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Cavalli lab videos
Франция
Добавлен 5 авг 2020
This is the Cavalli lab video channel. It features video abstracts describing scientific discoveries of our lab, which relate to our publications.
For a short intro on our interest, go to ruclips.net/video/5PEp1Ia4ROc/видео.html. This video is in French, but you can add subtitles. For English subtitles, click the "cc" button on the bottom right, then click on the "settings" button on the bottom right, the select "subtitles", "Auto-translate", English.
For more information of our lab, go to: www.igh.cnrs.fr/en/research/departments/genome-dynamics/21-chromatin-and-cell-biology
For other media profiles of Giacomo Cavalli:
Twitter: giacomo_cavalli - @giacomo_cavalli
Google Scholar: scholar.google.com/citations?user=5oWMD9EAAAAJ&hl=en
For a short intro on our interest, go to ruclips.net/video/5PEp1Ia4ROc/видео.html. This video is in French, but you can add subtitles. For English subtitles, click the "cc" button on the bottom right, then click on the "settings" button on the bottom right, the select "subtitles", "Auto-translate", English.
For more information of our lab, go to: www.igh.cnrs.fr/en/research/departments/genome-dynamics/21-chromatin-and-cell-biology
For other media profiles of Giacomo Cavalli:
Twitter: giacomo_cavalli - @giacomo_cavalli
Google Scholar: scholar.google.com/citations?user=5oWMD9EAAAAJ&hl=en
3D Organization Unseres Genoms Deutsch
Schlüsselwörter: Genom, Chromosom, Chromatin, 3D-Genom, Epigenetik
Synopsis: Dieses Video rekapituliert unser aktuelles Verständnis der Genomorganisation im dreidimensionalen Raum des Zellkerns, beginnend mit Nukleosomen, die 146 Basenpaare der DNA umhüllen, bis hin zu ganzen Chromosomengebieten, die Hunderte von Millionen Basen Paare von DNA-Sequenzen enthalten können. Zwischen diesen beiden Extremen liegen die hierarchische Faltung der Chromatinfaser in „Nukleosom Clutches“, „Chromatin-Nanodomänen oder CNDs“, „Topologisch assoziierende Domänen oder TADs“ - einschließlich ihres Bildungsmechanismus durch Schleifenextrusion - und die „A und B-Kompartimente“ vorgestellt. Nachfolgend finden S...
Synopsis: Dieses Video rekapituliert unser aktuelles Verständnis der Genomorganisation im dreidimensionalen Raum des Zellkerns, beginnend mit Nukleosomen, die 146 Basenpaare der DNA umhüllen, bis hin zu ganzen Chromosomengebieten, die Hunderte von Millionen Basen Paare von DNA-Sequenzen enthalten können. Zwischen diesen beiden Extremen liegen die hierarchische Faltung der Chromatinfaser in „Nukleosom Clutches“, „Chromatin-Nanodomänen oder CNDs“, „Topologisch assoziierende Domänen oder TADs“ - einschließlich ihres Bildungsmechanismus durch Schleifenextrusion - und die „A und B-Kompartimente“ vorgestellt. Nachfolgend finden S...
Просмотров: 565
Видео
Marc Marti Renom's Seminar on Technologies to study 3D Genome Folding
Просмотров 7162 года назад
This video features a video conference by Marc Marti-Renom, talking about imaging, genomics and computational methods to study three-dimensional chromosome folding and genome function. The conference was held on December 1st, 2021 for the Institute of Human Genetics of Montpellier ( IGH_MTP and www.igh.cnrs.fr/en) For more information about Marc's lab work, check out www.crg.eu/marc_...
The 3D Organization of Our Genome
Просмотров 60 тыс.2 года назад
Keywords: Genome, chromosome, chromatin, 3D Genome, Epigenetics Synopsis: This video recapitulates our current understanding of genome organization in the three-dimensional space of the cell nucleus, starting from nucleosomes, which wrap 146 base pairs of DNA, all the way to entire chromosome territories that may contain hundreds of million base pairs of DNA sequence. In-between these two extre...
