you didnt show the place where the 1550 and 1450 fragment shoud have been added to the plasmid....you made two plasmid on with only Enzyme 1 and 2. can you please add it to the plasmid
Awww.. looks like you missed it. Ok, remember a specific restriction enzyme only cuts at a specific DNA sequence. So mix your DNA with that enzyme, and you will find the cut- the location. If you have more than one enzyme, you can build "a map" of restriction sites- cuts. Here's an analogy: take a circular string, cut it into different length pieces, then try re-assembling the same circle... like a puzzle. The location where the pieces meet- is the location where the specific enzyme cuts.
a very great job
This was very helpful!
Thanks a bunch 🌟🌟
you didnt show the place where the 1550 and 1450 fragment shoud have been added to the plasmid....you made two plasmid on with only Enzyme 1 and 2. can you please add it to the plasmid
this was very helpful thank you so much!!
Thank you so much... This video helped me solve a problem I've been battling with... Wonderful Job🎉
So glad I could help
What if E2 also gives two fragments. How can we do the mapping then?
Wow thanks 👍🏻👍🏻
You’re welcome 😊
you did not add enzyme 3 to the final map, is there any reason for that?
Not sure what you mean. Enzyme 3 is definitely there :)
You didn’t show how to find the specific location of the restriction enzymes
Awww.. looks like you missed it. Ok, remember a specific restriction enzyme only cuts at a specific DNA sequence. So mix your DNA with that enzyme, and you will find the cut- the location.
If you have more than one enzyme, you can build "a map" of restriction sites- cuts. Here's an analogy: take a circular string, cut it into different length pieces, then try re-assembling the same circle... like a puzzle. The location where the pieces meet- is the location where the specific enzyme cuts.