thanks for the complement. Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
Thank you for this amazing video. One question: Has there to be screening between LR and BP reaction? I learned it a couple of years ago that after BP reaction the vector should be already used to transform E coli to amplify the vector and retain a high purity going into the LR reaction.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
In this video, you can see that the integration system involves the specific DNA sequences found on the phage, attP-sites, as well as sites found on the bacterial genomic target, attB. Recombination between the B and P elements results in new recombination sequences flanking the inserted phage DNA. After insertion, the recombination sequences are now called attL (left) and attR (right).
Please support our channel by using super thanks. Your 2$ donation would give us motivation to do more. Super thanks option is present below any of my videos, a heart sign with $ in it.
The BP clonase enzyme mix recombines attB sites with attP sites, generating attL and attR sites; whereas the LR clonase enzyme mix catalyzes the reverse reaction
By the way you can get this topic included in our animated biology website. The CSIR MCQ course has 10K+ mcqs with detailed solution (valid for lifetime) only @ 350 rs. Link: animatedbiologywitharpan.graphy.com/courses/CSIR-NET-Life-sciences-MCQ-practice-course-645881d4e4b0c11d957360ef
U r the best
Thanks a lot
Please share my channel link with your friends and help me to reach big audiance
ruclips.net/channel/UC4IpyopsGWSjaPACNTZLuqg please subscribe to my other channel as well 🙏
Done sir
Why cant directly clone gene to destination vector??
Thank you, sir. I was struggling with the concept. It's a great help for my exam on this Thursday.
Whenever you need to know something, some indian guy will explain that on youtube for you ❤ boy u my angel
thanks for the complement. Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Great explanation! Thanks a lot!
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Thank you so much for simple explanation
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Good explaination
Please share my channel link with your friends and help me to reach big audience
Thank you for your allways great explanations
Please share my channel link with your friends and help me to reach big audience.
Thanks for your kind words, for you guys I feel motivated....if you have any suggestions ,please put it in the comments
Excellently explained!!!
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
Excellent.
Please share my channel link with your friends and help me to reach out big audience
ruclips.net/channel/UC4IpyopsGWSjaPACNTZLuqg please subscribe to my other channel as well 🙏
your videos always help. thank you sir!
Please share my channel link with your friends...I am not getting much subscribers
Thank you for this amazing video. One question: Has there to be screening between LR and BP reaction? I learned it a couple of years ago that after BP reaction the vector should be already used to transform E coli to amplify the vector and retain a high purity going into the LR reaction.
Great video. Thank you!
Please share my channel link with your friends and help me to reach big audience
Thank you sir!
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Thank you! Hats off
Please share my channel link with your friends and help me to reach big audience
hey Arpan you make really good detailed videos, but your audio is peaking and may benefit viewers if you used a better mic. :)
Thanks for your suggestion. I will replace my mic . Please share my channel link with your friends and help me to reach big audiance
We are using the same donar vector in LR clonease so why attp1 attp2 is converted into attl1 and attl2?
Exactly what I wanted to ask. Maybe it adds additional nucleotides from the plasmid to become different site
Hey Arpan, great video! I am wondering how the gene of interest ends up being flanked by attL sites after it is incorporated into the donor plasmid?
In this video, you can see that the integration system involves the specific DNA sequences found on the phage, attP-sites, as well as sites found on the bacterial genomic target, attB. Recombination between the B and P elements results in new recombination sequences flanking the inserted phage DNA. After insertion, the recombination sequences are now called attL (left) and attR (right).
Please support our channel by using super thanks. Your 2$ donation would give us motivation to do more. Super thanks option is present below any of my videos, a heart sign with $ in it.
Thanks a lot.
Please share my channel link with your friends and help me to reach big audience
I wonder what the disadvantages are, since it is not always used.
bit expensive
Thank you 🌼
Please share my channel link with your friends and help me to reach big audience
@@animatedbiologywitharpan ok ı will share😊
I shared ın my wp class grup
Do the attb and attp sites remain intact? Or they also go recombination
The BP clonase enzyme mix recombines attB sites with attP sites, generating attL and attR sites; whereas the LR clonase enzyme mix catalyzes the reverse reaction
Please share my channel link with your friends and help me to reach big audiance
why BR to LR
Sir in which book i will get this topic
It's not in books . It a recent method
By the way you can get this topic included in our animated biology website. The CSIR MCQ course has 10K+ mcqs with detailed solution (valid for lifetime) only @ 350 rs. Link: animatedbiologywitharpan.graphy.com/courses/CSIR-NET-Life-sciences-MCQ-practice-course-645881d4e4b0c11d957360ef
Its getting updated every day. New MCQ notes and flash cards are uploaded all the time.