Cell Suspension Culture // Plant Biotechnology // Plant tissue culture // Cell culture
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- Опубликовано: 15 апр 2021
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--Embryo culture: • Plant EMBRYO Culture//...
--Callus induction: • Callus induction proce...
--Callus induction from seed: • Callus induction from ...
--The stages of in-vitro seed germination: • Stages of In-vitro see...
--To learn the process of in-vitro seed germination watch this video: • Invitro seed germinati...
-- To learn basics of sterilization techniques watch this video: • Types of Sterilization...
-- To learn all about the Tissue culture lab, the instruments used watch this video: • Plant Tissue culture L...
-- Learn how to fumigate the lab by watching this video : • Fumigation of Lab
-- Visual tour of microbiology lab: • Microbiology lab and t...
-- Visual tour of molecular biology lab: • Molecular biology lab
-- Visual tour of a general life science laboratory: • Life science research ...
This is very helpful and true informative channel
Thanku so much sir☺ very helpful video tomorrow is my practical exam u explained very clearly it really helpful for me thanks alot😊
Tha k you sir it helped me to visualise the whole process I read in my class 12 biotechnology book 😅
Thank you so much sir and also please do cell viablity test and cell density test and also suspension cell extraction . i am searching so many videos but did not found , please make a video , will be thank full for so many people.
Thankuu so much sir the way you explained was very clear and your efforts are very grateful and make more videos like this and make our subject easy ☺️
Thank you so much to making a video on Cell suspension culture. I have searched a lots but you are 1st one who uploaded a video on this topic. Thank you once again for your efforts.
Thank you means a lot
Great 😊
Tnx a lot sir
What is better to make cell suspension from callus or from leaves directly???
Excellent explanation
Thank you :)
Sir during grinding, doesn't cell rupture?
Sir
You are doing a great job. All your videos helped me a lot for my research work.
A humble request... Please do a video on somatic embryogenesis 🙏
It would help us a lot
Ok
Handsome teacher.....❤️👍
Haha thanks for the compliment :)
@@Dr.Rakesh.B 😘😘❤️❤️😘😘😉
Your are super
Thank you :)
Plz provide many more plant biotechnology related project lab videos
Are you also working on guava tissue culture..?
No, Mucuna pruriens
Great video sir very helpful and very concisely made.
Sir, if possible can you also show some techniques related to cell growth measurement parameters of cell suspension cultures.
Ok will do 👍
I have seen so many videos. But he demonstrated excellent with practical demonstration. ❤️👍 I'm hvg agar agar for nutrient medium. Is that fine for liquid and solid medium . And also I'm hvg homogenizer which I use for nano technology nano liposomes. So can I use that homogenizer.
Thank you.
Yeah u can use agar agar. But for liquid medium we don't use any agar. Only for solid we use. And yeah u can use that homogenizer after sterilizing
Sir hw to prepare ms media
Sir , where do you study
Hello sir,
In case of callus culture we do suspension culture... But sir, what should we use for subculturing the plant , when we are dealing with shoot initiation invitro ...
As I have tried shoot multiplication experiment in test tube, now shoot buds are coming out but there is no space for them to grow, so I'll have to shift it from tube to flask,
So please help me with the suitable procedure or suggestions for that ...
Thank you 🙏🙏🙏
Yes, multiple shoots will come. Cut them make sure each Cut has 1 shoot and transfer to new phyta jars with same shoot multiplication media
@@Dr.Rakesh.B okay...thank you so much sir for your speedy reply... , But when should we apply root initiation hormones to the culture ...
Sorry sir, but one more question is there,
Do we need to tightly seal the cap Of the cultures or air is needed inside the Culture tubes ?
Hopefully you will answer whenever you get time to...
Thank you so much once again
🙏🙏
Yes, u need to tight the caps. We also seal them using parafilm or klin wrap. It avoids contamination. And plants do not require air from outside even if you seal it tightly the air inside is enough. Plants utilizes them and creates a cycle.
If you feel u have sub cultured enough of shoots then u can add Rooting hormones and once roots are grown u can take it and go for hardening. It depends on the work and ur objectives.
@@Dr.Rakesh.B thank you soo muchh sir for your kind help 🙏🙏🙏
Sir which chemical I used in tissue culture to prevent contamination ? I don't have PPM then what thing I use
@@Dr.Rakesh.B sir i watched it but what to do if I have contamination after using all sterilization techniques.. Can you suggest me any chemical to mix with media like PPM " Plant Preservation Mixture " Thank you
@@Dr.Rakesh.B really really thank you sir.. I definitely take your advaice, and ya I am little bit doing thing fast and you say about the right thing... Good things take time... Thank you sir love your videos ❤❤
What will be temperature for suspension culture as the callus already in cold
Same temperature to be maintained as callus culture
@@Dr.Rakesh.Bthank you
@@Dr.Rakesh.B sir when can we add elicitors to the suspension cultures... Also is it required subculturing along with same elicitors
@ahlamabdulaziz6513 yes same elicitors and same conditions
Ii this C.roseus callus ?
No Mucuna pruriens
Kaha sa hai sir ap
Karnataka
video is not visible what you are doing.