Instructable DIY antibiotics #3: observing the zone of inhibition
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- Опубликовано: 7 сен 2024
- Our (future) medicines are everywhere. In a series of three video's, Waag Society explores new resources surrounding antimicrobial resistance. Biohacker Pieter van Boheemen invites you to Waag Society’s Open Wetlab to learn how to hunt, grow, and test new medicines. Why? Antimicrobial resistance is much more a social than a technical challenge. It is a direct result of our increasingly urbanized society, fed by large scale animal farming, equiped with centralized waste water treatment, and abuse of prescription drugs
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As a PharmD I found this fascinating! Thank you for sharing!
Awesome! Have you been compiling a list of the plants that have worked?
This is really interesting. It would have been cool to recreate the first antibiotic from oranges
Whoa, amazing! I hope the microbiocidal or microbiostatic effect is not due to Ethanol.
He did boil the solution in the other video so if there is any ethanol left in the extract at all it would be greatly reduced.
@@Blimbus-Blombo Hahahah! That is what I thought. Some people said that piece of paper is too small, alcohol evaporates. What they do not consider is in that too small of a chamber is very moist, relative humidity is 100%. Evaporation is extremely slow. Compound with that, there is a mountain of evidence of alcohol evaporates much slower if mixed with water. The alcohol is used is 96% alcohol, I presume it is for laboratory use so the rest of 4% must be water.
All his demonstrations are not true-to-life. It is all about the idea rather than the real thing. By 'real thing' I mean his demonstrations assumes everybody already knew what he is demonstrating. Not much explanation the how and why. Which defeats the idea because people watching his demonstrations watch his demonstrations for a reason -- they do not know.
There are people who take things literally and wonder why they fail! For example, watch RUclips making chocolate cookies and follow exactly without tweaking nor considering different variables. I live one person like this! Saying that they are stupid is an understatement. Zero brain cell. Zero common sense. You tell them to jump, they ask you how high.
Great video! I did this stuff in HS and loved it. Time I think to try it again.
turning on a butane torch wont sterile the air in a room or the air near it. in fact the heat will case air currents in your immediate workspace. as the air heats up and rises the sounding cold air comes in to take its place.
Pieter, please do a video on biohacking insulin for diabetics. Thanks!
this would be a great idea!
it will disrupt the billion dollar insulin industry and he'll definitely would not like to get killed in a mysterious way 😂
isn't it because of the alcohol in the extract? Please verify it with other extraction methods. Or maybe do it with an alcohol solution side by side and observe if it gives the same result.. that would be a cool video. I'm curious if there is a more conclusive standard procedure in the pharma industry..
I thought the same thing, I’d probable use water, also there is bleach in white printer paper
Questions:1. Temp of the incubation chamber. I saw 24C/75.2F, but the human body is 37C/98.6F. Why not use this temp as this is the temp of the human body?2. After extraction with 96% alcohol...can you add distilled water and reheat to further evaporate all the alcohol out because alcohol is a antiseptic? This might increase the chance of a positive result?3. How long can you store the extracted antibiotic? Would you freeze it?4. How do you know what illness to use it for? 5. How do you administer and figure out the dosages?Thank you and awesome video.
I'm no expert but you never let bacteria incubate at body temperature because it may breed a bacteria that is exceptionally harmfull to humans that lives comfortably in our body temperature.
I’ll comment on what I know with reasonable certainty
1. The above answered that question, also I want to add that a lot of pathogens are a lot more resilient. Decreasing temperature generally just slows down their growth. You have to be careful to not increase it too much/too fast.
2. In theory that would be totally fine
3. Freezing would potentially preserve the most yield, most sensitive compounds are stored at -20 or -80 C in my experience
4. The only way to know for sure is to isolate specific pathogens and test the efficacy of the antibiotic per pathogen. You can driving a plate into quarters and spread different bacteria into each quarter and see if you observe the zone of inhibition against that species. You can also get a good estimate if you know the class and pharmacology of the molecule(s) you are working with. But that requires knowledge of your extract that’s not easily discovered
5. My best guess is a trial on mice to understand what dosage is effective in stopping infection in x% of the population
Dosage is generally measured as milligrams of drug per kilogram of body weight so you can scale up from there (although you want to do trials to make sure it’s safe for humans before use/consumption)
These compounds kill living organisms, your cells might also be susceptible depending on what the antibiotic is. Penicillin poses no risk to your body u less you are allergic, but sulfa drugs (TMP/SMX) can cause severe anemia if not used properly!
How can I join you? I have an MS in Chemistry and I did research on the plant extracts, characterize the organic compounds found in that plant and performed antibacterial activity. Now I wanna proceed ahead with this research to find new antibiotics.
Please guide me.
Regards from Pakistan
Question, the small paper circle that you put into the petri dish contains flower extract and alcohol. How do you know if the bacteria is not just avoiding the alcohol?
The alcohol is already gone after he boiled the extract in part 2
Please post on some more methods of extraction, and purification of compounds.
so how does someone consume or use this for personal benefit?
Don’t touch them with your hands? 🤔 didn’t you fold the paper with your hands to punch holes? Still love the vids. Thanks for sharing
I kind of would like to see if I could do this and test the colloidal silver that people say can kill viruses and bacteria
Hi, your video was awesome, add some microscope videos as the antibiotic eliminates the microbes, if you are going to make more.
Where did you get the microbes?
Doorknob probably
wouldn't any alcohol extract be anti-microbial because, you know.... alcohol...
Yes, alcohol is an antiseptic... that's why it's important to make sure you've driven out all of the solvent. Might produce a false-positive test result.
I think he said he used 96% ethanol. After the ethanol evaporates, the extracted compounds are dissolved in the remaining water.
How would i extract a flavour and inserted it in to another plants
How would I know the proper dosage? Would one of those small vilest be considered 1 dose for an adult?
aaawwweeesssooommmeee
Try penicillin next
So is the plant extract actually usable?
it's not that simple as he say. It could kill bacteria and also kill humans as well 😂 test it with animals. Oh heck how can we progress the science with animal rights people. 😂
What kind of microbe did you use?
It’s in the previous video. It’s from an orchid planet.
@@YoYoRaeRae How do I get the microbes from an orchid planet if I don't have a spaceship?
hi, john.
there are many notices.
where is Glooooovs? ,
piking up filter paper disc from the bench without autoclaving it,
microbial inoculum concentration should be according to MacFarland standard,
direct subculturing is wrong,
He "sterilized the air" by having a flame somewhere, so it's fine.
I would love to help in the search but unfortunately live very far away. Is there anything I can do to help?