Blood Smear Preparation, Wright Staining and blood cells demonstration

Bloos Smear Preparation, Wright Staining and blood cells demonstration
Bloos Smear Preparation,
Wright Staining,
blood cells demonstration,
PBS examination,
RBCs on PBS microscopy,
Hematology- Making a Peripheral Blood Smear,
Neutrophils on PBS,
Eosinophil on PBS,
Basophil on PBS,
Lumphocytes on PBS,
Monocyte on PBS,
Platelets on PBS,
Thin blood smear preparation,
Tongue shaped blood smear,
Wright staining procedure demonstration,
Thin Peripheral Blood smear preparation and Staining,
Wright Stain: Introduction, Principle, Preparation, Procedure, Result-Interpretation, Keynotes, Wright’s Stained [e-mail]/wright-st...
Peripheral blood smear under the microcope showing blood cells and no microbes
Leishman’s stain or Wright’s Blood stain or Giemsa’s stain
Blood Smear Preparation and Staining Practical Lab
Universe84a, Microhub, Medlabsolutions9, Medicallabnotes,
Blood Smear Preparation, Wright Staining, and Blood Cells Demonstration
Blood Smear Preparation
Purpose: Blood smears are prepared to observe the morphology of blood cells under a microscope, which is essential for diagnosing various blood disorders and infections.
Materials Needed:
Glass slides
Capillary tubes or pipettes
Blood sample (preferably fresh)
Gloves
Alcohol swabs
Lancet (if using capillary blood)
Cover slips (optional)
Procedure:
Collect Blood Sample:
If using venous blood, collect the sample using standard venipuncture techniques.
If using capillary blood, clean the fingertip or earlobe with an alcohol swab, puncture with a lancet, and collect the blood with a capillary tube.
Prepare the Smear:
Place a small drop of blood near one end of a clean glass slide.
Hold another slide (spreader slide) at a 30-45 degree angle to the first slide.
Touch the drop of blood with the edge of the spreader slide and allow the blood to spread along the edge.
Quickly and smoothly push the spreader slide forward to create a thin, even smear.
Allow the smear to air dry completely.
Wright Staining
Purpose: Wright staining is used to differentiate and identify blood cell types in the smear.
Materials Needed:
Wright stain solution
Buffer solution (pH 6.4-6.8)
Coplin jars or staining rack
Distilled water
Gloves
Procedure:
Fix the Smear:
Methanol fixation is often included in the Wright stain solution, or the slide can be pre-fixed with methanol for 1-2 minutes.
Staining:
Place the air-dried smear on a staining rack or in a Coplin jar.
Cover the smear with Wright stain solution for 1-3 minutes.
Add an equal amount of buffer solution to the stain on the slide. Mix gently by blowing.
Allow the mixture to stand for 4-6 minutes.
Rinse the slide with distilled water until the smear appears pinkish-red.
Allow the slide to air dry.
Blood Cells Demonstration
Microscopic Examination:
Examine the stained smear under a light microscope using 10x, 40x, and 100x (oil immersion) objectives.
Types of Blood Cells:
Red Blood Cells (RBCs):
Appearance: Biconcave, disk-shaped cells without a nucleus.
Color: Pinkish-red due to hemoglobin staining.
Function: Transport oxygen and carbon dioxide.
White Blood Cells (WBCs):
Granulocytes:
Neutrophils: Multi-lobed nucleus, pale pink to light purple granules.
Eosinophils: Bi-lobed nucleus, bright red-orange granules.
Basophils: Bi-lobed or S-shaped nucleus, dark blue-purple granules.
Agranulocytes:
Lymphocytes: Large, round nucleus with a thin rim of cytoplasm.
Monocytes: Large cell with a kidney-shaped nucleus and abundant cytoplasm.
Platelets (Thrombocytes):
Appearance: Small, irregularly shaped cell fragments.
Color: Light blue cytoplasm with red-purple granules.
Function: Involved in blood clotting.
Clinical Relevance:
RBC Morphology: Anisocytosis (size variation), poikilocytosis (shape variation), and color changes can indicate anemia or other blood disorders.
WBC Differential: The proportion and appearance of different WBC types help diagnose infections, inflammation, and hematologic diseases.
Platelet Count and Morphology: Abnormalities can indicate clotting disorders.
Conclusion
Proper blood smear preparation, Wright staining, and microscopic examination are crucial techniques in hematology. These methods enable the identification and evaluation of various blood cell types, providing essential information for diagnosing and monitoring blood-related diseases and conditions.