The 3D Organization of our Genome: now corrected on https://www.youtube.com/watch?v=Pl44JjA--2k
Просмотров 7 тыс.2 года назад
Title: The 3D Organization of our Genome This video was about Genome folding in the 3D space of the cell nucleus. The description contained an extensive series of further readings and video links to get more insight in this fascinating topic. BUT - after posting we realized that, because of a flip in image layers, DNA got left-handed and wraps in a right handed manner to form nucleosomes. This ...
Epigenetics and Polycomb Proteins Snippet5: roles in Cancer
Просмотров 8434 года назад
Part 5 on a video series on the action of Polycomb proteins in epigenetic regulation of gene expression in cell differentiation and cancer. See article at: onlinelibrary.wiley.com/doi/full/10.1002/bies.201800222. Video by InMotionScience.
Epigenetics and Polycomb Proteins Snippet4: roles in neuronal differentiation
Просмотров 4624 года назад
Part 4 on a video series on the action of Polycomb proteins in epigenetic regulation of gene expression in cell differentiation and cancer. See article at: onlinelibrary.wiley.com/doi/full/10.1002/bies.201800222. Video by InMotionScience.
3D Chromosome Folding Features During Neural Cell Differentiation
Просмотров 7394 года назад
This video describes the main features of 3D genome folding during in vitro and in vivo neural differentiation during mouse embryonic development. It accompanies a scientific paper (Bonev et al., Cell 2017) that can be found here: www.cell.com/cell/fulltext/S0092-8674(17)31137-6. The video was made by InMotionScience and was originally published here: ruclips.net/video/JYDrtCuRKVM/видео.html
The role 3D genome folding and Polycomb proteins in development
Просмотров 4574 года назад
Video abstract accompanying a 2018 scientific publication that can be found here: www.cell.com/molecular-cell/fulltext/S1097-2765(18)30407-6?innerTabvideo-abstract_mmc5= The video is from InMotionScience and was originally published here: ruclips.net/video/HFtGxda3I8E/видео.html
Epigenetics and Polycomb Proteins Snippet1: Polycomb complexes
Просмотров 3,4 тыс.4 года назад
Part 1 of a video series on the action of Polycomb proteins in epigenetic regulation of gene expression in cell differentiation and cancer. See article at: onlinelibrary.wiley.com/doi/full/10.1002/bies.201800222. Video by InMotionScience.
Epigenetics and Polycomb Proteins Snippet2: Roles in transcriptional repression
Просмотров 8834 года назад
Part 2 of a video series on the action of Polycomb proteins in epigenetic regulation of gene expression in cell differentiation and cancer. See article at: onlinelibrary.wiley.com/doi/full/10.1002/bies.201800222. Video by InMotionScience.
Epigenetics and Polycomb Proteins Snippet3: roles in stem cells and differentiation
Просмотров 5834 года назад
Part 3 of a video series on the action of Polycomb proteins in epigenetic regulation of gene expression in cell differentiation and cancer. See article at: onlinelibrary.wiley.com/doi/full/10.1002/bies.201800222. Video by InMotionScience.
