Crispr Cas9 & Fluorescent Yeast: Genetic Engineering at Home

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  • Опубликовано: 2 ноя 2024

Комментарии • 149

  • @AuditionSerenbe
    @AuditionSerenbe 4 года назад +7

    OMG thank you SO much for making this! I ordered the ODIN kit for my kids and I to do and we could not for the life of us figure out how to do it based on the instruction booklet. THIS was what we needed! I know that took a ton of time to create but this family is SO grateful you took the time to do it! Much appreciation!

    • @Jimiticus
      @Jimiticus  4 года назад +2

      My pleasure! I hope it works out for you!

  • @melvin0o708
    @melvin0o708 6 лет назад +33

    Probably one of the most underrated education channels out there. Keep up the awesome quality videos!

  • @qqqalo
    @qqqalo 4 года назад +6

    This channel had so much potential. Hopefully you may find some extra time during quarantine and make a new video. Keep it up man.

  • @debodatta7398
    @debodatta7398 Год назад +1

    This guy casually comes onto youtube drops 2 really interesting vids on the Canadian accent followed by 5 legit GOATED videos back to back including one of the most well done animations of a rome video ever put on youtube and dips... incredible one of the most talented creators for sure super underrated

    • @Jimiticus
      @Jimiticus  Год назад

      oh wow, thanks for the compliment!

    • @vinniepeterss
      @vinniepeterss 7 месяцев назад

      ​@@JimiticusYou're still alive?

    • @vinniepeterss
      @vinniepeterss 7 месяцев назад

      ​@@JimiticusWhy r u stop producing videos mate?

  • @borkbork6059
    @borkbork6059 5 лет назад +4

    No matter what happens to this channel, I know I learned so much. Thank you!

  • @Nikotin-lu1xo
    @Nikotin-lu1xo 5 лет назад +33

    So, is this channel dead? Pretty sad, cause the videos are really well-made

    • @Jimiticus
      @Jimiticus  5 лет назад +8

      Thank you!

    • @zapthebird8141
      @zapthebird8141 5 лет назад

      Well I might be doing more shoutout videos for my uncle here because his channel shall never die 😜

    • @Jimiticus
      @Jimiticus  5 лет назад +2

      😀😀👍

    • @jameilious
      @jameilious 4 года назад

      @@Jimiticus Please more! I love science videos and yours still manage to be on topics I've not seen anyone else do videos on. They're also very well made!

    • @Jimiticus
      @Jimiticus  4 года назад +6

      Thank you! I do want to make more eventually

  • @Pwn3dbyth3n00b
    @Pwn3dbyth3n00b 4 года назад +1

    Man I miss this channel. Come back please

  • @songbirdrohan3973
    @songbirdrohan3973 5 лет назад +4

    Dang Bro, this channel was growing so fast, i wish u kept going, your channel would have been popular overtime.

  • @mna159
    @mna159 3 года назад +1

    Please continue doing such Top Rated Tutorials. Make it easy. You rocked it 👌

  • @simonthor7593
    @simonthor7593 6 лет назад +13

    Great video as always!

  • @fernandoagz1973marzo
    @fernandoagz1973marzo 6 лет назад +5

    Nice video!
    Just a a couple of things... Perhaps, you have a lot of bacterial colonies in your plate of LB/Strep because Strep degraded after microwaving (like Kanamycin in your YPD plates). So, you can´t tell that the CrisprCas worked.
    Next time use a control plate for bacterial cells without CrisprCas plasmids... if you see colonies in these plates there is something wrong with the Streptomycin plates.

  • @therealjoedart
    @therealjoedart 6 лет назад +1

    Excellent job. Hearing the details was very informative. And the low glow final result was a lesson too.

    • @Jimiticus
      @Jimiticus  6 лет назад

      Thanks! Sometimes it's fun when things don't go perfectly, then you get to try to figure out why!

  • @Some-wr7mi
    @Some-wr7mi 6 лет назад +13

    Forgot about this channel

    • @Jimiticus
      @Jimiticus  6 лет назад +1

      I'm still here...for now at least :)

    • @ErikB605
      @ErikB605 6 лет назад

      You still there?

    • @Jimiticus
      @Jimiticus  6 лет назад

      Who me?

