Cell Culture (Suspension Cell)

( www.abnova.com ) - Cell culture is the process by which cells are grown under controlled conditions. This video shows you how to culture suspension cells from cell thawing, cell maintenance, to cell freezing. More videos at Abnova www.abnova.com

[@patricklungu756]

Thank you for an educative video. Do have any video that shows cell stimulation of whole blood?

[@shivamarzi6092]

I am facing problems in growing Kasumi-6 cell line. I'm using RPMI 1640 with 20% FBS and then directly do the flask I'm adding 2ng/ml GM-CSF, l-glutamine and antibiotics. But they not growing I don’t know where is the problem.

[@KaiusKing]

Good Video! Thank you.

[@flutteringberret7327]

Thanks for the video. how did you get the dilution factor 4 after mixing 10 ul of cells in 10 ul of trypan blue during counting? I though it should be 2, am I wrong?

[@jianfengwangls]

+fefe adding 10 uL cells into 10 uL of trypan blue makes a dilution factor of 2. Then repeat it to the second trypan blue drop makes another dilution factor of 2.
2X2 = 4

[@angie565656]

Thank you it’s very well done, good quality

[@wenhuili7712]

Thank you

[@dhruvlavania5794]

Can fixed-angle rotors be used to centrifuge HEK293 cells instead of swing-out rotors?

[@kosheeka]

During centrifugation, the centrifugal force (RCF) acts on particles in suspension, causing them to sediment based on their size and density. However, rotor design plays a crucial role in how this force is applied to the cells and impacts their behavior.
Fixed-Angle Rotors vs Swing-out Rotors
• Uneven Sedimentation: In fixed-angle rotors, the sample tubes are held at a fixed angle relative to the rotational axis. This creates a sedimentation gradient within the tube. Cells closer to the bottom experience a higher RCF compared to those near the top of the tube. In the case of swing-out rotors, the sample tubes swing outwards. It allows for a more uniform distribution of the RCF throughout the sample volume.
• Shear Stress: The uneven distribution of RCF can generate shear stress on the cells, particularly those sedimenting at the bottom. This shear stress can damage fragile cells like HEK293, impacting cell viability and integrity. Whereas, swing-out rotors minimize shear stress on the cells. This gentler centrifugation helps preserve the viability and integrity of fragile cells like HEK293, making them ideal for downstream applications.

[@naslunnaslun884]

「ビデオコンテンツはとても素晴らしいです、おめで

[@TheThesun19]

Thank you ;)

[@RanjeetSingh-yo1jb]

Our company manufactures measuring cylinder of hexagonal base.

[@shri8429]

During the subculture u don't take cell count so how we clear take how much cell in ml and how much take growth medium for subculturing.

[@ivyling6347]

Normally you follow manufacturing instructions about the ratio for subculturing. They will say 1:4 to 1:6 or something like that. Therefore, you won't need to count the cells.

[@thunderthunder9251]

How do you determine the appropriate volume of freezing media?

[@kosheeka]

The appropriate volume of freezing media is determined by considering two key factors: the desired final cell concentration post-thaw and the potential for cell loss during the freezing process. Typically, we aim to fill cryogenic vials to 70-80% capacity with freezing media containing a slight volume excess to account for this loss. This approach ensures sufficient viable cells are available for successful experimentation after thawing.

[@misbrby]

SONG PLS?

[@KaiusKing]

What song/Instrumental is this?

[@princyslifestyle2046]

Does suspension cellline gets cloudy after 1 day

[@o.g.5200]

No, cloudiness is usually an indicator of contamination.

[@patricklungu756]

Thank you for an educative video. Do have any video that shows cell stimulation of whole blood?

[@VikramCam]

Yes i have some information regarding this.