Polycomb group proteins in Drosophila embryogenesis
Просмотров 1,6 тыс.4 года назад
Video abstract describing the role of Epigenetic components of the Polycomb group proteins in early Drosophila development. This video accompanies a scientific publication that can be found here: www.nature.com/articles/s41467-018-05945-4 The video was made by InMotionScience and was originally published here: ruclips.net/video/3UufJY5J7jk/видео.html
Transgenerational Epigenetics
Просмотров 2 тыс.4 года назад
Snippet from a larger video overview of Epigenetics, since the beginning to our days, and of its implications for ecology, evolution and human disease. Extract from a memorial review celebrating the 150th anniversary of Nature Journal. The review series can be found at www.nature.com/collections/dgabdjddji and the specific review is found here: www.nature.com/articles/s41586-019-1411-0
The relation between Epigenetics and DNA sequence variation
Просмотров 2334 года назад
Snippet from a larger video tracing an overview of Epigenetics since the beginning to our days, and its implications for ecology, evolution and human disease. Extract from a memorial review celebrating the 150th anniversary of Nature Journal. The review series can be found at www.nature.com/collections/dgabdjddji and the specific review is found here: www.nature.com/articles/s41586-019-1411-0
Epigenetics and the Environment
Просмотров 3874 года назад
Snippet from a larger video overview of Epigenetics, since the beginning to our days, and of its implications for ecology, evolution and human disease. Extract from a memorial review celebrating the 150th anniversary of Nature Journal. The review series can be found at www.nature.com/collections/dgabdjddji and the specific review is found here: www.nature.com/articles/s41586-019-1411-0
So it is just a big mess.
I have one doubt i.e is it helpful for the recombinant DNA technology (the new paradigm) can any one tell me plzz..,
Yes it is. Indeed, a frequent observation when inserting DNA sequences into the genome in order to express proteins is that the DNA can not be expressed, or is initially expressed but mRNA expression becomes silenced over time. This is frequently due to the packaging of chromosomal DNA into condensed structures. Therefore, understanding the 3D folding of chromosomes allows one to improve DNA technologies in order to enable better and more stable expression of desired genes.
Who did put information in DNA? Sequences are body of intelligent information. Junk DNA? But there is no junk phenotype
Absolutely fantastic, thank you for making this.
Glad you enjoyed it! Hope to put out another one by the end of Summer !
7176D
it all sounds theoretical... gotta look up latest info
Thank you very much!!!
This aged extremely well. Your lab is due for another round of videos. Your animation style is fantastic and great for classrooms. Do more
Thanks a lot, we'll go for it nex year! All the best
Well that was a lot to unpack 🥁😂
Great things never come from the comfort zone.. We never stop innovation.!
❤❤
Very well explined, I am very clear about the concept of genome now.
Thanks for posting this, it helps clarify the scales involved. One question I haven't been able to find an answer to: Is it known whether the larger chromatin structures are anchored in place by actin filaments?
Excellent video! Clear/straightforward/concise visualization of these different levels of organization. Beautifully done ...thank you for sharing! One thing I didn't understand though, (if anybody can enlighten this layman), ...the highest level noted, "chromosone territory", is said to correspond to entire chromosones, but the visual shows what I presume are those seperate entire chromosones, (represented as different colors), as amorphus blobs smushed against each other. Why do we not see the typical seperate "X" shapes of each seperate chromosone commonly seen under a microscope? Am I misinterpreting something?
Very good question! The X shapes are a special, highly condensed state of chromosomes which is only present as the cells are diving, in the process known as mitosis. This "X"' state is optimum for neatly segregating chromosomes into daughter cells without mistakes, but it is so condensed that it is incompatible with other functions such as transcription into RNA or duplication of the DNA that is required before the cells can divide. During the remaining time of the cell life outside mitosis, when the cell is functioning, producing proteins and metabolites that maintain our body function, chromosomes partially decondense and they are no longer visible with this X shape but take the form of the pseudo globular chromosome territories depicted in the video. You can take a look at the education video: ruclips.net/video/gbSIBhFwQ4s/видео.html. The first 30 seconds show folding into nucleosomes and are correct. From 30 seconds to 1minute 15 seconds they model the folding of nucleosomes into chromatin fibers, and this is the part that needed to be corrected, which we did with our video. After 1 minute 15 seconds you see the X-shaped chromosomes in mitosis and then you see how they decondense under the microscope in a time-lapse video of a cell mitosis. Hope this helps!