    • @ErikB605
      @ErikB605 6 лет назад

      Yep. Weeded out my subscriptions. You seem to still be alive. Good to hear :D

    • @Jimiticus
      @Jimiticus  6 лет назад

      Next couple videos may be hosted elsewhere, but I'll make a short video saying where

  • @SimplifyIt02
    @SimplifyIt02 6 лет назад +3

    Love watching your videos

    • @Jimiticus
      @Jimiticus  6 лет назад

      Thank you for watching!

  • @ismaelferreira1055
    @ismaelferreira1055 3 года назад +1

    Awesome quality

  • @DiegoChurch
    @DiegoChurch 6 лет назад +3

    i already bought the gene engineering kit, gonna be running experiments soon

    • @Jimiticus
      @Jimiticus  6 лет назад +1

      Good luck!

    • @MA-eo9rl
      @MA-eo9rl 6 лет назад

      How did it go ? I consider buying one too

    • @adeelmehmood7439
      @adeelmehmood7439 2 года назад

      @@Jimiticus I have genetic polycystic kidney disease (pkd) by mutations. These pkd genes called faulty genes. So my question is for you can it cut faulty gene by Crispr-cas9 protocol. May Crispr-cas9 is there only one gene protocol and use for all faulty genetics diseases thanks

    • @Jimiticus
      @Jimiticus  2 года назад +1

      @@adeelmehmood7439 I am so sorry to here that. I'm just a filmmaker who is interested in the science, so I'm not qualified to answer your question.

  • @listenhere2006
    @listenhere2006 Месяц назад

    yo youre the best of the best so much so clear mad respect

  • @ali._.hallani
    @ali._.hallani 4 года назад +1

    I would love to see more videos, great stuff

  • @TonyKlusacek
    @TonyKlusacek 6 лет назад +3

    awesome! i´ll try to get one kit! Thks!! very good explanied info

  • @Insipedia
    @Insipedia Год назад +1

    Hey, out of curiosity I was wondering, how long did it take for your lightspeed video to go viral? Like did it get 1 million views within a month? I'm trying to use your stats and compare them to my new video (similar to your channel's videos).

  • @farhanahmed2508
    @farhanahmed2508 6 лет назад +2

    Really cool. I wish that this was available in countries other than the US too...

    • @Jimiticus
      @Jimiticus  6 лет назад +2

      The kit? It might be, you can send them an email to double check. I'm in Canada and was able to get the kits. It took awhile but there wasn't any problems with the shipping.

    • @allie705
      @allie705 6 лет назад

      They do deliver outside the U.S!!

  • @AdityaSharma-ox5jy
    @AdityaSharma-ox5jy 3 года назад +1

    You have a video with 7 MILLION VIEWS!!! Why didn't you make more videos lol?!

  • @ivo2345
    @ivo2345 5 лет назад +2

    You could've just picked out the glowing patch and placing it on a new agar plate to let only the glowing patch to duplicate so it could've been a success with a little more patience. Still good work dude.

    • @Jimiticus
      @Jimiticus  5 лет назад +1

      Yeah I wish I did that afterwards, but I wanted to have a successful experimental from beginning to end, my goal wasn't just the glowing yeast for its own sake

    • @alexanderher7692
      @alexanderher7692 5 лет назад

      can this be done to produce CBD?

  • @saulsh5900
    @saulsh5900 4 года назад

    Beautiful explanation minds like this is what we need thanks.

  • @andrewmclelland3657
    @andrewmclelland3657 3 года назад +2

    Did this video change your life ? I’m curious as you produce some tremendous content I can’t understand why you have stopped and hope for you to go again 🙏

    • @Jimiticus
      @Jimiticus  3 года назад +1

      Not enough views on my videos for the time commitment, especially with a full time job. Maybe one day... :)

    • @AdityaSharma-ox5jy
      @AdityaSharma-ox5jy 3 года назад +3

      @@Jimiticus You did get a video with 7 MILLION VIEWS!!! If you make more videos in general, your watch hrs will increase and all your videos will sky rocket in views. You have a lot of views for the amount of videos you have on your channel. I've never seen that kind of accomplishment.

  • @s.i.rosanakthar1857
    @s.i.rosanakthar1857 4 года назад +1

    put more stuff related to this

  • @SirLaurenceNZ_2
    @SirLaurenceNZ_2 6 лет назад +1

    Nice tip!

  • @DavidL-wd5pu
    @DavidL-wd5pu Год назад

    Love diy science!!

  • @DarlinMarleneVeVo
    @DarlinMarleneVeVo 4 года назад

    thank you!

