Speed Up Your Tissue Culture Media Preparation: Tips from Our Lab Director Francisco
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- Опубликовано: 8 мар 2023
- In this video, our lab director Francisco shares his top tips for making tissue culture media faster and more efficiently. Whether you're an experienced researcher or just starting out in the lab, these tips will help you save time and increase your productivity. Get the tissue culture products you need with 10% off using my code: www.plantcelltechnology.com/?...
Francisco covers topics such as selecting the right ingredients, optimizing sterilization procedures, and using specialized equipment to streamline your workflow. With his expert advice, you'll be able to produce high-quality tissue culture media in less time, so you can focus on your research and achieve your scientific goals faster. So join us for this informative and practical video, and learn how to make your tissue culture media preparation a breeze!
Don't forget to subscribe to our channel for hundreds of free educational plant tissue culture videos and be sure to mark your calendar for our upcoming Houseplants Tissue Culture Master Class with Francisco in Washington DC on March 25 & 26, 2023. This is a unique opportunity to learn from the experts and take your plant tissue culture skills to the next level. Join us and discover the amazing world of plant tissue culture today! plantcelltechnology.com/master-classes/
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Love this info 👏🏽👏🏽👏🏽💯 thank you
Thanks!!
Im totally new to this concept and very interested in learning more. Have you tried woody cultures? Any suggestions for growth regulators and rooting formulas?
We haven't yet worked with woody plants. But, if we do, we'll update you about it on our channel. so stay tuned.
Also, woody plants are tricky, so, be aware of the plant hormones you use and their concentrations.
When I would enter a lab as an undergrad, I'd always end up helping to make media, clean labware, and use the autoclave. I don't rightly know how many plates of media I've poured in total, but it was a lot.
Haha.. we all went through it, Victor. It's later on that we realize these processes are so crucial for experimentation.
Should i autoclave before all the equipment used in medium preparation ???
In media preparation you don't need to sterilize equipment, just keep them clean. Because after the media is prepared it will be autoclaved for the culturing processes.
Yes, whatever equipment you use during the tissue culture process need to be sterilized.
I have done tissue culture especially nodal culture but contamination is main problem. How can i get ride of this problem ?
Apart from following best practices to maintain a sterile environment, you can take the help of certain chemicals in your media that do not affect the cultures and do not react with media composition.
Plant preservative mixture (PPM) is one such product, have you tried that?
www.plantcelltechnology.com/plant-preservative-mixture-ppm/
Hey Francisco,do you perhaps have a video explaining how to prepare the different hormones from powder form into a liquid ?
Yes, we do have a video on preparing stock solutions for plant hormones. Please search on our channel.
@@PlantCellTechnology thanks mate :)
What is the alternative of PPM? I'm not finding it at my place
PPM is the only solution in the market like itself. That's why you are not able to find it anywhere. It's the only broad-spectrum formulation available in the market to fight contamination in tissue culture without affecting plants' growth.
What are the +'s and -'s of the Gellen vs Agar in your opinion? I work with cannabis if that matters to your answer. Thanks cheers
For your purposes, Agar is usually preferred, even for carnivorous plants. However, gellan gum is clearer (more transparent) when setting, so its easier to see the growth and spot for contamination faster, but this is recommended for houseplants.
Gellan gum vs agar depends on species. For some it does not make a difference and for some either can be better, meaning faster growth etc.
Thanks for this great video. You did not explain why you use agar gel in some deli containers and gum in the other
Just a test for me to find in which gel I get the better and faster response.
I wish you would use more sustainable containers instead of disposable deli containers. Thanks for the info, I have learn lots from you
I work alone with many plants, so deli containers make the process easier for me to work on a large scale. I don't need to wash vessels again n again before starting my process.
@@PlantCellTechnology I understand why you use it. And I get it, it’s a pain to clean all of it I deal with the same doing fungi cultures but it’s just more sustainable to reuse glass long term. Just my opinion and wish have a good one
@@adolfomoreno1771 Right Adolfo. Totally get it!
