The Basics of Transgenic Mice: Pronuclear Injection - How It Work and What Scientists Use It For

Transgenic mice can be important tools for researching human genes which can be added to the mouse genome with a simple pronuclear injection into the male pronucleus. Watch the video to find out how this works and how scientists use them.
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music:
Inspired Kevin MacLeod (incompetech.com)
Licensed under Creative Commons: By Attribution 3.0 License
creativecommons.org/licenses/b...
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[@mazdakasadian9100]

Great Explanation! Thanks for the effort!

[@priyakamboj5850]

Thanku.for giving such a valuable content....👍

[@user-xe5wo6tn8u]

I couldn't be me more thankful to you for these videos. Amazing. I have been studying a paper for a PhD interview and I couldn't understand a thing. But thanks to your videos, now I do. Thank you again. Keep up the good Work, Please.

[@Arifkisana]

Very Informative and brief. Can you please give details to prepare Foster mother by inducing progestron harmon. Timings, dose and other details.

[@alexanderdiederichs7332]

Thank you, for your nice explanation!

[@zabranjenakojasko6833]

@@Cyagen please don't I will have to report you

[@omarkayali4482]

Regarding the step 3 of transgenesis, the procedure through which the transgene is introduced is called TRANSFECTION. Transformation is done autonomously by micro-organisms ( as demonstrated by the Griffith experiment).
Nice video and well done.

[@zabranjenakojasko6833]

Did you test it on yourself ??

[@ellios5734]

Thank u so much for explaining and the animation

[@zabranjenakojasko6833]

Are you volunteering?? It's just an injection 🤣🤣🤣🤣

[@ellios5734]

@@zabranjenakojasko6833 IDK what u mean they rlly explained it good

[@MrsLeelon]

Very well made Explainty! It's a shame your Channel and Videos don't get as much attention as they deserve.
Keep going, your videos are great.

[@themoralshow902]

Thank you❤️😊
From Sri Lanka 🇱🇰😍

[@sabrinar.5372]

Could i use this technique to over-express a gene already in a mouse? If not, what other methods can i use for this?

[@josiahfresnel9217]

someone over-expressed my balls genes; they are HUGE

[@Cyagen]

Transgenic insertion of a plasmid containing the mouse gene of interest may be performed to generate an overexpression model, but this has an unknown number of gene insertions. For a single-copy insertion overexpression model, we can perform PiggyBac-on-BAC transgenesis.

[@056vatsalapandey3]

plasmids occur in prokaryotes. can recombinant plasmids be inserted into mice?

[@CataclysmicPranks]

Yes

[@achintkumar1022]

in the 3rd step of transgenesis you insert gene of intrest with vector (plasmid) , why cant we just directly inject naked gene into the male pronucleus

[@ellios5734]

Me: Okay so let's search to find the answer it's 21st century after all
The video: the exact mechanism has not been determined
Me: 👁️👄👁️