  • @AdityaSharma-ox5jy
    @AdityaSharma-ox5jy 3 года назад +1

    How did you learn to make such good animation?!!!

    • @Jimiticus
      @Jimiticus  3 года назад +1

      Decades of practice 😀

    • @Jimiticus
      @Jimiticus  3 года назад

      nearly 40 :)

    • @AdityaSharma-ox5jy
      @AdityaSharma-ox5jy 3 года назад

      @@Jimiticus Wow. Were you a former animator or something?!

    • @Jimiticus
      @Jimiticus  3 года назад

      No, I just like to learn various filmmaking techniques.

  • @mohannd1234
    @mohannd1234 5 лет назад +1

    Amazing, subscribed gj

  • @GeorgeTrialonis
    @GeorgeTrialonis 6 лет назад +2

    Very informative video, thank you. Could I ask what software you are using for introducing the graphics and animations in your video? Thanks.

    • @Jimiticus
      @Jimiticus  6 лет назад +1

      Thanks. I used the Adobe Creative Cloud suite, so photoshop, after effects and I edited this one on Davinci Resolve, more as a test, I normally edit on Premiere. I also created some of the graphics drawing on the iPad with the adobe apps.

  • @AdityaSharma-ox5jy
    @AdityaSharma-ox5jy Год назад

    What did you use to record the audio? Phone? Mic?

  • @clownavenger0
    @clownavenger0 5 лет назад

    very interesting. Is it done in a similar way when used for practical results?

  • @mukynas
    @mukynas 5 лет назад +1

    Genetic Engineering:
    10% fun stuff
    90% discard the tip

  • @geraldallistair4031
    @geraldallistair4031 4 года назад +1

    why did he stopped posting?

  • @thomasevans5467
    @thomasevans5467 Год назад

    Did you use distilled water?

  • @aaravahluwalia2877
    @aaravahluwalia2877 6 лет назад +4

    Nyc information 👍😉

  • @Slipee-me8bi
    @Slipee-me8bi Год назад +1

    Glow in the dark bread 🍞

  • @gregsmith6756
    @gregsmith6756 4 месяца назад

    Why not just re-culture the one colony that did take up the plasmid containing the GFP and antibiotic resistance gene?

  • @eduardoquiroga5783
    @eduardoquiroga5783 5 лет назад +1

    >.> we just basically saw someone fluff up their shit, in the end, realizing you can't microwave it.

  • @AdityaSharma-ox5jy
    @AdityaSharma-ox5jy 3 года назад +1

    What is your full time job?

    • @Jimiticus
      @Jimiticus  3 года назад +3

      I'm a technologist for a film/tv program at a college

  • @karlmoreno4109
    @karlmoreno4109 4 года назад

    Can you make a video on how to make the incubator you made?

  • @Unholygamewinner
    @Unholygamewinner 3 года назад +2

    Subbed in the hope a new vid will pop up in my sub box someday!😭❤️

  • @alexanderher7692
    @alexanderher7692 5 лет назад

    Could this to be done to make CBD at home, so I dont have to get ripped off from pharma companies

  • @hyperambience6710
    @hyperambience6710 3 года назад

    Remember: 'Discard the tip!'

  • @MrObble
    @MrObble 4 года назад +1

    I just join this channel's patreon, seems like a dead channel , I wish i was more knowledgeable about this stuff. my gut instinct is to support this channel. ( loved the space video ) which for some stange reason was already liked by me but i dont remember seeing it before. I really wish i knew more about biology

    • @Jimiticus
      @Jimiticus  4 года назад +1

      Thank you! I don't have any immediate plans but maybe one day I'll make another video

    • @Mantogods
      @Mantogods 4 года назад

      @@Jimiticus brah

  • @assidiq178
    @assidiq178 5 лет назад +1

    oh god this video is exactly what i have been done on my university lmao

    • @Jimiticus
      @Jimiticus  5 лет назад +2

      I hope you had better results than me!

    • @assidiq178
      @assidiq178 5 лет назад +1

      @@Jimiticus well no
      I already done like these and our work group done poorly lmao
      I even understand the inside joke on this video
      Also great vid man!

    • @Jimiticus
      @Jimiticus  5 лет назад +2

      @@assidiq178 Thank you!

  • @chrisyanover1777
    @chrisyanover1777 4 года назад

    How do you test for and adjust for any mutations?

