Ma'am adaptor k case me blunt end kaise. Gene of interest pehle se hi blunt end hai na ligation k liye sticky ends chahiye as vector b sticky ended hai. Please clear doubt
Ma'am the lecture was quite good.. but I want to know that what is the size of linkers and adapters..as you have told it is oligonucleotide then I think it is around 10-12 bp... Is it correct??
Are you sure? Ya phir overhangs me hi bam H1 ka sticky ends mention hai means two adaptors ligate hoke restriction site form hoti hai thn we can digest it using that particular restri. Enzymes? So will get the sticky ends. I don't knw if this is correct.
During adapter addition to vector THE vector should have sticky end but in this video the vector shown to have blunt end, I hope mam you will read my comment and notice this mistake.
Hello mam.. You are the best.. I can easily understand.. But.. In this, Adaptors convert blunt ends to blunt ends.... And in your explanation, Adaptors convert sticky ends to blunt ends.... ?????? Sorry mam.... Bt i can't get it.. Please say something about this.. Please mam..
I used to watch your videos previously but this video is now creating trust issues because u didn't completed last line clearly adaptors can join and ligate themselves by complementary base pairing but u didn't told how to prevent it and I am going to write same in my exams also but thank God i saved as I cross checked on google and fount that there is also procees ahead to prevent ligation of adaptor molecules it is wrong that u didn't sorted that topic of adaptors clearly..... Firstly try to check your own content before Posting something 🙏
Ma'am the clarity with which you explain the concepts is awesome..... thanks a lot for helping so many students out there😊
Thank you Sapna
Again thanx madam...you are uploading those videos which we need in upcoming days..lots of love from Kashmir 💕💕💕💕
Mam you are best teacher 🙂
Mam m your grt fan mam I love your lecture tq so much🤗🤗🤗🤗🤗🤗
Informative lecture ❤
Wonderful maam , the way of explanation
Glad you liked it
Amazing...amazing..amazing
You explained very well thank you!
Thank you ma'am 🙏🫂❤️
Wonderful mam❤
Thanku so much mam💫💫💫🧿🧿🧿🥰🥰
I just love your explanation..keep it up.. I request you for videos of bacteriophage vectors
Thank you. From Bangladesh.
Thank u ma'am❤
Thank you ma'am for such crystal clear explanation
Love you & like you.from Bangladesh
Ma'am adaptor k case me blunt end kaise.
Gene of interest pehle se hi blunt end hai na ligation k liye sticky ends chahiye as vector b sticky ended hai.
Please clear doubt
Adaptors also convert blunt end to sticky end. Thank you.
the clarity with which you explain the concepts is awesome..... thanks a lot for helping so many students out there😊
Great video!
Thank you ma'am
Mam ur videos are more helpful for us plz upload more videos from TA brown..
Thank you ma'am for teaching us 😊
Mind blowing.. 😊
Thanks a lot...its main topic
Bahut achhe mam
Ma'am adaptors are used to covert blunt ended fragments into sticky ends
Absolutely right
Madam opposite bta rhi hi
ma'am please make a video on proteins for transcription in prokaryote vs eukaryote just like u made one on replication
mam u told that adaptors work in vectors for sticky ends but will it work on GOI with same ends?
Please make a video that how we can construct a plasmid using any software
Tnx a lot mam
very well explained..
Very nice lecture
happp teachers day,,,,,,,,,,,,,,,mam
Hello mam very nice explain
Very nice
Thank you so much mam
Mam please video on transcription from watson book🙏🙏🙏
What is the other ways to joining DNA molecules accept dna ligase..??
Ma'am the lecture was quite good.. but I want to know that what is the size of linkers and adapters..as you have told it is oligonucleotide then I think it is around 10-12 bp... Is it correct??
Jazakallah
Nlyc explanation
Simple😄
Mam animal biotechnology ke upar video banaaiye please
Hai mam...can you explain about the amino acids please madam
Thanks mam
the function of adaptors is incorrect they are used to produce sticky ends not blunt ends
Are you sure?
Ya phir overhangs me hi bam H1 ka sticky ends mention hai means two adaptors ligate hoke restriction site form hoti hai thn we can digest it using that particular restri. Enzymes?
So will get the sticky ends.
I don't knw if this is correct.
please make more videos plz plz
What about homopolymer trailing mam?
Mam DNA cloning per short skich upload kary plz ki akhir my ham kia kari
Plz
Ma'am why can't we clone unknown gene sequence by using linker
Mam gene cloning and dna cloning me kya different h
mam pls recommend best book for biotech🙏
for RDT it is TA brown
@@BaaYo thank u mam
Didi yeah adaptor mean blunt ko blunt kardia yeah SMj ni aaya
Adapters ki requirement hi kya h
Hello ma'am,
My brother has completed his Bsc in Biotechnology. Can he do Msc in Chemistry after taking Biotechnology. Pls reply maam .
if chemistry was a subject in BSC then i think he can apply..
💙
Thank you very much Ma'am
During adapter addition to vector THE vector should have sticky end but in this video the vector shown to have blunt end, I hope mam you will read my comment and notice this mistake.
Didi aapne konsi padai ki hai
Hello mam..
You are the best..
I can easily understand..
But..
In this, Adaptors convert blunt ends to blunt ends....
And in your explanation, Adaptors convert sticky ends to blunt ends....
??????
Sorry mam....
Bt i can't get it..
Please say something about this..
Please mam..
Mam can u please upload in english mam...bcoz every student can be able to understand easily english mam......this is my small request mam
Please mam riplay dijiye
1day 2video uploaded
I used to watch your videos previously but this video is now creating trust issues because u didn't completed last line clearly adaptors can join and ligate themselves by complementary base pairing but u didn't told how to prevent it and I am going to write same in my exams also but thank God i saved as I cross checked on google and fount that there is also procees ahead to prevent ligation of adaptor molecules it is wrong that u didn't sorted that topic of adaptors clearly.....
Firstly try to check your own content before Posting something 🙏
Pls upload elaborately chromosome walking..mam
Your explanation about Adaptors molecules was wrong. 👎
taught wrong thing in adaptor.Adaptors have pre performed sticky ends.
🤨😒
Thanks mam
Mam industrial biotechnology ke upar video banaaiye please