Restriction mapping of circular DNA

#restrictionmapping #hotosolverestrictionmappingquestions #geneticmapping # circulardnamapping #restrictiondigestion #restrictiondigestionproblems Learn #withme
#hindiii #banhi #ecor1 #fragments #digest #hpai
A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes. In molecular biology, restriction maps are used as a reference to engineer plasmids or other relatively short pieces of DNA, and sometimes for longer genomic DNA
In this video, you will understand how to create restriction maps of a circular DNA

[@ritadukunde5176]

I should have watched this earlier before I started crying...the video was really helpful
Thank you so much

[@BioMagica]

Hahaha. Happy to help you out dear 😊😊😊

[@aminahassan9599]

you have just saved my life after 1 hour of desperate searching in my way too expensive and unuseful literature, thank you!!

[@BioMagica]

Happy to help 😊😊😊

[@poobeni]

CEO of restriction mapping🔬🧬

[@BioMagica]

lol. Thank-you dear

[@sara95599]

Thank you so much, this video really helped me. I'm studying for my Molecular Biology exam and now I'm a little bit less scared :)

[@BioMagica]

That's great to know. So happy to help 😊😊😊

[@mrjoseph2105]

30 minutes before my exam ur a lifesaver maam

[@BioMagica]

Happy to help 😊😊😊

[@hellohari85]

Thank you very much mam, I could have never understood restriction mapping without watching this video, thank you spookily much. It helped me a lot

[@BioMagica]

😊😊😊

[@jameslowe2342]

wow. I'm confident about these problems now. The concept is so simplified. thanks

[@BioMagica]

Thanks :-)

[@jameslowe2342]

BioMagica Please make more videos. you are great

[@hemantsajwan5246]

Best video . And very helpful

[@BioMagica]

Happy to help 😊😊😊

[@saraisteenson7413]

so much better than my genetics professor, you are amazing!!!!

[@BioMagica]

Thank you. Happy to help 😊😊😊

[@ka1458]

Thaannkkkk yoouuu varyy vaaryyy muuucchhh♥️♥️♥️♥️

[@BioMagica]

Happy to help 😊😊😊

[@Mielste]

Merciiiii beaucoup vous sauvez mon année (you save my year) 💕💕💕

[@BioMagica]

Glad you liked it. Do share

[@fortunatem3378]

This video was helpful thank you

[@BioMagica]

Happy to help 😊😊😊

[@chelseyl834]

very helpful thank you sooo much

[@BioMagica]

Happy to help 😊😊😊

[@noorulnaeem8603]

Good Job maa'm..Thank u soo much...May Allah Bless u....Aameen...

[@BioMagica]

Happy to help 😊😊😊

[@beverlyselman8487]

Thanks this video clarified my mind confused mind. I appreciate thanks

[@BioMagica]

Happy to help 😊😊😊

[@ahmarrauf2108]

explained a difficult topic in a very lucid manner, much appreciate

[@BioMagica]

Thankyou ☺️

[@sudiptasarkar5520]

Thanks for this video

[@BioMagica]

Glad you liked it. Do share

[@Eru170]

thank you miss, your video save my life for tomorrow final exam

[@BioMagica]

Happy to help 😊😊😊

[@Gaana_madhu]

I understood it very easily ..before it was running all over. thankyou it was very helpful

[@BioMagica]

Happy to help 😊😊😊

[@wargecko2238]

this video helped alot i watched some other this was the only one i understood thankyou for breakin it down simply.

[@BioMagica]

So happy to know that it was useful to you

[@akeel77]

Thanks, this helped so much.

[@BioMagica]

Happy to help 😊 all the best 👍

[@BioMagica]

Do share 😇

[@LaDyAXE1204]

So helpful! Thank you!

[@BioMagica]

Happy to help 😊😊😊

[@sandaligamage1143]

Thank you so much. It was very helpful. ❤

[@BioMagica]

Happy to help 😊😊😊

[@mitchmitchell305]

Wow ! Very helpful thanks

[@ellie9316]

Thank you for making this make sense

[@BioMagica]

Happy that it does 😇 do share 😊

[@youngsavagekaeJ]

Very clarifying video. Will watch once more!

[@BioMagica]

:) thanks

[@clazoidberg9647]

Thank you!

[@BioMagica]

Happy to help 😊 do share 😇

[@alhaeri1]

great stuff

[@BioMagica]

thanks

[@Owssey333]

Thank you so much this has really helped me understand restriction sites for my exams!

