is the preparation method for vitamin D standard the same as vitamin A and E and what is the wavelength in which we detect vitamin D and is calculations the same also?
I would recommed HPLC grade solvents, and the use of fluorescence detection (for vit E exition 280 and emission 325nm, vit A ex 342 nm, em 476 nm) to get much better selectivity and sensitivity.
@@MicroChemsExperiments yes I know its from Sigma Aldrich, but I need the product number because when I make a search for the vitamin, it gave me several ones, and I don't know which one should I choose
Thank you very much. And for the filter that you used for the filtration of the methanol, what is the type of the material (PTFE or Cellulose or Nirile or Glass fiber etc..) and the porosity that should I used? If it's possible the product number
@@aymenogkush Cellulose membrane filter. Look up to the video. Details specification is seen when using the filter peper in this video. Product number is not avaulable for this product. You can purchase this from the local market.
Filtering MeOH, sonicate it and then putting inside a piece of cork, bad techniques and sources of contamination are dressed here as an expert advice in just 3 min.
I kept watching the video and now I understand that peak tailing, you're destroying your instrument, mister. Worst of it all is that you must be teaching people both in real life and through your videos, I'm sorry for them.
@@velimattiollilainen Peak tailing is caused by different migration speeds of the analite inside the column, which indicates a poor performance of the system. Areas indeed will be different because because that method isn't suitable for any kind of separation. It can't be a matter of playing the edges on a latter integration when you have no reproducibility to start with.
Thank you so much for this very detailed and informative video. This is a great help. Also would like to ask if you have video for analysis of water soluble vitamins.
@@MicroChemsExperiments the videos you provided are really fantastic. It attracted my attention due to Covid-19. I was share your Videos to my students.
This is very useful . Thank you MicroChem
Thank you... Most awaited video...
You are most welcome. Support us by sharing this channel.
Thank you so much for such informative content. Can you please provide the reference source for this protocol?
No specific reference. This is a in-house developed method
if we want to publish work with this protocol how can we cite the reference for it?
Why stock solution Vitamin A or E is made saponification ? almost standard prepare dilute solvent already to inject.
is the preparation method for vitamin D standard the same as vitamin A and E and what is the wavelength in which we detect vitamin D and is calculations the same also?
Sample preparation is same but instrumental analysis is different. We will upload instrumental condition later. Stay with us.
@@MicroChemsExperiments thanks
@@chemmuhammad8471 Anytime
I have been trying this method for Vitamin A I am not getting the result exactly for curd...
Sorry for your situation. Try to follow this method strictly. Saponify the sample sample and the standard properly in dark.
Can we use methanol as a solvent in the place of petroleum ether in Gas chromotograp?
Yes. You can
@@MicroChemsExperiments Thank u
Hi could you please share which method like iso or AOAC like?
Sorry. We don't provide any document
Its important to filterd methanol
Yes. It is very important
I would recommed HPLC grade solvents, and the use of fluorescence detection (for vit E exition 280 and emission 325nm, vit A ex 342 nm, em 476 nm) to get much better selectivity and sensitivity.
Hi where can i find your part 1 of video
Please find it from the video list
Hi, first thank you for the video. Can you give me the reference of the standards for Vitamin A and E so that I can buy it
Buy from Sigma Aldrich
@@MicroChemsExperiments yes I know its from Sigma Aldrich, but I need the product number because when I make a search for the vitamin, it gave me several ones, and I don't know which one should I choose
@@aymenogkush
1. Vitamin-A standard: (Retinyl Acetate analytical standard; CAS Number=127-47-9; Product Code: 46958)
2. Vitamin-E (Alpha Tocopherol analytical standard; CAS Number: 10191-41-0; Product Code: 47783)
Thank you very much. And for the filter that you used for the filtration of the methanol, what is the type of the material (PTFE or Cellulose or Nirile or Glass fiber etc..) and the porosity that should I used? If it's possible the product number
@@aymenogkush Cellulose membrane filter. Look up to the video. Details specification is seen when using the filter peper in this video. Product number is not avaulable for this product. You can purchase this from the local market.
Also the mobile phase prepared here is applicable to any vitamin A column?
No. Only applicable with C18 column
please provide informatio, How should we prepare stock solution, for Vitamin A
Saponify your standard and make the final volume of 10 or 100 ml with methanol.
For more details, mail us
@@MicroChemsExperiments Thank you
Hello, Instrumental analysis for vitamin D?
Noted
Where is the video for instrumental analysis of vitamin d?
What is the column your using here please mention that
Watch this video again. All the details is given in this video
@@MicroChemsExperiments I am using hypersil gold column
@@aswinkumar8016 Did you get the vitamin A standard peak?
@@MicroChemsExperiments Yes, but the value is very high when compared to the label value
That's why I am asking ur using normal hypersil gold column or acclaim 120 PA / C18 column
Filtering MeOH, sonicate it and then putting inside a piece of cork, bad techniques and sources of contamination are dressed here as an expert advice in just 3 min.
I kept watching the video and now I understand that peak tailing, you're destroying your instrument, mister. Worst of it all is that you must be teaching people both in real life and through your videos, I'm sorry for them.
@@vsolomtn peak tailing makes more variation in the results as the proper integration of tailing peaks is not easy
@@velimattiollilainen Peak tailing is caused by different migration speeds of the analite inside the column, which indicates a poor performance of the system. Areas indeed will be different because because that method isn't suitable for any kind of separation. It can't be a matter of playing the edges on a latter integration when you have no reproducibility to start with.
Noted
Thanks you, i like channel, you are so professional
Thank you
Sorry I'm not understand about the standard preparation. Can you explain it?
You can mail us explaining the problem.
Write us at mail.mic.chem@gmail.com
Both are very informative & helpful video.
Glad you think so!
Where is vitamin d analysis procedure?
Will upload later
what an video on vitamin, Great video and very clean each step and very knowledgably,
For Vitamin D analysis using hplc instrumental analysis?
I want to say calculation method for viramin A and E by HPLC
We don't know about the method you are talking about.
@@MicroChemsExperiments How can we calculate the content of Vitamin A and E in our Sample?
I mean ....Please share calculation formula
please share calculation formula.....I'm waiting
Thank you so much for this very detailed and informative video. This is a great help. Also would like to ask if you have video for analysis of water soluble vitamins.
Coming soon!
Which hplc model used for this test? Please share the standards preparation link
Model: 3000 Ultimate.
We will make video for standard preparation later
Why do you set run at 30 min while RT of vitamin E is about 8 min
For proper column washing. You can set the run time at 20 min.
How to test the gamma origin of Reich Bran oil sir
Do you mean Gamma Oryzonal?
This extracted sample preparation method can use all sample or specific ex. snack fruit vegetables
All samples
Can you share the range of std curve that you used .
@@Nicha-qu3nw 5 10 20 50 ppm
Thank you a lot, you deserved a medal
Thanks. I just received one medal from you.
Where you buy this standard Vitamin ?
You can buy standard from Sigma Aldrich
@@MicroChemsExperiments and please tell me about the vitamin b sample presentation
@@gauravkamble4085 we will make video later
Waiting
What are you waiting for?
@@MicroChemsExperiments For more experimental video. Specially for hplc
Thank you for your patience. We will be here with many more videos about HPLC. Stay with us. Share this group to support us.
@@MicroChemsExperiments the videos you provided are really fantastic. It attracted my attention due to Covid-19. I was share your Videos to my students.
@@rajuahmmed1257 Thank you so much for your support.