How to prepare the perfect Gram stain - Gram staining procedure

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  • Опубликовано: 21 ноя 2024

Комментарии • 83

  • @6ftS
    @6ftS 2 года назад +59

    I felt like I was in a university lecture. brilliant presentation. thank you for explaining it in such detail.

    • @HardyDx
      @HardyDx  2 года назад +3

      You are welcome. Glad the video was useful and detailed!

  • @juliakorolenko8788
    @juliakorolenko8788 3 года назад +13

    Great video, easy to understand for non-microbiologists!

    • @HardyDx
      @HardyDx  2 года назад

      Great to hear!

  • @raymundo5200
    @raymundo5200 3 года назад +63

    I hope everyone enjoys our new Gram Stain tutorial!
    We put this video together because we saw a great need to train Techs on the proper way to get accurate and consistent results when it comes to performing the all-important Gram Stain. Please let us know if you have any comments...we would love to hear from you!
    Jay Hardy

    • @colinleungcm
      @colinleungcm 3 года назад

      no

    • @user-lh6vr6vs4h
      @user-lh6vr6vs4h 2 года назад

      Really helpful for my dermatology Toacs station.Thanks alog.Beautifully explained

    • @MelomaniacEarth
      @MelomaniacEarth 2 года назад

      YES FOR SURE. IT WAS WORTH WATCHING.

    • @philomenadodoo6228
      @philomenadodoo6228 Год назад

      yh

    • @mostlymicrobes
      @mostlymicrobes 11 месяцев назад

      Very nicely done and helpful. I do wish the video showed the rinses being done over a staining tray and properly disposed of instead of being washed down a sink!!

  • @leannajulien4175
    @leannajulien4175 2 года назад +6

    I'll need to watch this over 3 more times. So much information .
    Thanks guys

    • @HardyDx
      @HardyDx  2 года назад +1

      So happy to hear this! We love to be a part of ongoing learning for all our viewers!

  • @sukishr2349
    @sukishr2349 2 года назад +7

    It's very useful for me to do practicals... Thank you so much .... Love from India🇮🇳❤️❤️

    • @HardyDx
      @HardyDx  2 года назад +1

      Glad it was helpful! Love to see it! Cheers from America!

    • @jennyvarshavski3138
      @jennyvarshavski3138 Год назад

      @@HardyDx but if we pour decoloriser more then g postive Bacteria will lose color right ?? So we have to not pour more decoloriser until all the stain comes out ??

  • @virensingh-sw5hk
    @virensingh-sw5hk 4 месяца назад +2

    Keep it up gentlemen

    • @HardyDx
      @HardyDx  3 месяца назад

      Thanks, will do.

  • @itanruiz7828
    @itanruiz7828 4 месяца назад +2

    Works very great!
    Thank you.

    • @HardyDx
      @HardyDx  4 месяца назад

      You're welcome!

  • @lojohnson4694
    @lojohnson4694 3 года назад +6

    Great video! Wonderful info and beautiful graphics. Perfect video for either those just learning microbiology & gram staining, or for those looking for a refresher.

    • @HardyDx
      @HardyDx  2 года назад +1

      Awesome! Thank you!

  • @Riftshroud
    @Riftshroud Год назад +1

    Awesome graphics and brilliant explanation! Caught me off guard with the 'culture of service' pun in the promotion D:

    • @HardyDx
      @HardyDx  Год назад

      Glad you liked it! Service is part of our company culture here at Hardy Diagnostics.

  • @daudbaig5109
    @daudbaig5109 Год назад

    gram stain plays vital role in identifying bacterial morphology and the best way to take prudent choice of antibiotics

  • @kadhimali266
    @kadhimali266 3 года назад +3

    Thanks, very helpful me to understand gram positive and negative bacteria.

    • @HardyDx
      @HardyDx  2 года назад

      Glad it was helpful!

  • @sparkleberrypop
    @sparkleberrypop 7 месяцев назад

    Thank you for this video. It is very informative. I hope you can remove the background music or lower the volume down. Thank you

    • @HardyDx
      @HardyDx  7 месяцев назад

      Thank you for taking the time to watch our video and for sharing your feedback with us. We truly appreciate your input, and we want you to know that your suggestions will definitely be considered in our future video planning.
      Regarding the background music volume, we understand your concern. Unfortunately, we are unable to make adjustments to previously posted videos without affecting our current viewership and traffic. However, please know that we are continuously striving to improve the quality of our content based on valuable feedback like yours.
      Once again, thank you for being a loyal viewer, and we hope you continue to find our videos informative and engaging.

  • @vanillaaffogato9985
    @vanillaaffogato9985 3 месяца назад +1

    We're always uses bunsen burner to drying on every step. Will it impact the result?

    • @HardyDx
      @HardyDx  3 месяца назад

      @vanillaaffogato9985 We recommend methanol because it typically results in a clearer stain. Over-heating of organism on a slide can sometimes cause it to deform or change shape due to heat exposure. Methanol fixed gram positive organisms are also less likely to de-colorize during the staining process.

    • @vanillaaffogato9985
      @vanillaaffogato9985 24 дня назад

      @@HardyDx wow thank u very much. Very kind of u

  • @BriONeil
    @BriONeil 2 года назад +3

    I appreciate this video. It will be helpful for my Micro Lab. Thank you. 💗

    • @HardyDx
      @HardyDx  2 года назад +1

      Glad it was helpful!

