Instructable DIY antibiotics #1: making agar plates

Our (future) medicines are everywhere. In a series of three video's, Waag Society explores new resources surrounding antimicrobial resistance. Biohacker Pieter van Boheemen invites you to Waag Society’s Open Wetlab to learn how to hunt, grow, and test new medicines. Why? Antimicrobial resistance is much more a social than a technical challenge. It is a direct result of our increasingly urbanized society, fed by large scale animal farming, equiped with centralized waste water treatment, and abuse of prescription drugs.

[@gabrielgan2971]

Thanks for the video, very easy to understand, hope you can make more of these kinds of videos

[@RobCLynch]

We should learn this for when Coronavirus brings down civilisation.

[@tomfennings8384]

Robert C Lynch holly shit bro , yes that is why I’m here preparing lol 😝

[@AwesomeAsh99]

@@tomfennings8384 y'all been reading too much World War Z

[@gogodaal7273]

Pam Bennett the fuck you people still believe this shit

[@ameliecarpenter2287]

Umm, covid 19 is a virus so antibiotics would not help at all

[@Rxzwell]

@@ameliecarpenter2287 95 percent of the population has no idea the difference between viruses and bacteria, antibiotics have been prescribed for viruses for decades now because alot of people wont leave the doctor without a pill and big pharma can care less because they make a buck, ignorance will be our downfall, nothing we can do but hope these people pick up a book and read rather than watch, tik tok, fear mongering news owned by the gov, or watching YT channels with hidden agendas

[@mattberry8046]

I love this guy it feels like I'm being taught practical bio by a young "Ewen Bremner, Spud from Trainspotting".

[@yuiopoli9601]

Thank you!!!!!!!

[@jasonloges6030]

What nutrients are they

[@waffleMccoy]

I love this guy

[@ithinkimarealboy2402]

Dear OP,
Good video but you need to add more details... How hot is the temperature on the pressure cooker? A few other things but mainly just be a little more specific with your data and measurements otherwise great educational video!

[@daniellemorrison7154]

👀

[@jamesbondaygee]

How effective are these anti-biotics and treating various sicknesses. Occasionally I get bronchitis...doesn't seem to go away unless I get an antibiotic so this an important question as our civilization is crumbling.

[@CUBETechie]

2:42 so how much you would need ? Roughtly the same amount of both?

[@98karlh]

Remember to add water to pressure cooker :)

[@adityasuresh6607]

Nice
You won't believe how many people forget that
Seriously.

[@irfana19nov]

😀

[@jason8ification]

What was the nutrients required?

[@echodelta.foxtrot7718]

Is this a usable antibiotic?

[@galacticchad2069]

Me after watching DR STONE:

[@elisafinch1325]

what is agar,you put the bottle in pot..with water?.what cain of nutrient?..where we can will buy that?....thank you..lovely person you are..to share..Elisa Finch

[@uberawsome3696]

Agar with nutrients is kind of like a food jello for cell cultures.

[@carlaceron9983]

So....do you just like eat it and we good? Don't you need to have a proper dosage?

[@magiclee9482]

Dont you get contaminations due to air exposure?

[@pietervanboheemen]

Nope. Because of the torch all the air arpund the plates got sterilized.

[@StephenBlacktampa]

Great info

[@christianmcbain810]

This is very poor sterile procedure! No gloves, no hand washing, and that torch is doing absolutely nothing to prevent contaminates in the air from landing on the dishes while he pours the media. He also doesn’t explain that you must pour the plates while the gar solution is still hot so it doesn’t congeal in the bottle. Normally agar work is done under a flow hood, still air box, etc. doing your work like this can work but you’ll have A LOT of contamination issues,

[@jaredstone9487]

@@christianmcbain810 you suck

[@ISawYouMushroomHead]

@@jaredstone9487 Christian is right

[@themaximusone]

nutrients ??? what kind that is a BROAD SPECTRUM my friend

[@pambennett8967]

Why doesn’t it melt the lid?

[@AaronAlso]

At full pressure most PC's only go to 15psi(103Kpa); that is the same as 250degF. Many plastics such as polypropylene (lid) and polystyrene (petri dishes) have melting points above this tempt and are safe for use in a pressure cooker or autoclave. Additionally, agar is generally a "clean" substrate and doesn't require deep sterilization; usually a lite pasteurizing is sufficient - steam bath in a pasta cooker.

[@RetiredRhetoricalWarhorse]

You removed the petri dishes with bare hands... I'd assume they're contaminated whether you close the bag or not.

[@christianmcbain810]

Exactly!! Plus that torch is just causing a sterile cone of air above the flame! It does nothing to sterilize all the air in the room and unless that room is sealed really well with HEPA filtered air system there’s tons of contaminates floating around. He didn’t even wash his hands!

[@funny11744]

Question : why antibiotic îs put in Agar ?

[@AaronAlso]

When you want to grow things other than bacteria.

[@funny11744]

@@AaronAlso many thanks

[@guardianofthesacredflame1649]

That was suppose to be fire in the hole

[@ivanguthrie0420]

no flow hood? yup move slow.

[@daniellemorrison7154]

👀

[@iamsebv]

La wea mala

[@SC-qg8wp]

neeeeeeeeeerd

[@okperson9771]

ultra nerd

[@okperson9771]

you had to reply in 2 months? I bet you are one sad person.

[@michaelolafitness9491]

The Debunker danm

[@okperson9771]

nerd nerd nerd

[@okperson9771]

nerd