Thank you!! There's so many videos and advice out there trying to find shortcuts to circumvent proper technique! It's amazing the amount of success you can have with proper technique, even with limited equipment!!
I think you have a culture/spawn lab and a bag inoc lab which is a great great way to make quality spawn. A few humble suggestions: 1) take your own advice and buy a bacteriacinerator before you light yourself or the lab up but good someday lol and keep it clean/covered when not in use - surely to heavens you can afford it now (and deserve it), 2) cut your parafilm horizontally so it doesn't break as easily or stress break at a later date as easily (it has a "grain" that should be considered when stretching it, try it and see), 3) get a list of heat stable antibiotics from google (however, tilting the plate will eliminate bacteria without antibiotics, bacteria can't run up hill like mycelium), 4) wet does not mean the alcohol is still there and alcohol will not kill many contams and takes a while to kill contams (more than a few seconds), 5) a wire rack will lift your plates above the vortices near the bottom of your flowhood (the air from the filter is not level with the table and has a 1/2" gap that causes the air to spin) - try a smoker and you'll see what I am talking about. But hey, if only 1/1000 are contaminating you're doing better than me and I'm running 100-500 plates at a time and about a 100mt a year of all wild taken spawn. Love that you are drilling home that a slow plate has something invisible to the human eye that is causing issues (usually a white contam from other mycelium) and should be discarded. Keep teaching and helping the industry grow, please. Love the videos.
Antibiotics in farming ( any farming) needs to be strictly regulated. Probably why they're calling for vet scripts for all farming Antibiotics now. For the life of me I don't get why the farming industry still thinks just throwing Antibiotics at everything is going to work. It never works & only continues to create super germs.
This is great info! I watch these videos with a notebook now…. 👍 I’m still confused about master, G1, G2. If you make G1 from the master culture, wouldn’t you run out of master? If you make a slant from master would that be a G1? Ugh. Anyway. Love the videos. I’m almost through all of them. My fridge is slowly filling with plates…. Thank you!
Damn...If I move my plate around like you do, my transfer would have slid to another country....good tip about the red sharpie "lefty", thanks. I've noticed your video's are now "Mirrored" ...meaning all the writing looks backward like looking in a mirror. Probably a camera, or video editor setting flipping your pictures to mirror mode...I noticed a few back with the righting on your shirt.....so maybe your not a "lefty"
Hahaa.... I very well remember my first time transferring. Trembling & shaking. Great video's, I learned a lot of listing to and watching you. Thanks a lot! There is not one day I do not enjoy watching mycelium or mushrooms grow. It remains impressive. I am also one of the not so well organized types.
@@jordanmercier3616 I very well was the one who did it..esp if was during the civil war..I'm almost 200 years old now..I hope it healed without any complications, as I try to keep it sterile as possible
I just freshly tested the red sharpie on a plate and it literally washed off within seconds of spraying 70% iso…. Gonna test again by letting it set for a few days then spray 70%iso again to see if it stays..
You should check out the blade brand Feather. I’ve found the cheap”” amazon ones when heated multiples times will get little pieces of metal that will go into your agar when you cool it down with your receiving dish. This happened for me just after heating the blades several times. Feather brand I can heat up and use so many times until the blade starts warping. A box of 100 costs about $25-$30 but they are soooo much better then the $8 for 100 on amazon. Feather is the same brand that Fungiperfect sells :)
Hey T.R, what is your antibiotic agar recipe, i.e., how much gentamicin per liter of agar you use? Also, i have a culture that is constantly being attacked by bacteria, im thinking its growing along with it, i cant seem to clean it off. Im assuming a/b agar would maybe solve this, right?
Bahahahaha… at home, my right brain is in charge - I’m a messy, disheveled artist. At work, in surgery, “anal” doesn’t begin to cover describing my left brain! It was well known that one may leave my surgical room with bruising if they touched or moved ANY of my instruments/drugs/specimens…!
