What Are: Knockout Mice?
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- Опубликовано: 17 дек 2020
- From the Creation of the First Knockout Mouse Scientists have come very far; with CRISPR technologies being the main method used by genetic knockout scientists today, the traditional esc based technology explained in this video is something of the past, mainly due to the length of time these projects take to complete (8-12 months).
Please note that any percentages mentioned in this video should be taken with a grain of salt as they are not exact values. For example, the targeting efficiency of vectors vary greatly not only from loci to loci, but also from vectors to vectors. 10% is just a rough average targeting efficiency in our hands.
More information about our knockout mice could be found at:
www.cyagen.com/us/en/service/...
Cyagen is the worlds leading provider of genetically modified mice and rats. We have produced over 50,000 projects to date. We also specialize in embryonic stem cells and the special media used in this video for research purposes.
You can find all our products at www.cyagen.com
Website: www.cyagen.com/us/en/
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More information about our knockout mice could be found at:
www.cyagen.com/us/en/service/crispr-based-genome-editing-knockout-mice.html
Explained 100x better than my professor! Awesome video.
Thanks, we are happy it helped better explain knockout mice for you!☺
Thank you ! very helpful and informative
quick and easy explanation, thanks.
Thank you very much! ❤️
Perfect explanation
Very helpful. Thanks
Thank you!
thank you!
Hi Cyagen I have a quick question: how are we putting an antibiotic resistance gene into a eukaryotic cell? The stem cells from the blastocyst are from a eukaryotic organism.. so I'd assume that antibiotics wouldn't be able to kill these cells anyways? Please let me know if I'm misunderstanding something. Thanks!!
I was actually wondering the same thing, why do we add neomycin, if eukaryotic cells don’t get affected by antibiotics.
Is there anyone here who can explain to me why do we always obtain heterozygous cells after homologous recombination?
Bcos AA *aa =Aa crossing over
Thats helpful.
If we do PCR of all ESC, how are we left with cells to be implanted in the new mice blastocyst?
Typically, one or more subcultures of cells can be made for analysis and selection of the correctly modified cells.
Thanks@@Cyagen
Pls send notes of this
Thanks for your interest in our knockout mice. More information could be found at: www.cyagen.com/us/en/service/crispr-based-genome-editing-knockout-mice.html
Just wondering, are there technologies or drugs that can knock out Genes by simply giving oral drugs while being pregnant?
Nobody:
Pfizer: "Let's knock out humanity!" :D
Yeah, after I got the vaccine I stopped existing.
@@lucasjones3338 You will never have children.
@@Name-zd5fq pretty sure thousands of vaccinated people have already had children lol
@@KlearChristal LOL...let's see how long you'll laugh. :D