How to measure the pH of culture media - Microbiology Lab Tutorial

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  • Опубликовано: 9 июл 2024
  • In this video tutorial, we will demonstrate the process of using a pH meter to measure the pH of culture media.
    When culturing microbes, it is critical that the media used meets the growth requirements of the target organism. For this reason, it is necessary to measure the pH of culture media during preparation to ensure that the media will perform as intended.
    Most microbes grow best at a neutral or slightly alkaline pH. For this reason, it's no coincidence that the common general growth medium Tryptic Soy Agar is prepared with a pH specification of 7.1 to 7.5.
    00:00 Intro
    00:09 Info about the pH scale
    01:01 History of pH
    01:16 Before you get started
    01:40 Calibrating your pH meter
    03:10 Verifying your pH meter readings
    05:01 Reading pH of culture media
    05:39 Closing info
    In addition to culture media, Hardy Diagnostics offers lab supplies like pH meter calibration buffers:
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