Yes. A culture that grows with no contamination has no contamination...irregardless if it's made in mom's (or dad's) basement, or not. More like "The quality of the culture, is the quality of the culture"
Just seeing this comment. Sorry for the late reply......"A culture that grows with no contamination, has no contamination" is true. The flip side to this is, you don't see microscopic particles. That said, a plate (or grain) can be contaminated and it will appear clean, yet the second it gets transferred it will contaminate everything in its path. So a more accurate statement would be "a culture with no visible contam, doesn't necessarily mean it isn't contaminated." If a person doesn't believe this, they 1. Haven't been making cultures and spawn long enough or to a scale that they would see this 2. Dont know anyone who has made enough spawn/cultures or has an operation at a commercial scale that they can learn from. At the end of the day, most cultivation stuff is general math........ it's spawn and culture making that can introduce you to quantum physics. Anyone who says spawn/ culture work is easy should 100% disregarded. All the largest spawn makers in the world have a set contamination rate that they operate with. Yes, multi million dollar facilities with all the top technology and no joke phds running their labs, still have contaminated spawn at a set % every week. This screams, it's harder than the newbs on Facebook are making it out to be.
Thank you for showing your technique! Very helpful!
HE'S BACK! I knew there was something worth getting on the computer for
Thank you!! There's so many videos and advice out there trying to find shortcuts to circumvent proper technique! It's amazing the amount of success you can have with proper technique, even with limited equipment!!
Have I mentioned how much I love your videos? Please keep them up.
Thanks, i appreciate it.
I think you have a culture/spawn lab and a bag inoc lab which is a great great way to make quality spawn. A few humble suggestions: 1) take your own advice and buy a bacteriacinerator before you light yourself or the lab up but good someday lol and keep it clean/covered when not in use - surely to heavens you can afford it now (and deserve it), 2) cut your parafilm horizontally so it doesn't break as easily or stress break at a later date as easily (it has a "grain" that should be considered when stretching it, try it and see), 3) get a list of heat stable antibiotics from google (however, tilting the plate will eliminate bacteria without antibiotics, bacteria can't run up hill like mycelium), 4) wet does not mean the alcohol is still there and alcohol will not kill many contams and takes a while to kill contams (more than a few seconds), 5) a wire rack will lift your plates above the vortices near the bottom of your flowhood (the air from the filter is not level with the table and has a 1/2" gap that causes the air to spin) - try a smoker and you'll see what I am talking about. But hey, if only 1/1000 are contaminating you're doing better than me and I'm running 100-500 plates at a time and about a 100mt a year of all wild taken spawn. Love that you are drilling home that a slow plate has something invisible to the human eye that is causing issues (usually a white contam from other mycelium) and should be discarded. Keep teaching and helping the industry grow, please. Love the videos.
Could you explain what you mean by tilting them? Good advice, ty
nothing wrong with alcohol lamps have been using one for 11 years ,and running a mushroom business
Antibiotics in farming ( any farming) needs to be strictly regulated. Probably why they're calling for vet scripts for all farming Antibiotics now. For the life of me I don't get why the farming industry still thinks just throwing Antibiotics at everything is going to work. It never works & only continues to create super germs.
I want to know more about anti-bac dishes. There are no mushroom growers with anti-bac vids.
nice one ,good couple of extra details thanks
I definitely need to start wiping my plates with alcohol after I pull off the parafilm
This is great info! I watch these videos with a notebook now…. 👍
I’m still confused about master, G1, G2. If you make G1 from the master culture, wouldn’t you run out of master? If you make a slant from master would that be a G1? Ugh. Anyway. Love the videos. I’m almost through all of them. My fridge is slowly filling with plates…. Thank you!
I have the exact same question! How do you manage generations and preserve the master? Which generation is actually used for colonising spawn?
Raising my hand also. So glad others asked. Thought it was me. I would love a little bit more tutorial on that. If you're taking requests. Thanks
“Look good”… when they ‘look good’, it means you’ve applied it evenly, thus more likely to have been done properly.
That, and Gent hasn’t been as overused like many of the other antibiotics… so the bad bugs aren’t as ‘accustomed’ to it.
BD Bard Parker blades are worth a bit extra $, as they won’t go dull as quickly as the cheaper off brands.
Thank you, Sir,.
Awesome!
Damn...If I move my plate around like you do, my transfer would have slid to another country....good tip about the red sharpie "lefty", thanks.
I've noticed your video's are now "Mirrored" ...meaning all the writing looks backward like looking in a mirror.
Probably a camera, or video editor setting flipping your pictures to mirror mode...I noticed a few back with the righting on your shirt.....so maybe your not a "lefty"
I just tested the red sharpie and it washed off within seconds of spraying 70%iso ….. kinda need to let TR know that he may have spread some missinfo
How are you protecting yourself from the alcohol fumes?
super human strength. That and i tell my lungs to not be pussies.
