Nice explanation, simple, interesing and understandable, thank you) it would be nice if you also told the different enzymes and their features that are responsible for cutting DNA
Restriction enzymes have names and they are unique in a way that they can only recognize and cut a specific DNA sequence. Think of them as molecular scissors that seek out and cut only at a specific pattern. So if your unknown sample has the sequence that the restriction enzyme can recognize, it will be cut. If it does not have it, it will not be cut. If your linear DNA sample has 1 restriction site, it will be cut once and you will get 2 separate fragments. If your linear DNA sample has 3 restriction sites, it will cut 3 times and you will get a total of 4 fragments. To find out how many base pairs these fragments have, you will then run them through gel electrophoresis. :)
@@4EvaBio amazing! thanks for replying to me.. I am having trouble getting a well defined bands for my digestion product, I can see some separation but it is quite faint.
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This is SO helpful, better explaining than my teacher thank you so much!!
Brilliant explanation. Thanks for sharing. Perfect for explaining DNA Profiling.
THANK YOU! You helped me out alot on my assignment. Tytytytytyty
Just what I needed.
Amazing explanation!
Nice explanation, simple, interesing and understandable, thank you)
it would be nice if you also told the different enzymes and their features that are responsible for cutting DNA
Thank you very much
Great explanation!
Thanks
Very well explained, if I have an unknown sample, how do i know at which site will they cut and how many bp they will produce?
Restriction enzymes have names and they are unique in a way that they can only recognize and cut a specific DNA sequence. Think of them as molecular scissors that seek out and cut only at a specific pattern. So if your unknown sample has the sequence that the restriction enzyme can recognize, it will be cut. If it does not have it, it will not be cut. If your linear DNA sample has 1 restriction site, it will be cut once and you will get 2 separate fragments. If your linear DNA sample has 3 restriction sites, it will cut 3 times and you will get a total of 4 fragments. To find out how many base pairs these fragments have, you will then run them through gel electrophoresis. :)
@@4EvaBio amazing! thanks for replying to me.. I am having trouble getting a well defined bands for my digestion product, I can see some separation but it is quite faint.
Thx
Wonderful and informative videos. Thank you!
thanks a bunch ma'am❤
You're so welcome! :)
How many fragments formed in GAATTC sequence
2 fragments
and each fragment consists of 2 DNA strands. :)
@@4EvaBio thanks
Can you make genetic scissors technology at home? And can you make a deaf cat hear?
Thank you very very much