta topolin be autoclaved? Can meta topolin concentration be stored over time?
You can learn about preparing stock solutions for plant hormones here: www.youtube.com/@PlantCellTechnology/search?query=plant%20hormone
Add meta topolin in the media after it's cooled down using the syringe filter technique.
great questions! I want to know now too :)
Im courious if they got contaminated or not
We will update you soon!
Tip. Do not use purple metho in a spirit lamp.
Please tell me the Hardening process
There are a bunch of vids on YT that teach acclimatization for TC. In my opinion, having a good humidity dome with vents is the best way to start.
This is what I use:
ruclips.net/video/L97ZFZ56pg4/видео.html
Here are some videos to learn about acclimation process: www.youtube.com/@PlantCellTechnology/search?query=acclimation
Hope you find them useful.
Is there a difference between 70% and 90% alcohol
70% alcohol is more diluted having 30% of water, whereas the 90% is more concentrated with 10% of water.
the higher % decreases the time it sits unevaporated which reduces the time its killing bacteria, it also helps getting in to the cells to have some water in it. be aware tho as alcohol isnt all that good at killing mold spores, unlike bleach solutions
The next step is for you to build a gene gun!
I'm going to try growing some Japanese red maple seeds in TC this winter. Is the protocol different for starting seeds? I read that tree TC usually uses 1/2 MS amount but no mention of seed protocol. 🫤
Yes, generally protocols differ based on plant species and even the explant used in the process. We haven't tried the species yet in our lab. But, would love to get some update from you if you are going for it. Good luck!
@@PlantCellTechnology Thank you! I just got three cuttings from my neighbor. I know that I should take them in the spring but he wasn't sure he still be living there next spring. Will give updates. 👍💚
Amazing!!
@@sweettooth5737
amazing!@@sweettooth5737