[@BioMagica]

😊

[@ornelanono3401]

Thank you soooo much for this video!!!!

[@BioMagica]

ornela nono Happy to know it was helpful ^_^

[@rupeshprasadmishra8861]

Exceptional thank u ma'am jai jagannath

[@BioMagica]

Thank you too

[@ghadamahmoud4122]

It was very helpful ,thank you so much 😊😊

[@BioMagica]

Happy to help 😊 do share 😇

[@faizaansari8981]

U Really simplified the concept in a short video. Thank you so much 😃😃😃😃

[@BioMagica]

Glad you liked it. Do share

[@nancymalik3912]

Damm easy well done mam

[@BioMagica]

Glad it was helpful 😊

[@channelouma3306]

Best video ever ❤️❤️❤️❤️❤️❤️❤️ ty soooooo much

[@BioMagica]

Welcome

[@babusalam777]

awesome explanation. you are the best :)

[@diannanaheral4727]

very helpful for my cellmol, thanks my dear!

[@emmamakishti4710]

Thank you for this video! You clarified a lot of things I previously didn't understand 😊 keep up the good work!

[@BioMagica]

Happy to help ☺️

[@anjaliyadav-df4ow]

Thank you soo much... You come as savior for students like me... Thank you soo much.

[@BioMagica]

Happy to help. Thank you 😊😊

[@quicksports347]

Nice...

[@BioMagica]

Glad you liked it. Do share

[@joshr.5199]

Bless🙏

[@BioMagica]

Happy to help 😊

[@karmachemebhutia5402]

Thankyou so much✨💛

[@BioMagica]

Happy to help 😊 Do share with others.

[@sonemanibitna3053]

THis is great

[@BioMagica]

Glad you liked it. Do share

[@thaibinhnguyen6333]

Your video and explanation save many students’s life

[@BioMagica]

Thankyou so much ☺️🤓👍

[@ImranAnsari-ng3vo]

Nice video. Very helpful.

[@BioMagica]

Glad you liked it. Do share

[@keerthanam1301]

This video ws really helpful thanks alot

[@BioMagica]

Welcome 😊

[@user-fz1tl2dh9b]

THANKSS MAM 😍😍💥💥💯🙏🙏

[@dr.kumarmanish8359]

great one

[@BioMagica]

Thankyou 😊

[@gajendersingh5960]

Nic plz upload all these lecture

[@BioMagica]

Glad you liked it. Do share

[@lauriev7453]

video was helpful for last minute understanding of this before my quiz tomorrow thank you

[@BioMagica]

Glad it helped! And all the best

[@shiwanimaurya3147]

Thank you for such a clarifying video❤

[@BioMagica]

Glad it was helpful!

[@manamichakravorty6114]

It was so helpful!! Thank you so much ❤❤

[@BioMagica]

Happy to help 😊😊😊

[@BioMagica]

Do share 😇

[@kanicasharma1105]

thank you so much for such crystal clear explanation.

[@BioMagica]

Happy to help ☺️

[@pratibhasingh269]

Can't thank you enough

[@BioMagica]

Happy to help 😊 all the best 👍

[@gaurisha-np3bp]

Very nice ❤

[@jessicawulfsohn4602]

SO GOOD THANKYOU

[@BioMagica]

happy to help. Do share

[@tadrosrafikyoussefrezkrasl9641]

THANK YOU

[@BioMagica]

Glad you liked it. Do share

[@ryanbuchanan3273]

The music in the video ws relaxing...

[@BioMagica]

Thankyou

[@talaliqbal3895]

nice video

[@jayawewa4358]

thank you. Very useful

[@BioMagica]

You are welcome!

[@BioMagica]

Please do share the video

[@PoojaSingh-xe5od]

Helpful

[@emriyekaya1296]

Wunderschöne 👏👏👏👏👏👏👏👏

[@BioMagica]

Thankyou ☺️

[@shahidrasool9791]

Thanks a lot

[@BioMagica]

Happy to help

[@aaronanil6829]

Really helpful. Thank you so Much!!!

[@BioMagica]

Happy to help ☺️

[@kshitijsomwanshi4888]

thank you so much

[@premroy8312]

Nicely explained.
Don't know why but it seems u made this video when u were in college.

