Serial Dilutions | Microbiology

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🎥 In today's video, we're exploring the purpose and procedure of serial dilutions used in Microbiology. I hope you will not have any questions on serial dilutions after watching this video.
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[@Osinachi_Silas]

Thank you so much for this explanation... please I further need help, I want to apply bacteria population ranging from 10^6 to 10^8.. I did the serial dilution and plating, after counting the petri dish with 10^-7 gave me a cfu of 30 and because I used 0.1ml for the plating, I ended up having 30,000000. Please how do I get a bacteria population range of 10^6 to 10^8. Thank you

[@MicrobiologyMantra]

The population that you got which is 30,000000 falls between 10^6 and 10^8.

[@Shreyaaa230]

Thankyou so much sir for your explanation....

[@MicrobiologyMantra]

👍🙏☺️

[@hareemkashif5933]

Never seen such a good explanation about this topic ..whole concept has been cleared through this only video...great work.. hat's off to you 🤗

[@MicrobiologyMantra]

Thank you 🙏

[@vaishaly3063]

great and simple explanation ….hats off to you.

[@MicrobiologyMantra]

Thank you 😊

[@mariaqadar6470]

Such a great explanation..

[@MicrobiologyMantra]

Thank you

[@Jawaab01]

Thank you so much for this video.
Such explicit a video.
But I think you made a mistake in writing the formula for calculation of CFUs.

[@brianmarv]

If I were to use vial 3 and 4, how will I standardised / average them? From the reading, they are 2,600,000 CFU to 3,500,000 CFU respectively. Will the average be 3,050,000 CFU?

[@MicrobiologyMantra]

Choose the one with countable CFU.

[@brianmarv]

@@MicrobiologyMantra thank you. Yes, both have countable CFUs, as per your illustration. Though the computed ranges are wide, that's why I am seeking guidance.

[@MicrobiologyMantra]

You cannot take average of two different dilutions. You must take the average of two plates from the same dilution.

[@brianmarv]

Thank you. It now makes sense.

[@fullsunsquirtlestar767]

Can you give us some of your reference, like ebook or journal, i need it for my practical task

[@MicrobiologyMantra]

On what topic?

[@fullsunsquirtlestar767]

@@MicrobiologyMantra about this topic, bacterial dilution

[@fullsunsquirtlestar767]

@@MicrobiologyMantra like, where are you got this bacterial dilution technical and procedure

[@MicrobiologyMantra]

I don’t have any particular reference for this topic. I made this out of my own practice knowledge and resources from the Internet as well. There is lot of information available on this topic on the Internet. I suggest you to summarise and make notes. That will make you more familiar.

[@rishannascaria5831]

Thank you sir 😊 for this simple explanation

[@MicrobiologyMantra]

👍😊

[@derinkamal7284]

Thank you so much for your Video, it was so useful 👏👏👏

[@MicrobiologyMantra]

Good to hear this.👍

[@sarahmaqsood686]

I got everything, but I have one question. When we take 1ml from each tube and transfer it to the next, the concentration of all of them decreases to one 1ml, but the last tube will have 1 ml more bcz it wont be tranfered to the next tube. so how do we bring its volume equal to the rest of the tubes?

[@MicrobiologyMantra]

Concentration does not depend on volume. Example: Prepare a 1000 mL solution of NaCl with 1mg/mL concentration. If you transfer 500 mL to another container, its concentration will still be 1mg/mL only.
Hope you understand.

[@aamodrisbud4204]

Great video i enhanced my knowledge via this :) thanks.

[@MicrobiologyMantra]

Glad it was helpful! Thank you for watching.

[@Shortscience_I]

thank u so much

[@MicrobiologyMantra]

👍

[@alaanaji2833]

Thank you very much for this video. I understood well. I really like how there's no music to allow me to focus. Thanks again😊

[@MicrobiologyMantra]

Glad it was helpful!

[@simranshah3478]

Thank you 😊
Too accurate explanation.!

[@MicrobiologyMantra]

Thank you

[@justsomeone2001]

Thank you so much

[@MicrobiologyMantra]

👍🙏

[@OmmPrakashkar-su3le]

Thank you 👍🏿

[@MicrobiologyMantra]

👍

[@julieann4991]

Amazing explanation! thank you very much for this..

[@MicrobiologyMantra]

Thank you ☺️

[@ifeomaezeanolue997]

This is very very explanatory. Thank you

[@MicrobiologyMantra]

👍 Thank you for watching.

[@nishajalal3735]

Thanku ❤❤❤

[@MicrobiologyMantra]

👍

[@DashrathPogake]

Best explained video..😊

[@MicrobiologyMantra]

Thank you ☺️

[@chumunga648]

It's kind of odd that the narration just disappears for the back half of the video

[@JANANIV-dw9hu]

No one can not explain like you thank you sir

[@MicrobiologyMantra]

Thank you 🙏

[@harshakunjam5754]

Now, I'm able to understand

[@MicrobiologyMantra]

👍

[@khanxada3209]

Great video 👍📸

[@MicrobiologyMantra]

Thank you

[@preciousgarirai8406]

Well explained 👏

[@MicrobiologyMantra]

Thank you ☺️

[@anshumansahoo9057]

Thank you ❤

[@MicrobiologyMantra]

👍

[@SAKSHISHARMA.39]

Thanku so much❤

[@MicrobiologyMantra]

👍