Vitamin-A, Vitamin-E & Vitamin-D Analysis Using HPLC_Part-1 (Sample Preparation)
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- Опубликовано: 28 сен 2024
- Vitamin-A, Vitamin-E and Vitamin-D are most common among the fat-soluble vitamins. Quantitative determination of Vitamin-A and Vitamin-E in food are most important test parameter specially for food samples. Quantification of the Vitamin-A, D & E using HPLC is most precise and widely used method. Sample preparation is very crucial step of this test. This video represents a detailed procedure of the sample preparation for the determination of Vitamin-A, Vitamin-E & Vitamin-D using HPLC.
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Video Link:
Instrumental Analysis of Vitamin A & E: • Vitamin-A & Vitamin-E ...
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Great work guys..the topics are really useful for food tech students... Thanks for sharing vdos..keep it up
Thank you so much. Stay with us.
Great ! Good work guys !!! you're the best. Please if it's possible, could you make a video about the analysis of Aflatoxins, Vitamin C, Sorbic acid.
Thank you. We will upload video for vitamin c, sorbic acid and aflatoxins very soon. Stay with us.
Thank you for sharing knowledge 😉
Welcome. Stay with us.
Excellent 👌 work 👍👍 thanks for sharing... It is very useful... Pls upload how to find calibration curve ..
Noted
Thank you for sharing your knowledge !!!
Is it possible to upload a preparation of vit. D2 and D3 standards for HPLC analysis?
Same sample preparation for D3 & D2
At the end, Vitamin A and Vitamin D will be a solid at room temperature. How do you distinguish the two?
Hello, After the first addition of water, foam is formed, and the layers do not separate, where can the problem be?
Add extra ethanol to remove foam
Hi, Is the same for Vitamin D analysis? I would like to know how the extraction for Vitamin D2 and D3 is done.
Same procedure
@@MicroChemsExperiments ok then thankyou. Is it possible to upload an instrumental session for D2 and D3.
Please make videos for vit b1,b2 and b3 as well
Noted
Please 🙏 make video on water soluble video
Can you explain without condesner can we proceed this analysis will it work or not
Can not.all evaporate.ccondencer or long glass tube required air cooling condencer.
Yes. A condenser is must needed
could you please provide the reference for this procedure
Thank you for sharing your knowledge !!! and You can share a reference that you used in this method for me please
It is a laboratory generated method
How can we extract vitamin A , D and E in cooking Oil
Same method
Thank you so much ma’am. I have a doubt. I am a PhD scholar so I will add reference for this protocol , so which reference should I give?
AOAC method reference
Please guide about Cooking oil
Same method
@@MicroChemsExperimentsOK I try it
Can we use n-heptene instead n-hexane?
Super
Thanks
Please help. We need to analyze vit B3(niacin) can we use this extraction protocol?
We are already working on Vitamin B3. Stay with us.
Thanks so much..💚💚
You are most welcome
Can we take liquid and semi solid sample..... In the same method
Yes
Share gc calibration video plz
L-ascobic acid ?
Yes. It is used as antioxidant during saponification
THANKS
Welcome
Is this process can applicable for liquid sample of Vitamin
Yes
@@MicroChemsExperiments can u please tell me about instrument condition for vitamin D .. please because my m pharmacy project is to find out vitamins content in juice and fortified it
What if sample is in liquid like milk
Take 10 ml milk sample and do accordingly
can we use this method for vitamin D
Yes. This method is also applicable for Vitamin-D as it is also a fat soluble vitamin.
What is the name of this methode ?
Extraction with Saponification
@@MicroChemsExperiments asque vous pouvez détailler svp
How to prepare raw milk sample?
Sample precedure as described in this video.
Part 2
Please wait
What sample taken for analysis
I have a lab experiment to do, and it includes using oranges and determining the vitamin c content in them. Would this procedure work on the liquid juice of the orange? Thank you so much.
Yes, absolutely
can you give us the equation of saponification reaction plz
We will make video later
can we use it for dairy products
Yes. You can
Plz make one vedio on food sample analysis at industrial level.. Kind request🙏🏼🙏🏼
Which test parameter of food do you want?
@@MicroChemsExperiments i want to learn about sensory evaluation of food at industrial levels!
@@StunnedWithMe Taste, Odor are thr sensory evaluation. You can do it by yourself.
Just take smell of the sample or take taste of that sample and write the observations in your reports.
Heyy... Can be use same method for Vitamin D2 analysis
Nice thanks. Can you share the calculation for the percentage claim.
Thank you for uploading
Make some videos using GC GLC machine plz
Which tests you want video for??
Hey could you please specify the official method that you followed?
It is a laboratory developed in-house method
Can you share material of this experiment. Plzz.
We don't provide any document.
What is the sample that you have taken?
Ground biscuit sample
Always in distinction we are in the second part🌹🌹🌹🌹
Will upload soon
thank you so much
You're welcome!
can you give us the equation of saponification reaction plz
i mean the sample + ethanolo + l-ascorbic acid + potassium hydroxide, why are those used and what do they give out as a product?
We will make video later
Very Useful.. Thanks a lot..
Thanks for being with us.
Soap analysis
Which parameter?
Bravo!
Thanks
perfect
What about the standard preparation and calibration curve?
We will upload another video (Part 2) for Vitamin A & E analysis.
Stay with us for mobile phage, standard preparation and instrumental conditions.
@@MicroChemsExperiments I can not wait Thank you for this wonderful work
Please make video for vitamin D analysis Also
@@chemmuhammad8471 We will work for Vitamin D soon. Stay with us.
What is the category food they are determined in?
Where is refference?
What is the limit of each vitamin which this method can determine?
Almost all kinds of food you can analyze with this method
No specific reference method is possible to say. It is in-house developed method. We took idea from AOAC method of retinol determination and other journals.
@@omarsayed2458 LOD 0.1ppm
And LOQ 0.5ppm