@@cavallilabvideos8738 Thank you ...extremely helpful! Your explanation makes it all very clear, and I see that "X" state is indeed noted as very temporary/mitosis only in the other video you noted. Fascinating! These 3d visualizations are such an incredibly wonderful/powerful clarification tool!! So many aspects/details/contextual elements come to light that otherwise would be hazy or out of mind. (Though I suppose there are also numerous secondary aspects that must still be speculative in such inherently comprehensive/literal/thorough/detailed representations, (such as the particular motions for example perhaps). Regardless ...much better to have reasonable speculative placeholder secondary aspects to fill out a full picture.) Really appreciate what you do and share!!
Very useful. thank you
visualization helps a lot to understand a concept!! thanks a lot!
Glad it was helpful and all the best!
Is this animation based on actual microscopic or other images/ footage or just a theory?
Dear Nori, you will find in the discussion detailed information and further readings on many data that converge toward the interpretation that is showed in the video. Nevertheless, putting together experimental evidence on subjects of sizes that are over a million fold smaller than a centimeter (1-10 nanometer) is always a difficult and uncertain challenge and probably we will have to change several points as the knowledge progresses in the coming years. Stay tuned ...
@@cavallilabvideos8738 Thank you!
Fantastic video! Thanks!😊
Thank you Tina, I'm glad it's useful! All the best, giacomo
Excellent 👍
The genes for the heavy and light chains of immunoglobulin are in chromosome 2, 14 and 22. I was wondering if these chromosomes are neighboring each other in the nucleus and specifically in the b-cell. In this nice animation I see that the chromosomes are colored. Is there a map between the colors and the chromosomes?
Great question and I can not answer but the 3D organization of the B-cell genome was studied in this paper: www.nature.com/articles/s41467-020-20849-y. You might contact the corresponding authors José Martine-Subero at imartins@clinic.cat or Marc Marti-Renom @ martirenom@cnag.crg.eu for more infos!
Great work. Thanks
Awesome thank you!!! More please!!!
Beautiful and crisp.
is there a particular histone acetylation mark that affects PRC repression?
Yes, H3K27Acetylation will prevent PRC2 to deposit H3K27 methylation by direct competition and so H3K27Ac blocks silencing. Furthermore, H3K36 di- or trimethylation inhibits PRC2. see article DOI: 10.7554/eLife.61964 for an explanation of the mechanism.
Who did the animation?
It is a company called Arkitek Scientific, www.arkitek.com/ ! Excellent people, we had lots of discussions with them in order to get the concepts straight and then to refine them in order to be precise and improve the rendering, they were responsive and efficient throughout the process !
Superb
India education better than France education because India ancient time Sushrut teaching the world how to teach anybody but your explanation is very nice
Hii I am from India
Very clean representation and narration. 10/10. It's also very fascinating and a privilege to study.
We are glad to hear that it was useful!
Thank you for posting this. I will spread this video around, this is absolutely fascinating to anyone in the cell bio world.
Is there a quick reference for how one detects nucleosome clutches experimentally?
Yes, nucleosome clutches were first observed by Maria Aurelia Ricci and colleagues in 2015, using the incorporation of a fluorescent histone into the chromatin fiber and its observation with Stochastic Optical Reconstruction Microscopy (STORM, see link 1- below), an advanced fluorescence microsopy method that provides extremely high spatial resolution. These small nucleosome aggregates were confirmed by Ou and colleagues, using ChromEMT, which combines "multitilt electron microscopy tomography" with a labeling method to selectively enhance the DNA contrast (again, advanced microscopy methodology, see link 2- below). These nucleosome aggregates represent a disordered 5- to 24-nm-diameter curvilinear chain that can be further packed inside interphase nuclei: 1- www.cell.com/action/showPdf?p... 2- doi.org/10.1126/science.aag0025
Extraordinaire. Magnifique . Merci pour le partage.
Great video! Thank you!
Very interesting model of chromosome organization. The concept of nucleosome clutches of variable number of them coming together and then organizing into chromatiin nano domains (CNDs) is a new thought which further highlights dynamicity of chromatin in interphase. A good model to work upon.
Thank you