Protein analysis: Western Blotting

[@coralyscintroncintron8131]

Cool video!

[@BioLabCollective]

Thanks for dropping by 🙌

[@jayzeeeasy4734]

Wouldn’t the tertiary structure of a target enzyme like LDH be broken down during SDS into strands of amino acids bound to SDS therefore disallowing the relevant immunological markers to bind? I assume the markers can only bind to enzymes in their natural structure, is this so?

[@BioLabCollective]

You are right, that is why not all antibodies are suitable for western blotting. However many antibodies are raised to recognise small portions of proteins, which can be in their denatured form.