Purine Biosynthesis (De Novo) || Biochemistry || Molecular Biology

This is the video on De novo synthesis of purine nucleotides with its regulation along with a note on analogues used as purine synthesis inhibitors. There are MCQs at the end of video.
Purines and pyrimidines are dietary nonessential compo­nents.
Dietary nucleic acids and nucleotides do not provide essential constituents for the biosynthesis of endogenous nucleic acids
The two purine nucleo­tides of nucleic acids are:
1. Adenosine monophosphate, AMP
2. Guanosine monophosphate, GMP
Purine nucleotides can be synthesized by two pathways:
1. De novo pathway
2. Salvage pathway
References -
1. Pankaja Naik, Biochemistry, 5th edition
2.Rebecca James, Self Assesment and Review of Biochemistry

[@laylajameel1836]

Amazing explanation, thank you

[@Biochemistrybasics]

😊🙏. Please spare a few minutes and do spread the word about the channel among your batchmates and friends

[@rojaa.s5394]

Sir, is the ribonucleotide and ribosyl-5-P are same ? For example GAR is named as glycinamide ribonucleotide or glycinamide ribosyl-5-P.

[@SpizyPopstar]

Nice lecture ..it really helped me to understand

[@Biochemistrybasics]

Glad to know that

[@rojaa.s5394]

5.53 Sir in 5th step you said that FGAR convert to FGAM. My question is, only the term amide Changes to amidine, then why R (FGAR) changes to M (FGAM) sir ?

[@chinmayeechavan5724]

Very nice explanation sir ! Please keep making more videos !

[@Biochemistrybasics]

🙌😊

[@allwibes9126]

Excellent video sir👍🔥
Thank u 😊

[@Biochemistrybasics]

Thanks Jeet 😊

[@RafiRafi-tg1xj]

Sir plz do video on salvage pathway also

[@Biochemistrybasics]

Hey vinay it's already there along with its applied aspect in molecular biology playlist.
Sharing the link too
ruclips.net/video/l9jnlyb3JH4/видео.html

[@fariyaislamsinthi3358]

Thank you sir .It was very well explained.

[@Biochemistrybasics]

Most welcome dear

[@Brighterworld5827]

Sir I found a question in csir net life science from this topic that which no. Carbon or nitrogen would be contributed by which amino acid ?? Should we prepare whole pathway of purine synthesis it seems too long sir please reply

[@Biochemistrybasics]

No need to remember whole pathway. Remember the rate limiting enzymes and regulation of the pathway for entrance exam

[@jamtootSHIN]

Hi, may i know if you have a pdf copy of your references? Looks like very informative thank you.

[@Biochemistrybasics]

Thanks Jamella.

[@pratibhanakum2992]

Very well explained 💯

[@Biochemistrybasics]

🙏😊

[@radhakrishnanravichandran534]

Awesome explanation sir..tq sir

[@Biochemistrybasics]

Thanks dear 🙏😊

[@Soumya-gl4db]

Sir, how synthetic nucleotides are used as drugs?

[@Biochemistrybasics]

They are the structure analogues so used as drugs for the treatment of various diseases

[@Soumya-gl4db]

@@Biochemistrybasics Thank you sir

[@tejatweety9513]

Sir make a video on pyrimidine de novo synthesis

[@Biochemistrybasics]

Will keep it in mind.

[@anjali4883]

Sir why it is called de Novo pathway please reply sir 🙏🙏🙏🙏

[@Biochemistrybasics]

Because there is synthesis of new purines

[@anjali4883]

@@Biochemistrybasics thank you sir...I have one more doubt....which is the regulatory step of IMP pathway and why??🙏

[@drnaazneenmohammed1094]

2 : 40

[@DrMorphineAIIMS]

Awesome sir😊🤗

[@Biochemistrybasics]

Thanks 😊

[@DrMorphineAIIMS]

@@Biochemistrybasics Sir,is it necessary to remember all these step of de Novo whatever u show in it for proff exm????

[@Biochemistrybasics]

@@DrMorphineAIIMS Yes if it's asked as full length questions plus to solve mcqs

[@DrMorphineAIIMS]

@@Biochemistrybasics ok thanku sir

[@ronakgadara6063]

👌👌👌👌

[@Biochemistrybasics]

Thanks Ronak. Stay home and stay safe 😊

[@foodie5466]

Biosynthesis of Purine in plants and animals are same ??

[@Biochemistrybasics]

Almost same

[@foodie5466]

@@Biochemistrybasics it means i can write this in purine biosynthesis of plants ?
In plants also have two pathways for purine biosynthesis that is de novo pathway and salvage pathway??

[@Biochemistrybasics]

@@foodie5466 Yes. You can and yes they have also two pathway one is de novo and other is salvage

[@foodie5466]

@@Biochemistrybasics Okay thanku so much sir

[@swapnayadav4376]

Where it occurs

[@Biochemistrybasics]

Swapna, It occurs in cytosol.

[@swapnayadav4376]

Brain or liver or kidney or all of the the above

[@Biochemistrybasics]

Mainly in the liver and in brain very minor extent.

[@chinmayeechavan5724]

@@swapnayadav4376 this process occurs mainly in cytosol of liver , and is absent in RBC's , WBC's and Brain , in these organ nucleotide are formed via Salvage Pathway