Question? At 6 minutes you state Rapid Stat Stain requires no buffer and can skip to the rinse step but then at 7:50 it suggests dipping the slide in buffer solution (?) This video over all is fantastic for explaining the smear staining process but I want to use Stat Stain with less fuss about preparing slide as I'd be doing this at home at the kitchen table with a microscope looking for changes in Eosinophil counts. Could you please clarify for me the difference between 6 minutes and at 7mins 30 seconds? Thanks!
Hi @josephmaloney-jn1bg we appreciate your question and positive feedback. At Hardy Diagnostics we strive to provide a Culture of Service. To help answer your question I have included a section from our instructions for use (IFU) for the Stat Stain aka One Step Wrights Stain below. I believe this will address your needs. For the full IFU for One Step Wrights Stain (Cat. No. SS016) visit the product page on our website and look under the supporting documents tab. hardydiagnostics.com/ss016 Here are the instructions for the One Step Wrights Stain: STAINING PROCEDURE FOR BLOOD SMEARS AND BONE MARROW: 1. Prepare a film of blood or bone marrow on a microscope slide and allow to air dry. 2. Prepare three containers (e.g. coplin jars, or staining dishes). Fill one container with One Step Wrights Stain and the second and third containers with distilled or deionized water. 3. When stain volume in container 1 becomes insufficient, replace the stain. Do not replenish by adding new stain to the old. 4. To prevent evaporation, keep stain tightly covered when not in use. 5. Change the water in container 2 or 3 when an iridescent scum forms on the surface or when a dark blue discoloration occurs. It is very important to keep the rinse water clean. 6. Dip air dried slides in One Step Wrights Stain 15-30 seconds. 7. Dip slide in distilled or deionized water in container 2 for 15-45 seconds. 8. Dip slide in distilled or deionized water in container 3 for 25 seconds, using quick dips. As an alternative, the slide may be "swished" in distilled or deionized water for 25 seconds to remove the stain. 9. Wipe the back of slide. 10. Dry slides in vertical position, on absorbent surface. Do not blot the smear. 11. Apply oil and examine microscopically. * For bone marrow smears, double all the above times. Additionally, if you would like to learn more about the Wright-Giemsa Stain we have prepared a blog that can assists you. hardydiagnostics.com/blog/how-to-do-a-wright-giemsa-stain
Hi @josephmaloney-jn1bg we appreciate your question and positive feedback. At Hardy Diagnostics we strive to provide a Culture of Service. To help answer your question I have included a section from our instructions for use (IFU) for the Stat Stain aka One Step Wrights Stain below. I believe this will address your needs. For the full IFU for One Step Wrights Stain (Cat. No. SS016) visit the product page on our website and look under the supporting documents tab. hardydiagnostics.com/ss016 Here are the instructions for the One Step Wrights Stain: STAINING PROCEDURE FOR BLOOD SMEARS AND BONE MARROW: 1. Prepare a film of blood or bone marrow on a microscope slide and allow to air dry. 2. Prepare three containers (e.g. coplin jars, or staining dishes). Fill one container with One Step Wrights Stain and the second and third containers with distilled or deionized water.
3. When stain volume in container 1 becomes insufficient, replace the stain. Do not replenish by adding new stain to the old. 4. To prevent evaporation, keep stain tightly covered when not in use. 5. Change the water in container 2 or 3 when an iridescent scum forms on the surface or when a dark blue discoloration occurs. It is very important to keep the rinse water clean. 6. Dip air dried slides in One Step Wrights Stain 15-30 seconds. 7. Dip slide in distilled or deionized water in container 2 for 15-45 seconds. 8. Dip slide in distilled or deionized water in container 3 for 25 seconds, using quick dips. As an alternative, the slide may be "swished" in distilled or deionized water for 25 seconds to remove the stain. 9. Wipe the back of slide. 10. Dry slides in vertical position, on absorbent surface. Do not blot the smear. 11. Apply oil and examine microscopically. * For bone marrow smears, double all the above times.
Additionally, if you would like to learn more about the Wright-Giemsa Stain we have prepared a blog that can assists you. hardydiagnostics.com/blog/how-to-do-a-wright-giemsa-stain
Could I ask if Phosphate-buffered saline is also a possible buffer for the wright-giemsa stain or is it preferred to use the hematology rinse solution? I'm conducting a research paper for a school requirement
Really good video. Needs more traction and views. Underrated
Glad you liked it!
