plasmid copy number regulation | What is meant by copy number of plasmid? | low copy number plasmid
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- Опубликовано: 28 авг 2024
- This video describes the detailed mechanism of plasmid copy number control.
this video will answer several questions like
1)What is meant by copy number of plasmid?
2)How is copy number of plasmid determined?
3)What is the average copy number of F plasmid in E coli?
4)What is Ori control copy number?
5)What is high copy number plasmid?
6)What is a benefit for a low copy plasmid?
7)How is copy number of plasmid determined?
8) What are the effects of the metabolic burden of high plasmid copy numbers?
Thank you so much. I was struggling to understand this, I watched your video after going through multiple textbooks, videos and notes. This cleared my concept finally. Thank you so much . God bless .
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So gd n so clearly explained ....I can actually listen ur videos n write my exam without referring any books....Lol idk how ppl understand others videos who speak everything other than subject ( annoying I search for gd video bt only his videos pop up) ur videos deserve to get the trending spot 👌
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Nice explanation
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Hi Arpan! want to say a big THANK YOU for making these videos! help me to understand a lot!! I am self-studying bio, your animation makes the concepts very easy to understand! Am giving every video I watched a big thumb.
Question for video "plasmid copy number regulation": what would lead to a higher number of plasmid? If I am understanding right, both with or without ROP1, RNA I and RNA II tend to bind and lessen the number of plasmids, right? So what would lead to a higher number of plasmid? In a situation when RNA I not replicating?
I am glad to hear that it helped you......my channel aims to give a basic concept on biology for people who are doing self-study or don't have access to good schools.
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@@animatedbiologywitharpan of course! will share your video with friends! Thanks a lot for your work :P!
@@animatedbiologywitharpan That's a very nice goal! and what about my question, do I explained my question?
High copy number plasmids have mutation in their Ori which leads to release the brake and they can increase in number. some plasmids have a mutation in RNAi sequence or lack of ROP protein thus preventing the RNAi-RNAii complex formation. hence these plasmids will be in high copy number. First of its kind is developed in University of California (UC) and the plasmids are named pUC. Some plasmids, such as the pUC series and derivatives, have mutations in Ori itself which allow them to reach very high copy numbers within the bacterial cell. Plasmids based on pBR322 and many cosmids are generally maintained at lower copy numbers. Very large plasmids are often maintained at very low copy numbers per cell while small plasmids tend to have higher copy number.after all the insert in the plasmid sometimes influence the copy number but it’s not clear how.
Thanks. This was really helpful.
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Thankyou soo much sir. You were a big help😊
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Why in F-plasmid having 1-2 copies/cell but Col plasmids are having 15-20 copies/cell .
thank you!
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Can you explain how the termination part is occur?? Pls
Thank you sir
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thanks sir.. can you tell your source book or site as reference??
Mostly it is based on primrose
sir the plasmid in category of high copy number also have both region of ori ,rna1 and rop1 but how are they getting high copies why not rna1 and rop1 is binding rna2 in them?
You do mutation in genes for rop and sequences for RNA I and II to get more copies of plasmid
Sir please make plasmid maintenance
Could you please elaborate
How low plasmid can be converted into high copy number
Not possible to do
Is replication regulated in host cells also? Like if u get a dna vaccine will the plasmid self-replicate in your human cells?
Usually dna vaccines wont work in high eukaryote cells, if they even manage to enter tru electroporation or particle bombardement they will likely be cut into pieces and destroyed before expressing the protein. It also needs the nucleus proteins for transcription (no way it can get in there). If it is a bacteria it will regulate itself I think (using its own encoded proteins or both its own and host proteins that work together)
With nucleus protein I mean that all transcription proteins are inside the nucleus and nowhere else
Dada sound quality ta ektu kharap asche sona jache na thik
Should not be the case ......In my device it sounds proper...don’t know
@Arpan Parichha 3:19 e sound kharap
yaa.. background noise is present
Sir .what is the Full form of rop
ROP is also known as repressor of primer
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@@animatedbiologywitharpan sure sir
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My WhatsApp is 9987997041
Mic problems
Sorry for the inconvenience
What sound of thid
I am sorry but didn’t get your point