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Restriction enzymes have names and they are unique in a way that they can only recognize and cut a specific DNA sequence. Think of them as molecular scissors that seek out and cut only at a specific pattern. So if your unknown sample has the sequence that the restriction enzyme can recognize, it will be cut. If it does not have it, it will not be cut. If your linear DNA sample has 1 restriction site, it will be cut once and you will get 2 separate fragments. If your linear DNA sample has 3 restriction sites, it will cut 3 times and you will get a total of 4 fragments. To find out how many base pairs these fragments have, you will then run them through gel electrophoresis. :)

@@4EvaBio amazing! thanks for replying to me.. I am having trouble getting a well defined bands for my digestion product, I can see some separation but it is quite faint.

Check out my other video for more details to help you understand restriction enzymes, how they cut DNA and how DNA fragments run through the gel electrophoresis :) ruclips.net/video/2J5DSFCdgj8/видео.html

@@4EvaBio no I mean ..can you make a vedio on how we can detect homozygositt and hetrozygosity in bands while studing genetic diseases

Simply put.. Homozygous alleles(genes) show up as 1 band on the gel because they are the same size and run together through the gel. Heterozygous alleles are different... so they may have different sizes, hence show up as 2 separate bands. :)@@misbahfarooq1189

unfortunately it cannot regenerate its lost part from small pieces made by a mixer .it has only some regeneration power

Yes, of course. Thank you for your kind words.

​@@4EvaBiomam so can you give me your Instagram Id

Hi there! Thank you so much for your kind words! I'm thrilled to hear that the video has been helpful for your studies. Sending lots of love back to you in India!❤

@@4EvaBio thanku mam. I m now studying in class 11th and also preparing for medical entrance. And mam my name is Rahul Mazumder. Mam I have a question? Where are you from mam?

That's wonderful, Rahul. Best of luck with your studies. :) I live in Texas, USA, but was born in Europe. @@rahulgamer2324

​@@4EvaBio thanku mam.mam I m so lucky that suddenly I found your a short video about planaria and from then I saw your some videos and now I am became your fan.you are a amazing bio faculty and explanation is too good. mam I have a reques.when I study biology then sometimes some doubts are arise.but I can't not solve properly.so I decided to ask doubt to you in Instagram because in youtube my message are too late to reach you. so mam if you help me I will very happy.

You can e-mail me at 4EvaBio@gmail.com

So glad to hear that. :)

Thank you :)

I haven’t done that in my lab, but I would assume it’s definitely a possibility.

According to the video it is possible that they can develop 2 tails, I have been searching for awhile and I haven't found any videos of it, is it possible if you can make a video on a planarian having 2 tails? I'm really interested in them and how they work.@@4EvaBio

I can certainly try but we work with them in my class once a year... and so far, every time we cut the tail, it fused back together. So that's definitely something to explore and research further. Also, they are very small and our microscopes aren't the best... so making precise cuts is definitely a challenge. :) @@DecaS3

Ouch! Most likely not.

​@@4EvaBiofr

So glad I could help <3