Pp5 screw caps containers may work well for you
Also make videos on m&s medium
We have multiple videos and blogs on MS media. What specifically are you looking for?
Hello, I have a question to ask: What is the temperature and time for sterilization of cooked food containers and bottles containing culture medium? In my case, the deli container will not withstand 121 degrees, it will deform, and the bottle containing the medium will be sterilized at 121 degrees for 40 minutes, it will become very yellow and will not solidify well. So I would like to ask you what your processing parameters are like.
You need to sterilize at 121 degrees for 20 minutes. This is the ideal condition. 40 minutes is way too much. Another reason for your culture dishes getting deformed would be the material they are made of. If the vessels are not made of autoclavable material they will deform. So, while purchasing from the seller ensure that the vessel can withstand high temperatures or not.
Polypropylene (PP) and polypropylene copolymer (PPCO) are autoclavable.
@@PlantCellTechnology Thank you for your answer. I vaguely remember that you mentioned in one of your works that you prefer to sterilize a bottle containing 1L culture medium for 40 minutes instead of the regular 20 minutes. Now I can't find that video, but if 20 minutes can also effectively sterilize 1L culture medium, that would be fantastic. I also need to try it out myself before I can use it with confidence. Thanks again!
Time varies due to differences in autoclaves. so, validation with your system is recommended. Though 20-30 minutes is what has been found to be enough by labs. @@user-yb7ry1ym9e
Can you please post your formula (ingredients) and measurements. You use a lot of acronyms and I am not sure I am catching correctly your measurements. Thank you.
For which plant do you need the media formulations?
I'm getting so much condensation in my containers that I can't see through it! It won't go away even after a few days. Any tips?
The main reason for condensation is temperature fluctuation.
You can try:
1. increase the distance between the light source and culture containers
2. wrapping with Parafilm or use an air-exchangeable jar
3. frequently clean the incubation surface with ethanol sprays
If it applies.... Let them cool to
Use bottom cooling if possible. Drop of 2 - 3 C is enough to completely eliminate the problem.
Hey Francisco , I wanna know that where should I sell these? I'm currently living in Pakistan. Kindly response me.
You can sell tissue cultrue plants to plant businesses, nurseries, or cultivators woking on small or larger-scale plant propagation. The best part is you can sell them in both forms, tissue cultured ones and acclimated ones.
Why not pour the media from bottles you wouldnt need to run as much but get more media density?
Hey Jason,
Can you please reframe your question? It'll help us to answer your question better. Thanks!
Keep it simple…
Do you have any questions or confusion at any stage? Let us know and we will help you with it.
whats ppm
Plant Preservative mixture. It's a robust formulation to eliminate contamination from your cultures.
@@PlantCellTechnology what is the chemical name of ppm.
Is Alpha naphthalenic acid and 6-benzylaminopurine solution an alternate for ppm
The names you have given are NAA and BAP, both of which are plant hormones and completely different from what PPM is.
Here's the info you might seeking for: chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/www.plantcelltechnology.com/content/PPMCOA2175.pdf
@@greenhut1642
@@PlantCellTechnology That you for your reply, I had searched a lot for ppm at our place. But it's not available in India. Is there any alternative for it. Or is it possible to send to india.
Yes! You can contact our local distributor. Here are their details:
Biogenuix
Main Office
Jyothi Shikhar Tower, Professor Joginder Singh Marg, Kirti Shikar, Janakpuri District Center, Janakpuri, New Delhi, Delhi 110058, India
91-9810029475
biogenuix.com
@@greenhut1642
I am in need and very curious on the growing medium used for nepetnthes./ carnivorous plants. How much of agar, sugar, ms basal medium, and kinetin. Please help me lol
we have some videos on Nepenthes TC on our channel that you may find useful: www.youtube.com/@PlantCellTechnology/search?query=nepenthes