Microbiology: The spread plate technique

Learn how to make a glass spreader and aseptically spread an aliquot of bacteria evenly onto the surface of an agar plate.

[@MrWhangdoodles]

Excellent video.

[@Mayanfull]

Thanks!! I needed a reminder and this was very useful!

[@farahali463]

u r so good, thank u bc of u I'll take a very good score in the exam

[@MrMRbarati]

Thanks a lot David. I am planning to do this experiment in our lab for the first time. Your demonstration made me feel confident. Cheers

[@zarinhaidernodi9622]

It was e.coli????

[@sawairagul251]

Well explained 👌🏼

[@user-wk2gp3tu8h]

Thank you 💯💯

[@alisaleh1987]

thank you s🇮🇶Thank you, sir. I am from Iraq, and this lesson we have is in biology Thank you very much

[@byronmunashe3827]

Well explain.👍👍👍👍👍

[@vet.doctoraliakbarrehmat2914]

Thanks

[@arunkalaivanan8075]

sir, i have doubt, about why there is moisture alone with the bacterial colony in the end pic?

[@haroldosoares6531]

muito bom, perfeito 👍

[@lauryngrcic6237]

What about the very small amount of inoculum that is inevitably left on the spreader tool? Doesn't that loss of volume affect your CFU calculation if the true volume isn't delivered to the plate? Why doesn't the loss of volume matter?

[@davecummings6328]

Hi Lauryn. You could try to calculate how much is lost based on surface area of the spreader and surface tension of the broth. But my guess is that it's a few uL and the change in colony count is probably well within the error of the technique anyway. All that is to say that I don't think it would impact your results significantly.

[@vetberrypk]

What should be the temperature inside incubator ?

[@josephgaleno5098]

Little late but typically E.coli on petridish can grow at 35c. At this temperature, you should be able to see growth within 24 hours.

[@davecummings6328]

usually 37C, but that depends on the species of bacteria.

[@sushamapawar5842]

I also watch your vidios pleas give a way after dmlt diploma in medical lab tech. Need your guidance sir

[@sushamapawar5842]

I also watch your vidios pleas give a way after dmlt diploma in medical lab tech. Need your guidance sir

[@sarekaya4815]

I tried this method, but the bacteria did not grow as colonies, they grew in the whole plate and I could not count the colonies. What is the reason? thanks

[@fukyoutubestupidfuckinghandles]

This method covered the whole plate so that's how it grew. This method isn't for counting colonies.

[@fukyoutubestupidfuckinghandles]

streak plate method might be better but ive just started so there's probably a better method i dont know

[@ashlykraphy9933]

Sare kaya, You probably need to dilute the sample more so that you get it in countable range which is 20-200 for spread plating technique.

[@pabasarasamarawickrama8404]

Oo😂❤❤o❤oo❤❤❤o❤o❤ oki iiooi❤❤❤k😊kk