Thank you
Hahaa.... I very well remember my first time transferring. Trembling & shaking. Great video's, I learned a lot of listing to and watching you. Thanks a lot! There is not one day I do not enjoy watching mycelium or mushrooms grow. It remains impressive. I am also one of the not so well organized types.
I treat it like performing surgery
....puts the lotion in the basket.
How many surgeries have you performed??
@@jordanmercier3616 17, and 2 wisdom teeth extraditions..and during g the civil war I amputated 3 legs
@@killerkennyas what a coincidence, my leg was amputated as a child!
@@jordanmercier3616 I very well was the one who did it..esp if was during the civil war..I'm almost 200 years old now..I hope it healed without any complications, as I try to keep it sterile as possible
I just freshly tested the red sharpie on a plate and it literally washed off within seconds of spraying 70% iso…. Gonna test again by letting it set for a few days then spray 70%iso again to see if it stays..
Alcohol resistant cryo markers
Are you setting up for hunting season? It's almost deer and mushroom hunting time
You should check out the blade brand Feather. I’ve found the cheap”” amazon ones when heated multiples times will get little pieces of metal that will go into your agar when you cool it down with your receiving dish. This happened for me just after heating the blades several times. Feather brand I can heat up and use so many times until the blade starts warping. A box of 100 costs about $25-$30 but they are soooo much better then the $8 for 100 on amazon. Feather is the same brand that Fungiperfect sells :)
I'll have to look into those.
Well worth the extra money :) Paul staments uses them, must mean something!!!
Hi there amazing vid, what kind of flow hood do you have?
I built this flow hood with a 3' x 4' filter from Myers mushrooms.
Earth Angel Mushrooms
Hi. What do you make agar for mushrooms?
yes
Hey T.R, what is your antibiotic agar recipe, i.e., how much gentamicin per liter of agar you use? Also, i have a culture that is constantly being attacked by bacteria, im thinking its growing along with it, i cant seem to clean it off. Im assuming a/b agar would maybe solve this, right?
Bahahahaha… at home, my right brain is in charge - I’m a messy, disheveled artist.
At work, in surgery, “anal” doesn’t begin to cover describing my left brain! It was well known that one may leave my surgical room with bruising if they touched or moved ANY of my instruments/drugs/specimens…!
Keeit up!!!
I’m disorganized too ,I can relate, Can u recommend a good source for quality cultures?
Mycelium Emporium, Field & Forest.
Yes. A culture that grows with no contamination has no contamination...irregardless if it's made in mom's (or dad's) basement, or not.
More like "The quality of the culture, is the quality of the culture"
True, but the issue is that sometimes a dish looks clean until it goes to grain, and it turns out it isn't.
Just seeing this comment. Sorry for the late reply......"A culture that grows with no contamination, has no contamination" is true. The flip side to this is, you don't see microscopic particles. That said, a plate (or grain) can be contaminated and it will appear clean, yet the second it gets transferred it will contaminate everything in its path. So a more accurate statement would be "a culture with no visible contam, doesn't necessarily mean it isn't contaminated." If a person doesn't believe this, they 1. Haven't been making cultures and spawn long enough or to a scale that they would see this 2. Dont know anyone who has made enough spawn/cultures or has an operation at a commercial scale that they can learn from.
At the end of the day, most cultivation stuff is general math........ it's spawn and culture making that can introduce you to quantum physics. Anyone who says spawn/ culture work is easy should 100% disregarded. All the largest spawn makers in the world have a set contamination rate that they operate with. Yes, multi million dollar facilities with all the top technology and no joke phds running their labs, still have contaminated spawn at a set % every week. This screams, it's harder than the newbs on Facebook are making it out to be.
So how is the so called "contam" hiding if you can't see it on an agar plate?
@@dhaval4570
It all boils down to being uber conscious of your sterile technique and what you are/are not bringing into the lab with you.
Thanks. I learned sone good things here.
Can I use a candle flame?
That's what she said! 😉😂😁🍄
I'm glad someone caught that. I couldn't make it anymore obvious without someone getting offended.
@@earthangelmushrooms2118 keep up putn out the great content and thank you so much for the knowledge!
oorahdevildog
HUA