How to prepare the perfect Gram stain - Gram staining procedure
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- Опубликовано: 11 июн 2024
- Hardy Diagnostics is your complete Microbiology supplier. To learn more about Hardy Diagnostics visit our new website. bit.ly/3Jfoj9j
In this educational video from Hardy Diagnostics, we detail what a Gram stain is, what is the purpose of a Gram stain procedure, and share how you can perform a perfect Gram stain test.
A written step-by-step version of this tutorial can be found on our blog: hardydiagnostics.com/blog/gra...
Q-Slide Gram (Cat. no. Z302)
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Gram Stain Kit Advanced™ (Cat. no. GK400A)
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00:00 History of the Gram stain
00:20 The importance of the Gram stain
00:38 Types of bacteria
00:53 The cell wall
01:27 Beginning the Gram stain
02:09 Fixing the slide with methanol
02:53 Crystal violet
03:02 Deionized water
03:12 Gram's iodine
03:26 Rinse with deionized water
03:37 Decolorizer
04:58 Color of Gram positive vs Gram negative
05:11 Counterstain with safranin
05:26 Rinse and dry
05:50 Summary
06:13 How to view slide under a microscope
06:30 Results
06:47 Ordering info and close
Check out our other laboratory tutorials on our blog:
hardydiagnostics.com/blog/lab...
Filmed and Edited by Brian Tom
#Microbiology #GramStain #Bacteria #HardyDiagnostics #gramstaining 
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I felt like I was in a university lecture. brilliant presentation. thank you for explaining it in such detail.
You are welcome. Glad the video was useful and detailed!
I hope everyone enjoys our new Gram Stain tutorial!
We put this video together because we saw a great need to train Techs on the proper way to get accurate and consistent results when it comes to performing the all-important Gram Stain. Please let us know if you have any comments...we would love to hear from you!
Jay Hardy
no
Really helpful for my dermatology Toacs station.Thanks alog.Beautifully explained
YES FOR SURE. IT WAS WORTH WATCHING.
yh
Very nicely done and helpful. I do wish the video showed the rinses being done over a staining tray and properly disposed of instead of being washed down a sink!!
Great video, easy to understand for non-microbiologists!
Great to hear!
I'll need to watch this over 3 more times. So much information .
Thanks guys
So happy to hear this! We love to be a part of ongoing learning for all our viewers!
Great video! Wonderful info and beautiful graphics. Perfect video for either those just learning microbiology & gram staining, or for those looking for a refresher.
Awesome! Thank you!
I appreciate this video. It will be helpful for my Micro Lab. Thank you. 💗
Glad it was helpful!
Wonderful explanation! Thank you
Glad it was helpful!
It's very useful for me to do practicals... Thank you so much .... Love from India🇮🇳❤️❤️
Glad it was helpful! Love to see it! Cheers from America!
@@HardyDx but if we pour decoloriser more then g postive Bacteria will lose color right ?? So we have to not pour more decoloriser until all the stain comes out ??
Thanks, very helpful me to understand gram positive and negative bacteria.
Glad it was helpful!
Awesome graphics and brilliant explanation! Caught me off guard with the 'culture of service' pun in the promotion D:
Glad you liked it! Service is part of our company culture here at Hardy Diagnostics.
Well done, well said. Thank you
Thanks for watching!
Very thorough and very accurate, bravo Hardy Diagnostics
Thank you so much sir it is really sooooo good 🤩👍
You are most welcome.
Thank you for this interesting video ❤
Glad you enjoyed it!
Nice video. 👍🏻
I’ve done a few gram stains in the past and the technique is a little tricky just following a procedure. The video helps to show exactly how it should happen.
It's amazing
gram stain plays vital role in identifying bacterial morphology and the best way to take prudent choice of antibiotics
Good job.
Many thanks!
Thank you.
You're welcome!
Thank you for this video. It is very informative. I hope you can remove the background music or lower the volume down. Thank you
Thank you for taking the time to watch our video and for sharing your feedback with us. We truly appreciate your input, and we want you to know that your suggestions will definitely be considered in our future video planning.
Regarding the background music volume, we understand your concern. Unfortunately, we are unable to make adjustments to previously posted videos without affecting our current viewership and traffic. However, please know that we are continuously striving to improve the quality of our content based on valuable feedback like yours.
Once again, thank you for being a loyal viewer, and we hope you continue to find our videos informative and engaging.
thanks!
Our pleasure!
Can absolute alcohol be used to fix the slide?
Thanks sir
You are very welcome. Glad it was helpful.
makasi bang
I assume the videos that used heat to fix the bacteria are representing the old method
Sir I am Bangladeshi. I have test sputum for gram stain... Pus cells... ( ++).. Gram positive cocci... Plenty..l want to know about this report...
Thanks for practical
How much it costs to buy ur products from Pakistan
Hello Hafiz Umar! Thank you for the comment. Please navigate to our customer form using this link, hardydiagnostics.com/contact-us/?form_id=4. Select "General Questions" from the drop down menu and fill out the form. We will reach out to you as soon as possible. Until then, enjoy the rest of our RUclips videos 👍
Which decolourizer use pananum
Hi Sandy! Our manual gram stain kit (Cat. No. GK400A) uses a fast-method, 75% acetone, 25% alcohol ratio. Hope this helps!
@@HardyDx thanks you 😀
At @1:25 you've showed that peptidoglycan layer is stained itself. It's not true. It's the cytoplasm that takes up the gram stain.
What tune is this
How price if you want to buy
Hi, Try & Go RUclips Channel! Thank you for the inquiry. Please navigate to our customer form using this link, hardydiagnostics.com/contact-us/?form_id=4. Select "General Questions" from the drop down menu and fill out the form. We will reach out to you as soon as possible. Until then, enjoy the rest of our RUclips videos 👍
Atay oy. Ga labad akong ulo
Iam pilla premmmmmm💥
Wst na kod**ka
˚✧₊⁎❝᷀ົ≀ˍ̮ ❝᷀ົ⁎⁺˳✧
not a very good technique by the fellow and the method/procedure used its bad. usually you dont attack directly the sample with stain or water or whatever you pour on it. heat its not bad at all, just not direclty for more than 5 sec, like in flames works good enough... air dry could contaminate your sample and/or could have alive bacteria (wich isnt bad at all either but it depends on what you want, generaly dont want alive bacteria so you dont contaminate more tools in your lab).
Sorry, I couldn't watch all the video because of the music, but otherwise what I did see was informative. That kind of cheesy not-even techno-kinda royalty free noise is just too pervasive for some people to focus on what they actually want to hear.
Quit yapping.