Couldn't the restriction enzymes also cut 3*1 Kb instead of a single 3Kb? How could you know? On a side note, this lecturer lectures me and she is such a great teacher!
Thank you so much! I was beginning to get confused cause another example in my book uses 4 RE so it was getting hard to keep track of them, especially when it was the second example
Distance or position involves 'kb' which is the number of 1000x bases involved in each fragment of 1 DNA sample(size). The number of lines you see in each column is the number of fragments there are in 1 DNA sample which simply says how many sites are involved. by looking at the single digestion, you can add the positions up to get the total number of 1000xbases involved in your 1 DNA Plasmid sample.
she's wrong, its actually because there is a total number of bands which are determined theoretically from the single digestions, you don't know the amount of kb(size) of each fragment though until you run it through the gel BUT you do know the total number of fragments. You have to picture the process mentally. So the first enzyme splits into one big fragment(1 site), then if you combine the other single enzyme which you know has 2 fragments(2sites) you get a total of 3 sites. that makes a total of 3 fragments. if you have 4 kb1 strands then you have a total of 5 fragments which would not make any sense with your theoretical mapping which you are hoping to find 3 fragments. the only possible way to get three fragments there would be 1 overlapping fragment of 3kb size.
I just love the way you teach , slow, sure and with well organized explanation and you are indeed love what you do. God bless you abundantly.
Thank you so much! This really helped me understand restriction mapping starting from the basics.
:)
Thank you so much Professor lady. You're great!
Thank you isn't enough for the efforts that teachers like you put in order to educate us. ❤
thank you so much! helps me a lot
This was such a great explanation! Thanks so much!
Excellent vid
Great video!
Thank you so much!
I like this lesson.You are a good teacher. Thank you for the educational upload! ;)
Thanks for explanation! My concepts are cleared now!
This is so great for my A2 classes - thank you
very helpful! thank you so much!!!
Thank you for explaination it's cleared now!
Couldn't the restriction enzymes also cut 3*1 Kb instead of a single 3Kb? How could you know? On a side note, this lecturer lectures me and she is such a great teacher!
What is her name? I want to become a student of her.
Thank you so much!
I was beginning to get confused cause another example in my book uses 4 RE so it was getting hard to keep track of them, especially when it was the second example
Thanks for making this so simple
You are a genius in teaching !!!
Such a good explanation thank you so much!!
excellent video. The only downside is the ring on the finger.
Im sort of asking because s1 nuclease and primer extension assays have confused me as to which type of DNA is run on the gel.
awesome video
i wish you were my professor
extremely helpful. thank you.
i love you u are awesome
Very useful and so easy 😊 .. thank you
Explained in a very easy method
Very well demonstrated thankyou...I just wish you had time to do another couple of examples....
:( this series of videos are very good, so sad you stopped
many thanks dr Helen
I understand this much better now. My teacher is confusing me though by drawing a straight line and mapping it... I don't get it.
This may seem like an odd/silly question, but when you run the fragments on a gel?? Are they run as ssDNA or dsDNA??
+MsTommyknocker usualy they ran denatured = ssDNA but also depends what techniques you use
excellent explanation mam, please make video regarding dna finger printings related problembs
What is your own website please?
Can we find it on RUclips in written form
please go on taking videos!
what about distance migrated?
Distance or position involves 'kb' which is the number of 1000x bases involved in each fragment of 1 DNA sample(size). The number of lines you see in each column is the number of fragments there are in 1 DNA sample which simply says how many sites are involved. by looking at the single digestion, you can add the positions up to get the total number of 1000xbases involved in your 1 DNA Plasmid sample.
There is a problem with the sound
What's your name, Professor ?
Does anyone know this beautifull girl name?
Why can't there be 4 1kb bands?
+avraham raichman it's because you don't get any band at 1kb at the agarose gel that we're shown here
she's wrong, its actually because there is a total number of bands which are determined theoretically from the single digestions, you don't know the amount of kb(size) of each fragment though until you run it through the gel BUT you do know the total number of fragments. You have to picture the process mentally. So the first enzyme splits into one big fragment(1 site), then if you combine the other single enzyme which you know has 2 fragments(2sites) you get a total of 3 sites. that makes a total of 3 fragments. if you have 4 kb1 strands then you have a total of 5 fragments which would not make any sense with your theoretical mapping which you are hoping to find 3 fragments. the only possible way to get three fragments there would be 1 overlapping fragment of 3kb size.
Upload chromosome walking elaborately ...mam