Hi there, love your videos and I have gone through all the Q&A and have not found a clear answer to the sugar question if you can please clarify. 1% VOLUME of sugar I believe is confusing for everyone. If we make a h2o2 solution at 1:19, let’s say 2cups/500ml, sugar isn’t really something anyone measures in “volume” so when you state 1% would it be safe to say add 1 tsp sugar (5ml)?? Please let us know, this process is something I will test on my old seeds! thrilled to have found your channel!
i used hydrogen peroxide to help germinate date palm seeds after i failed to germinate them three times. now they sprout like crazy! i was expecting like 40% success but i got about 80%. i was overwhelmed at how much date palm seeds that actually germinated and i love it 🥰
Thanks for the video! I have germinated seeds on peroxide before but I forgot the ratio, so started to search and found your video. I didn't know about adding sugar, that's an extra I will need because my seeds are old! Also you may check coppersulfate as an addition to your "seed reviving mix" as I remember having seen someone also using that in combination with peroxide to germinate very old seeds. Not sure if that is useful, but you may wanna check that out!
Two questions about some things I didn't understand well: First: how much would be 1% sugar, considering the solution is measured by volume and the sugar by weight? Let's say I have 500 ml of water, would that be like about a teaspoon? Or how can I calculate it? Second: won't soaking the seeds in the solution for several days waiting for them to sprout damage or kill the seeds? I've read in some other places that you're supposed to soak them for only a couple of hours, especially with smaller seeds.
1% solution means you have 1 gram of sugar in 100 milliliters (mL) of solution. As soon as you see the seeds sprouting in a couple days, you need to transfer them to fresh tissue culture media.
Hola, la solución de H2O2 con agua y azúcar, al momento de agregar las semillas, Se debería mantener en un contenedor oscuro? Yo usare semillas de tabaco por 24 horas. Saludos!!
1:42 Do I understand correctly that for 1 liter of water for germination you add 50 ml of hydrogen peroxide and 10 g of sugar? And how long, how many days can the seeds remain in this solution? A few days until they germinate?
Perhaps they recommend this concentration simply for sterilization, soaking for two minutes. Because if you leave the seeds for several days in a solution with such a high concentration of hydrogen peroxide, it can have a negative effect. Subsequently they will grow very slowly.
One question because I’m having trouble finding an answer online. Is the H2O2 method used ONLY for old seeds? Or can I use it on new seeds as an added way to get better germination results? I have used the baggy method with fairly good results, but I was wondering if I did a seed soak in a test tube and added the correct H2O2 to water ratio, would that help with a better germination rate. My issue is not knowing if H2O2 might in some way hurt or damage new seeds.
Yes, absolutely, you can use the technique for new seeds as well. Hydrogen peroxide is also an anti-fungal, so it will prevent the growth of mold in the seeds
I have 100's of heirloom species from 2012-2013... Is it possible that they'd grow up to be healthy and prolific?? I was about to throw them all away but paused.... many rare species of veggies and herbs...
I really wish I had seen this video before I tried sprouting some old cactus seeds I had. Anything would have been better than the zero germination rate I had.
I plan to plant bluebonnet seeds in the fall... they are know for slow germination due to seed coat hardness. Will soaking in this solution be of help...???
@@PlantCellTechnology ... Will do... I am preparing mixture using 1) 380ml of water, 2) 20ml of H2O2, and 3) 2gm sugar. Perfect fill for a 16oz plastic water bottle :)
@@PlantCellTechnology thanks for your response so just to be clear normal table sugar is added to the 19:1 ratio of hydrogen peroxide solution and this is the seed soak solution? How long shall i soak the seeds? I was checking some of your other videos on tissue culture & the gel you make to germinate the seeds in unfortunately I'm in Barbados some of those chemicals i'd have to ship in
@@geriannroth449 We haven't tried it yet but you can give it a go. After all, there're not too many ingredients involved. :) And, let us know the results if you do try on those seeds.
@@geriannroth449 You need to soak in until you observe germination in the seeds. And, then you will need to transfer to tissue culture media. You can purchase your tissue culture requirements from our online store. We ship worldwide. Visit www.plantcelltechnology.com
We didn't give it a try on eucalyptus seeds yet. But, maybe you can work with a few of them to see how it goes. It only requires H2O2, water, and sugar.
Sounds good, but not all are clear. "1 part of hydrogen peroxide with 19 parts of water", but what's the required concentration of hydrogen peroxide? In some sources I found info about 3% solution used to sterilize seeds and explants, and this is the concentration which is possible to find in local drugstores.
Hi there, love your videos and I have gone through all the Q&A and have not found a clear answer to the sugar question if you can please clarify. 1% VOLUME of sugar I believe is confusing for everyone. If we make a h2o2 solution at 1:19, let’s say 2cups/500ml, sugar isn’t really something anyone measures in “volume” so when you state 1% would it be safe to say add 1 tsp sugar (5ml)?? Please let us know, this process is something I will test on my old seeds! thrilled to have found your channel!