Question? At 6 minutes you state Rapid Stat Stain requires no buffer and can skip to the rinse step but then at 7:50 it suggests dipping the slide in buffer solution (?) This video over all is fantastic for explaining the smear staining process but I want to use Stat Stain with less fuss about preparing slide as I'd be doing this at home at the kitchen table with a microscope looking for changes in Eosinophil counts. Could you please clarify for me the difference between 6 minutes and at 7mins 30 seconds? Thanks!
Hi @josephmaloney-jn1bg we appreciate your question and positive feedback. At Hardy Diagnostics we strive to provide a Culture of Service. To help answer your question I have included a section from our instructions for use (IFU) for the Stat Stain aka One Step Wrights Stain below. I believe this will address your needs. For the full IFU for One Step Wrights Stain (Cat. No. SS016) visit the product page on our website and look under the supporting documents tab. hardydiagnostics.com/ss016
Here are the instructions for the One Step Wrights Stain:
STAINING PROCEDURE FOR BLOOD SMEARS AND BONE MARROW:
1. Prepare a film of blood or bone marrow on a microscope slide and allow to air dry.
2. Prepare three containers (e.g. coplin jars, or staining dishes). Fill one container with One Step Wrights Stain and the second and third containers with distilled or deionized water.
3. When stain volume in container 1 becomes insufficient, replace the stain. Do not replenish by adding new stain to the old.
4. To prevent evaporation, keep stain tightly covered when not in use.
5. Change the water in container 2 or 3 when an iridescent scum forms on the surface or when a dark blue discoloration occurs. It is very important to keep the rinse water clean.
6. Dip air dried slides in One Step Wrights Stain 15-30 seconds.
7. Dip slide in distilled or deionized water in container 2 for 15-45 seconds.
8. Dip slide in distilled or deionized water in container 3 for 25 seconds, using quick dips. As an alternative, the slide
may be "swished" in distilled or deionized water for 25 seconds to remove the stain.
9. Wipe the back of slide.
10. Dry slides in vertical position, on absorbent surface. Do not blot the smear.
11. Apply oil and examine microscopically.
* For bone marrow smears, double all the above times.
Additionally, if you would like to learn more about the Wright-Giemsa Stain we have prepared a blog that can assists you. hardydiagnostics.com/blog/how-to-do-a-wright-giemsa-stain
Hi @josephmaloney-jn1bg we appreciate your question and positive feedback. At Hardy Diagnostics we strive to provide a Culture of Service. To help answer your question I have included a section from our instructions for use (IFU) for the Stat Stain aka One Step Wrights Stain below. I believe this will address your needs. For the full IFU for One Step Wrights Stain (Cat. No. SS016) visit the product page on our website and look under the supporting documents tab. hardydiagnostics.com/ss016
Here are the instructions for the One Step Wrights Stain:
STAINING PROCEDURE FOR BLOOD SMEARS AND BONE MARROW:
1. Prepare a film of blood or bone marrow on a microscope slide and allow to air dry.
2. Prepare three containers (e.g. coplin jars, or staining dishes). Fill one container with One Step Wrights Stain and the second and third containers with distilled or deionized water.
3. When stain volume in container 1 becomes insufficient, replace the stain. Do not replenish by adding new stain to the old.
4. To prevent evaporation, keep stain tightly covered when not in use.
5. Change the water in container 2 or 3 when an iridescent scum forms on the surface or when a dark blue discoloration occurs. It is very important to keep the rinse water clean.
6. Dip air dried slides in One Step Wrights Stain 15-30 seconds.
7. Dip slide in distilled or deionized water in container 2 for 15-45 seconds.
8. Dip slide in distilled or deionized water in container 3 for 25 seconds, using quick dips. As an alternative, the slide
may be "swished" in distilled or deionized water for 25 seconds to remove the stain.
9. Wipe the back of slide.
10. Dry slides in vertical position, on absorbent surface. Do not blot the smear.
11. Apply oil and examine microscopically.
* For bone marrow smears, double all the above times.
Additionally, if you would like to learn more about the Wright-Giemsa Stain we have prepared a blog that can assists you. hardydiagnostics.com/blog/how-to-do-a-wright-giemsa-stain
Clear, concise & helpful. Will definitely order supplies thru Hardy Diagnostics. Thank you.
Glad it was helpful! Please do!
The video on the One step stain is confusing, it says you do not need to use buffer, yet you say to put it in the buffer. Very confusing.
Could I ask if Phosphate-buffered saline is also a possible buffer for the wright-giemsa stain or is it preferred to use the hematology rinse solution? I'm conducting a research paper for a school requirement
Tell me the preparation of giemsa liquid before staining
Thank you so much. That was perfect!
Glad it helped!
can you find borrelia with that?
Good luck dear