  • @Chatisthisrealquestionmark
    @Chatisthisrealquestionmark 2 года назад +1

    kids today.. aw jeez i want to create some fluorescent yeast using DNA manipulation.

  • @ollieone051
    @ollieone051 4 года назад

    Can I make glowing beer with this?

  • @stijnnaets6873
    @stijnnaets6873 2 года назад

    Why microwave?

  • @Retipster
    @Retipster 5 лет назад

    Do you animate these videos yourself or are you working with a professional animator?

    • @Jimiticus
      @Jimiticus  5 лет назад

      Myself

    • @Retipster
      @Retipster 5 лет назад

      @@Jimiticus - they've very impressive. Nicely done. Have you ever done this kind of animation work for other people?

    • @Jimiticus
      @Jimiticus  5 лет назад

      No not really

  • @zEliazar
    @zEliazar 3 года назад

    RIP :(

  • @Studiepi
    @Studiepi 5 лет назад

    Will u every upload agian I really miss ur videos

    • @Jimiticus
      @Jimiticus  5 лет назад

      Possibly, but no immediate plans

    • @Studiepi
      @Studiepi 5 лет назад

      @@Jimiticus okay

  • @johnvictors8673
    @johnvictors8673 5 лет назад

    isnt e,coli dangerous bactery can cause serious ilness?

    • @Jimiticus
      @Jimiticus  5 лет назад

      Only certain strains of ecoli.

  • @iosui8109
    @iosui8109 6 лет назад

    Hello, are there any books you would recommend me reading? The topic doesn't really matter. :)

    • @Jimiticus
      @Jimiticus  6 лет назад +1

      About science in general?

    • @iosui8109
      @iosui8109 6 лет назад

      Jimiticus yeah

    • @Jimiticus
      @Jimiticus  6 лет назад

      A good place to start might be this popular book - A Short History of Nearly Everything by Bill Bryson - www.amazon.ca/Really-Short-History-Nearly-Everything/dp/0385666861/ref=pd_lpo_sbs_14_t_1?_encoding=UTF8&psc=1&refRID=6E4STYNA8B17HZ7RVAF8

  • @AdityaSharma-ox5jy
    @AdityaSharma-ox5jy 2 года назад +1

    There is this really good science animated video I'm making (almost like your kind). If I give 25% of the potential earnings from that video to you and you think it's a good video, will you make a RUclips post that mentions my video? The post can be similar to an Instagram post, something RUclips now allows.

  • @zapthebird8141
    @zapthebird8141 5 лет назад

    Hello uncle
    🖐

  • @Spirit_Circle
    @Spirit_Circle 6 лет назад +2

    How long till I can engineer myself?

    • @Jimiticus
      @Jimiticus  6 лет назад +3

      I guess it depends on what you want changed! I think the first treatment using Crispr has been approved for some sort of auditory genetic disorder. So it might be awhile!

  • @kadourkadouri3505
    @kadourkadouri3505 3 года назад

    That music is very unnecessary !

  • @Amsl420
    @Amsl420 4 года назад +1

    Wtf please make some vids about this kits and can i change the dna of rats with these kits?

  • @JaviDC93
    @JaviDC93 5 лет назад

    spanish!!!! por favor!!

  • @GPickle32
    @GPickle32 5 лет назад

    Ahh I see you still respond to comments

    • @Jimiticus
      @Jimiticus  5 лет назад +1

      Occassionally yes...

  • @tiberiusstaicu7853
    @tiberiusstaicu7853 3 года назад

    imagine glow in the dark bread

  • @Shkvarka
    @Shkvarka 5 лет назад

    God bless you and your channel!! haha, just kidding, there is no God=) Awesome channel and great work, thank you very much!!!!!

  • @santii9305
    @santii9305 6 лет назад

    Are you Chubbyemu?

  • @erlfram
    @erlfram 6 лет назад

    Don't do this. It's very cumbersome, and the result didn't taste any good at all! :/

  • @pablo-kx3to
    @pablo-kx3to Год назад

    1:02 my tripofobia

  • @7Earthsky
    @7Earthsky 4 года назад

    Should really explain the experiments one at a time....The video was a confused mess.

  • @telotawa
    @telotawa 2 года назад

    odin sucks tho

  • @syyylvo
    @syyylvo 4 года назад

    you shouldn't publicize this stuff online. not everyone should be performing such procedures