1% means 1 gram in 100 ml water.
thankyou very much! I have a scale I will adjust my solution and give it a go! Very Grateful for the reply!
@@offgridwithgrizz Absolutely! Happy to help!
i used hydrogen peroxide to help germinate date palm seeds after i failed to germinate them three times. now they sprout like crazy! i was expecting like 40% success but i got about 80%. i was overwhelmed at how much date palm seeds that actually germinated and i love it 🥰
That's so amazing. We're incredibly happy to learn about your success in germinating those seeds. Yay! 🥳🥳
@@PlantCellTechnologypls I only have 10percent hydrogen peroxide...how should I mix that...did not find 3
You can dilute it. Here are some answers on how to do it: www.quora.com/How-do-I-go-from-10-hydrogen-peroxide-to-3-hydrogen-peroxide@@saddammall3337
@@PlantCellTechnology I would like to germenite seed not for me ...
Thanks for sharing. New to your channel.
New to your channel too.🥳🌠🎇🎆👏
Thanks for the video! I have germinated seeds on peroxide before but I forgot the ratio, so started to search and found your video. I didn't know about adding sugar, that's an extra I will need because my seeds are old! Also you may check coppersulfate as an addition to your "seed reviving mix" as I remember having seen someone also using that in combination with peroxide to germinate very old seeds. Not sure if that is useful, but you may wanna check that out!
Absolutely! Thanks for sharing the idea. We'll give it a try!
How much copper sulfate do you think? 1 percent also?
Thank You
just wanted to confirm in this video you did a 1:19 ratio. in the video form a year ago you did 1:9. why the difference?
Right, I want to know too
Two questions about some things I didn't understand well:
First: how much would be 1% sugar, considering the solution is measured by volume and the sugar by weight? Let's say I have 500 ml of water, would that be like about a teaspoon? Or how can I calculate it?
Second: won't soaking the seeds in the solution for several days waiting for them to sprout damage or kill the seeds? I've read in some other places that you're supposed to soak them for only a couple of hours, especially with smaller seeds.
1% solution means you have 1 gram of sugar in 100 milliliters (mL) of solution.
As soon as you see the seeds sprouting in a couple days, you need to transfer them to fresh tissue culture media.
Fluval stratum…interesting. I never thought of using that but it makes total sense. That's what I have in one of my planted fish tanks.
This is great!
Thank you for your wonderful videos.
Thanks for your love and support.
Does this method also work for seeds that need cold stratification?
We haven't tried it yet with such seeds. You can definetly give it a go!
Gracias! ❤😊
Hola, la solución de H2O2 con agua y azúcar, al momento de agregar las semillas, Se debería mantener en un contenedor oscuro? Yo usare semillas de tabaco por 24 horas. Saludos!!
Have you tried experimenting with "corn sprout tea", as another method (or possibly in addition to H2O2 treatment) to help with old seeds?
Not yet.
thanks for sharing
Thank you for watching
Could you explain how you made the tc media?
It's an MS media supplemented with agar and sugar without hormones.
Is it better to use food grade hydrogen peroxide without the stabilizers? Or does it not matter that much? Thanks!
You can get 3% hydrogen peroxide from any grocery store. That will work.
Thank you so much!! I'm about to go add sugar to my seed water!!! 👍💚
Glad you found it helpful!
1:42 Do I understand correctly that for 1 liter of water for germination you add 50 ml of hydrogen peroxide and 10 g of sugar?
And how long, how many days can the seeds remain in this solution? A few days until they germinate?
Yes, I usually leave the seeds soaking for 12-48 hrs. I don't like to soak the seeks for longer than that.
I see many recommending a 6:1 ratio for germinating seeds. What do you think of that? Thanks!
That's good. But at least for sterilizing seeds to place in tissue culture, I found that 19:1 works for almost all the seeds. I have tested.
Perhaps they recommend this concentration simply for sterilization, soaking for two minutes. Because if you leave the seeds for several days in a solution with such a high concentration of hydrogen peroxide, it can have a negative effect. Subsequently they will grow very slowly.
One question because I’m having trouble finding an answer online. Is the H2O2 method used ONLY for old seeds? Or can I use it on new seeds as an added way to get better germination results? I have used the baggy method with fairly good results, but I was wondering if I did a seed soak in a test tube and added the correct H2O2 to water ratio, would that help with a better germination rate. My issue is not knowing if H2O2 might in some way hurt or damage new seeds.
Yes, absolutely, you can use the technique for new seeds as well. Hydrogen peroxide is also an anti-fungal, so it will prevent the growth of mold in the seeds
Once the seeds germinate in the peroxide solution, can the seed be planted directly into soil or seed starting mix? Or does it have to go into TC?
This info is already mentioned at the end of the video. You can either place the seeds in TC or soil.
What's that translucent gel in the bottom of the cups?
Media with agar.
