The Cre-loxP Technique (Transgenic Mice)

Cre-loxP system is a highly efficient system to create transgenic mice. It relies on the ability of Cre recombinase to bind and recombine sequences flanked by loxP sites. Using Cre-loxP system, it is possible to study gene function by either activating or inactivating a gene in a tissue-specific manner. The gene can also be inactivated if an inducible promoter is used, e.g. Doxycycline or Tetracycline-based promoters. The system has been used to create mouse models of several human diseases and has led to a better molecular understanding of many diseases.

[@yoolim1206]

After watching a bunch of videos explaining about Cre Lox P I eventually understood the mechanism. The best explanation!! Thank you soo much !!

[@SpartanTutorials]

Thank you Yoolim. Glad you found the explanation useful.

[@robindurand9384]

That's a great reminder of the Cre-LoxP system! Thank you very much

[@SpartanTutorials]

Welcome Robin. Glad you liked it.

[@IvanovBR]

Excellent class! Helped me to explain better the technique to my students!

[@SpartanTutorials]

Thanks! Glad to know it was useful.

[@ameliajanebrown108]

Thank you so much, I was never able to understand this before!

[@SpartanTutorials]

My pleasure Amelia.

[@ukaszniznik870]

Thank you so much Sir, Your video helped me to understand practical usage of CreLoxP system better

[@SpartanTutorials]

My pleasure Lukasz.

[@zacharybunch3988]

Fantastic video! Gave you a thumbs up!!!!

[@SpartanTutorials]

Thanks Zachary!

[@amnabibi8536]

Very well explained

[@SpartanTutorials]

Thank you!

[@mohammadsikander4324]

thank you so much

[@SpartanTutorials]

Most welcome Mohammad.

[@user-lp5qe1tv7d]

very informative thanks

[@SpartanTutorials]

Thank you!

[@ravikantyadav9464]

Amazing sir ☺️

[@SpartanTutorials]

Thank you. 🙂

[@israaomar9487]

Thank you so much Dr

[@SpartanTutorials]

Most welcome Omar (I’m guessing that’s your first name).

[@israaomar9487]

@@SpartanTutorials it is Israa i am a PhD student in molecular medicine, i find this lecture is very useful. Thank you so much Doctor. U literally saved me,God bless you. Thumbs up

[@SpartanTutorials]

@@israaomar9487 Got it Israa. Most welcome! Thanks for your kind words!

[@SpartanTutorials]

@@israaomar9487 My pleasure Israa. I think you'll also like our FREE app which contains a lot of books and videos (all free) for students of medicine and biology. Download it here: bit.ly/3ufso54 All the best! - Rupinder.

[@sharanchinniah8925]

amazing video

[@SpartanTutorials]

Thanks Sharan.

[@farzadinam8339]

thank you for the input Sharan!

[@tusarbehera6359]

Lots of information ❤tq

[@SpartanTutorials]

Welcome 😊

[@parisaap6921]

Hi,Thank you so much for this amazing video. I have a project about this topic is there any way to help me about it? I really need help to do it .I am so confused .
If it is possible I really appreciated it .

[@SpartanTutorials]

Sure, You can contact me by email at [e-mail]. Good luck!

[@Bio-Med]

Excellent sir what software uh use to deliver lectures?

[@SpartanTutorials]

Thanks! I use OBS Studio, an excellent free software.

[@mohammadsikander4324]

i subscribed with smile

[@SpartanTutorials]

Thanks for the subscribe Mohammad. Hope you find the videos here useful. Let me know if there are any topics you want explained, I’m looking for suggestions for my next videos.

[@drnima6160]

it was perfect

[@SpartanTutorials]

Thank you!

[@Odiapakhi]

A doubt: Here the Alb-Cre is in a hemizygous state and also in a different chromosome?

[@SpartanTutorials]

Gayatree, the Alb-Cre can be in a hemizygous or homozygous state depending upon researcher's experimental setup, and yes, it is on a different chromosome. Since a variety of these mice are available carrying Cre-lox construct on different chromosomes, knockouts can be easily generated for genes present on different chromosomes.

[@mankuzhy]

Since hemizygous mice have a wildtype chromosome present, using hemizygous instead of homozygous Cre genotypes might minimize unintended consequences of random transgene insertion. (Courtesy: From Jax site)

[@MrCharlesdick]

Isn't there a way to design a Cre-Lox system that copies itself onto the homologous chromosome so that every offspring between a transgenic individual and a wild-type individual will be a transgenic individual? A "gene drive"?