The 3D Organization of our Genome: now corrected on

Title: The 3D Organization of our Genome
This video was about Genome folding in the 3D space of the cell nucleus. The description contained an extensive series of further readings and video links to get more insight in this fascinating topic.
BUT - after posting we realized that, because of a flip in image layers, DNA got left-handed and wraps in a right handed manner to form nucleosomes. This is the reverse to what should be: DNA is actually right-handed and wrapped in a left-handed superhelix to form nucleosomes.
We therefore posted the corrected video on • The 3D Organization of... , please go there to watch the correct version and find related information.
Apologies everybody and enjoy chromosome biology !

[@rajivaraman5726]

Very interesting model of chromosome organization. The concept of nucleosome clutches of variable number of them coming together and then organizing into chromatiin nano domains (CNDs) is a new thought which further highlights dynamicity of chromatin in interphase. A good model to work upon.

[@dianabuzas2722]

Is there a quick reference for how one detects nucleosome clutches experimentally?

[@cavallilabvideos8738]

Yes, nucleosome clutches were first observed by Maria Aurelia Ricci and colleagues in 2015, using the incorporation of a fluorescent histone into the chromatin fiber and its observation with Stochastic Optical Reconstruction Microscopy (STORM, see link 1- below), an advanced fluorescence microsopy method that provides extremely high spatial resolution. These small nucleosome aggregates were confirmed by Ou and colleagues, using ChromEMT, which combines "multitilt electron microscopy tomography" with a labeling method to selectively enhance the DNA contrast (again, advanced microscopy methodology, see link 2- below). These nucleosome aggregates represent a disordered 5- to 24-nm-diameter curvilinear chain that can be further packed inside interphase nuclei:
1- www.cell.com/action/showPdf?p...
2- doi.org/10.1126/science.aag0025