Thank you so much for sharing this. Please I need clarification of the procedures for sterilization of the filtration kits. You autoclave and what is the other option you mentioned. It's not clear please
When sterilized media is prepared, Pour it at 40 ℃ to 45 ℃ under LAF, After solidify store plates as Inverted position. Or after test also incubate plate as inverted position. Because, 1) The inverted position does not allow the collection of condensed water droplets that could disturb the culture. 2) Inversion reduced the risk of contamination from the air. 3) The nutrients present in the media are easily distributed and available to cells in an inverted position due to gravitational force. 4) Petri dishes are easy to handle when kept in an inverted position as they are generally labeled at the bottom.
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Thank you picked out lot of things
WOW..this is realy helpful thank you!
Really helpful
Thank you so much for sharing this. Please I need clarification of the procedures for sterilization of the filtration kits. You autoclave and what is the other option you mentioned. It's not clear please
1 ) Autoclave Filtration cup & Manifold (121℃ - 15 lbs,
Or
2) Use Ready to use filtration unit
Hi. Great Video. Where can i buy this membrane filter and pump?
Can you please Tell me clearly How to Keep the plates in correct position in incubator ?..I can't get this from your video
When sterilized media is prepared,
Pour it at 40 ℃ to 45 ℃ under LAF,
After solidify store plates as Inverted position.
Or after test also incubate plate as inverted position.
Because,
1) The inverted position does not allow the collection of condensed water droplets that could disturb the culture.
2) Inversion reduced the risk of contamination from the air.
3) The nutrients present in the media are easily distributed and available to cells in an inverted position due to gravitational force.
4) Petri dishes are easy to handle when kept in an inverted position as they are generally labeled at the bottom.
@@p.jchaudhary817 Thank you very much ..
Hi Joshua, what is in your sterile diluent...is it just deionised water?
Can we sterilize the membrane filters,if they are not available in sealed packets?
👍✨
Thanks
@@joshuassozi4982 where are you working?