Detecting an antigen with an ELISA test
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- Опубликовано: 30 июл 2024
- Welcome back into the Human Anatomy and Physiology lab! This video describes the protocol that we carry in this lab at USF to detect the presence of a specific antigen. Although some protocols may do things a bit differently, here we use the protocol and the tools provided in the Bio-Rad ELISA Immuno explorer kit.
Link to the ELISA protocol: 1.cdn.edl.io/mL5I9CK8k6rAEpM4...
I am not a trained health professional and I am not teaching how to perform such protocol outside a classroom through this video.
This video is just a way to show Human A&P students how to perform a basic ELISA test to detect the presence of an antigen
If you have any comments that you think can benefit the video and add valuable information please do not hesitate to share with everyone. But if you have critiques, please keep them for yourself; they are neither useful nor constructive and simply may not apply to the circumstances (I am just demonstrating techniques applicable to an introductory course in Human A&P and I do not pretend to be a certified laboratory technician).
Music:
Find your way by Anno Domini Beats
Grand Avenue by Text Me Records / Bobby Renz
Sunspot by Jeremy Blake
The Shade by Text Me Records / Social Work
Great video man, very helpful. Thanks!
thanks from Marmara University, Turkey.
Thank you!!
I have a question in my mind, why do we use towel for absorbance. isn't just spill the water into the sink sufficient?
Thanks
What is gray zone calculations that is +-10%
Why did the antigen stick to wall in the first step . Is it a special well ?
Why can't we pippette out the liquid? Why to we have to dab it onto tissue paper?
when antibody and enzyme linked antibody binded with antigen which colour should be yield??
😢❤❤😮❤😮❤😅❤
When can we expect these kinds of tests for the COVID19 antigens?
Such assays actually already exist. Here I only demonstrate a simple ELISA protocol aimed at teaching students the principle behind ELISA, but for clinical assays, the methods are often a bit more elaborate. You can learn more about how an ELISA assay can be used to diagnose SARS-CoV 2: www.epitopediagnostics.com/covid-19-elisa
Why do u wash it the first time if there nothing to bind to it?
Wash the excess antigen that didn't stick to the well wall
what type of ELISA is this?
Here I demonstrated how to perform an ELISA assay to test for the presence of antigens. But other protocols can test for the presence/absence of other ligands (i.e. protein) in a liquid sample using antibodies directed against the protein to be measured.