  • @mikevanderman2727
    @mikevanderman2727 2 месяца назад +1

    Love it

  • @christophercatani8820
    @christophercatani8820 3 года назад +6

    Very thorough and very accurate, bravo Hardy Diagnostics

  • @leekspinner
    @leekspinner Год назад +1

    I assume the videos that used heat to fix the bacteria are representing the old method

  • @aliza.hyder_
    @aliza.hyder_ 2 года назад +3

    Wonderful explanation! Thank you

    • @HardyDx
      @HardyDx  2 года назад

      Glad it was helpful!

  • @Blessing.x0
    @Blessing.x0 Год назад +2

    Can absolute alcohol be used to fix the slide?

  • @BAEK611
    @BAEK611 7 дней назад +1

    5:45 good... i wll do like this

  • @DoniaNabil-li2fg
    @DoniaNabil-li2fg 8 месяцев назад +1

    Thank you for this interesting video ❤

    • @HardyDx
      @HardyDx  8 месяцев назад

      Glad you enjoyed it!

  • @hafizumar5732
    @hafizumar5732 Год назад +2

    Thanks for practical
    How much it costs to buy ur products from Pakistan

    • @HardyDx
      @HardyDx  Год назад +1

      Hello Hafiz Umar! Thank you for the comment. Please navigate to our customer form using this link, hardydiagnostics.com/contact-us/?form_id=4. Select "General Questions" from the drop down menu and fill out the form. We will reach out to you as soon as possible. Until then, enjoy the rest of our RUclips videos 👍

  • @13anesthesiamithalipawashe57
    @13anesthesiamithalipawashe57 2 года назад +4

    Thank you so much sir it is really sooooo good 🤩👍

    • @HardyDx
      @HardyDx  2 года назад

      You are most welcome.

  • @DanTheisen
    @DanTheisen 3 года назад +5

    Nice video. 👍🏻

    • @DanTheisen
      @DanTheisen 3 года назад

      I’ve done a few gram stains in the past and the technique is a little tricky just following a procedure. The video helps to show exactly how it should happen.

  • @sergiobotello9046
    @sergiobotello9046 3 года назад +3

    Well done, well said. Thank you

    • @HardyDx
      @HardyDx  2 года назад +1

      Thanks for watching!

  • @kingkura
    @kingkura Год назад +1

    Good job.

  • @Spandan-Adak
    @Spandan-Adak Год назад

    At @1:25 you've showed that peptidoglycan layer is stained itself. It's not true. It's the cytoplasm that takes up the gram stain.

  • @ranyan226
    @ranyan226 2 года назад +1

    Sir I am Bangladeshi. I have test sputum for gram stain... Pus cells... ( ++).. Gram positive cocci... Plenty..l want to know about this report...

  • @Kristenesfiles
    @Kristenesfiles Год назад +1

    thanks!

  • @MunyanzizaKevin
    @MunyanzizaKevin Год назад

    It's amazing

  • @AtayeroValentina
    @AtayeroValentina Год назад

    Thank you.

  • @mahbubajannat9907
    @mahbubajannat9907 Год назад +1

    Thanks sir

    • @HardyDx
      @HardyDx  Год назад

      You are very welcome. Glad it was helpful.

  • @trygoyoutubechannel399
    @trygoyoutubechannel399 Год назад

    How price if you want to buy

    • @HardyDx
      @HardyDx  Год назад

      Hi, Try & Go RUclips Channel! Thank you for the inquiry. Please navigate to our customer form using this link, hardydiagnostics.com/contact-us/?form_id=4. Select "General Questions" from the drop down menu and fill out the form. We will reach out to you as soon as possible. Until then, enjoy the rest of our RUclips videos 👍

  • @sandymaharajan4868
    @sandymaharajan4868 Год назад

    Which decolourizer use pananum

    • @HardyDx
      @HardyDx  Год назад +1

      Hi Sandy! Our manual gram stain kit (Cat. No. GK400A) uses a fast-method, 75% acetone, 25% alcohol ratio. Hope this helps!

    • @sandymaharajan4868
      @sandymaharajan4868 Год назад

      @@HardyDx thanks you 😀

  • @pinkpebble_
    @pinkpebble_ Год назад

    makasi bang

  • @leonardodegregorio7383
    @leonardodegregorio7383 Год назад

    What tune is this

  • @TheModernVictorian
    @TheModernVictorian 7 месяцев назад

    Sorry, I couldn't watch all the video because of the music, but otherwise what I did see was informative. That kind of cheesy not-even techno-kinda royalty free noise is just too pervasive for some people to focus on what they actually want to hear.

  • @hansmenares3850
    @hansmenares3850 Год назад +1

    not a very good technique by the fellow and the method/procedure used its bad. usually you dont attack directly the sample with stain or water or whatever you pour on it. heat its not bad at all, just not direclty for more than 5 sec, like in flames works good enough... air dry could contaminate your sample and/or could have alive bacteria (wich isnt bad at all either but it depends on what you want, generaly dont want alive bacteria so you dont contaminate more tools in your lab).

  • @CorruptEggs
    @CorruptEggs Месяц назад

    How to prep a Gram stain procedure:
    Beginning of vid: the atom was theorized by John Dalto...

  • @premkumarbuddavarapu2641
    @premkumarbuddavarapu2641 2 года назад +1

    ˚✧₊⁎❝᷀ົ≀ˍ̮ ❝᷀ົ⁎⁺˳✧

  • @kranthiel5034
    @kranthiel5034 2 года назад

    Iam pilla premmmmmm💥

  • @diomelgella3010
    @diomelgella3010 Год назад

    Atay oy. Ga labad akong ulo