I have 100's of heirloom species from 2012-2013... Is it possible that they'd grow up to be healthy and prolific?? I was about to throw them all away but paused.... many rare species of veggies and herbs...
Yes, of course. You can try this method.
I really wish I had seen this video before I tried sprouting some old cactus seeds I had. Anything would have been better than the zero germination rate I had.
Hello friends, I needed blue berry seeds germination,
I plan to plant bluebonnet seeds in the fall... they are know for slow germination due to seed coat hardness. Will soaking in this solution be of help...???
We haven't tested it yet on the seed, but you can give it a try as it only requires hydrogen peroxide, water, and sugar.
@@PlantCellTechnology ... Will do... I am preparing mixture using 1) 380ml of water, 2) 20ml of H2O2, and 3) 2gm sugar. Perfect fill for a 16oz plastic water bottle :)
@@adctex That's great!! Ensure the correct ratio.
Can we use stabilized hydrogen peroxide 3% from pharmacy for seed germination? Can stabilizers inhibit germination or damage seedlings?
yes, absolutely!
will this work inplace of cold stratification
You want to combine this technique with cold stratification?
duration time of soaking seed in hyrdogen peroxide solution?
The seeds will sprout in a couple days. So, the answer will be until they sprout.
Is it parts by volume or weight? 01:43
It's parts by volume.
do these have to be kept out of the sunlight while they germinate?
Germination doesn't need sunlight
What kind of sugar do you use white sugar, brown sugar or glucose. Have you soaked the seeds in a solution of hydrogen peroxide & sugar?
You need sucrose for the process. so, table sugar will also work. yes right, the seeds were soaked in the prepared solution.
@@PlantCellTechnology thanks for your response so just to be clear normal table sugar is added to the 19:1 ratio of hydrogen peroxide solution and this is the seed soak solution? How long shall i soak the seeds? I was checking some of your other videos on tissue culture & the gel you make to germinate the seeds in unfortunately I'm in Barbados some of those chemicals i'd have to ship in
@@PlantCellTechnology would this solution work for seeds with really hard dense seed coats like guava & some palm seeds?
@@geriannroth449 We haven't tried it yet but you can give it a go. After all, there're not too many ingredients involved. :) And, let us know the results if you do try on those seeds.
@@geriannroth449 You need to soak in until you observe germination in the seeds. And, then you will need to transfer to tissue culture media. You can purchase your tissue culture requirements from our online store. We ship worldwide. Visit www.plantcelltechnology.com
How much time seeds should be soaked in hydrogen peroxide?
Until you see it sprouting, which you observe in a couple of days.
@@PlantCellTechnology thank you so much for the quick response
@@shitaldesai4691 Happy to help!
Will it work on the finest seeds like baby dollar eucalyptus.
We didn't give it a try on eucalyptus seeds yet. But, maybe you can work with a few of them to see how it goes. It only requires H2O2, water, and sugar.
Sounds good, but not all are clear. "1 part of hydrogen peroxide with 19 parts of water", but what's the required concentration of hydrogen peroxide? In some sources I found info about 3% solution used to sterilize seeds and explants, and this is the concentration which is possible to find in local drugstores.
As mentioned, Francisco uses 3% commonly found in local drugstores or grocery stores. Hope this helps you!
@@PlantCellTechnology thanks for the clarification! Maybe I just missed it watching this video
3 percnt is common but some have 12 percnt aĺl guids are side off bottle
IS 1% HYDROGEN PEROXIDE OK FOR 5 YEAR OLD CANIBUS SEEDS? AND IF UR MAKUNG A 100ML MIX HOW MUCH SUGAR DO U USE & WHAT TYPE OF SUGAR.
THANKYOU
LOUIS
Yes, it will help, but we can't guarantee it will work. Also, we use 1% sugar.
@@PlantCellTechnology Thankyou
@@PlantCellTechnology thankyou
@@louisel-khouri1015 Of course!
Happy to help!
How to germinate cucumber 4 year's long seeds???? Please reply
Hi,
We haven't tried the technique with cucumber seeds yet.
@@PlantCellTechnology please give any suggestions sir,,,,
I tried so many techniques but not germinated
@@Kavya333If we find any relevant info regarding growing cucumber seeds, we will share them with you.
@@PlantCellTechnology Thank you so much sir....
do you use any Hydrogen Peroxide or food grade Hydrogen Peroxide?
No need for food-grade peroxide.
Will sugar cane work?
We haven't tried it yet. but you can give it a go as it only requires three components.
@@PlantCellTechnology what kind of sugar should I use if you don’t mind me asking
You can either use sucrose used in labs or table sugar. @@DillMan-z8x
@@PlantCellTechnology thank you!
❤😊
If the peroxide doesn't work then bust out the GA3.
do these have to be kept out of the sunlight while they germinate?
No, after germination I transfer them to tissue culture media.
How long do you keep it in the water?
We don't keep it in water. After the seed starts germinating, we transfer it to tissue culture media. @@OfficialReysace