[@BioMagica]

Well partially correct. I made this video while i started teaching in college. It was only a year after i completed my masters, so quite close.

[@BioMagica]

Happy to know it was useful 🐱

[@hrushikeshvardhan3626]

Thank you so much h❤❤❤

[@nitikasharma6557]

Thanku soo much

[@BioMagica]

Most welcome 😊

[@kirak7789]

Ty

[@BioMagica]

Happy to help 😊

[@tunetonic04]

Thank you so much❤

[@BioMagica]

You're welcome 😊

[@prokashray7599]

Thanks mam

[@BioMagica]

welcome

[@NYCCarrie]

If you have English subtitles, it will be perfect, because the accent is very heavy, which is a bit difficult for people with poor English hearing. Anyway, Thank you so much!

[@BioMagica]

Yes i get it. I have uploaded subtitles on most of the videos. Trying to add for all soon 🙂

[@atharvakulkarni8818]

Thiss video is really nicee. Please make a video for mapping of linear DNA as well.

[@BioMagica]

ruclips.net/video/HZKMzixy1ek/видео.html

[@BioMagica]

Thankyou. Hope this helps

[@jagkarkumar]

Thanks

[@BioMagica]

Welcome

[@adrijabanerjee-ek6hw]

Thank you so much for this❤ it helped a lot.

[@BioMagica]

You're so welcome!

[@AbhiSinghmp]

Thanx 😊

[@minakar2144]

Thank youuuuu

[@sairamesh8594]

😍

[@BioMagica]

😇

[@lucastan5707]

when ur teacher takes a whole lesson to explain this concept and u take 6 min

[@BioMagica]

lol.Thankyou. Glad it was useful to you. do share

[@henributler9324]

How can you do this if one of the digests has 2 fragments, and the other 3

[@user-wr2pd3jd5w]

its confusing what if i have EcoRI cut in 2 places and BamHi cut in 3 fragment how am i supposed to do i only end up getting 1 ecori in the circular dna

[@BioMagica]

If you have 2 ecori and 3 bam hi you will have total 5 cuts meaning 5 fragments..... normally in problema you will have atleast one enzyme which is making one cut...but even in the condition that you gave, you can randomly place the 2 ecori sites on the circular DNA....and then based on the double digestion fragments...you can mark the three bamHI sites.

[@BioMagica]

Try referring to a basic video..I'll add a link here

[@BioMagica]

ruclips.net/video/HZKMzixy1ek/видео.html

[@BioMagica]

If you still have doubts. You can email the particular problem at [e-mail]

[@user-wr2pd3jd5w]

Thank you very much I'll try that right now

[@hellohari85]

Sooooooo* much

[@BioMagica]

Glad you liked it. Do share

[@epsilon47]

But how do you know the order of the 6/4/3 fragments?

[@BioMagica]

we just randomly place them ensuring that the fragments obtained are correct. In some cases there are be more possibility for the fragment placement. Actual order can be known by sequencing.

[@josa5463]

Hi ,If I want to learn restriction mapping by myself how can I learn it ?

[@manarabual-rub8458]

Plz can you help me in relative problems ?

[@BioMagica]

Yes sure

[@manarabual-rub8458]

@@BioMagica how can I connect with you ?

[@manarabual-rub8458]

@@BioMagica plz replay to me

[@BioMagica]

@@manarabual-rub8458 [e-mail]

[@patriot1531]

What if you are combining more than one map? Help would be appreciated!

[@BioMagica]

Can you send an example so it would be easier to help you out. You can email to [e-mail]

[@gokulmalwe3177]

If you have a circulation plastic containing a single ECORI site and you cut it with ECORI how many pieces of DNAwill be formed?what about circular plasmid containing ECORI Sites.?What about linear piece of DNA containing one ECORI Site

[@BioMagica]

Please refer to my video which explains the basics. In circular DNA u get one piece for one site and if the DNA is linear then u get two pieces with one site

[@BioMagica]

ruclips.net/video/HZKMzixy1ek/видео.html

[@gokulmalwe3177]

Thanks mam you are very nice in nature

[@balentine]

I cannot understand what you are saying... i need subtitle

[@BioMagica]

Sure. Will add subtitles asap.

[@chakerzeine]

16/05/2023

[@c4_collide]

Bits pilani students attendance lgao 😂

[@arindamchowdhury5944]

copied from Shomu's Biology xD

[@BioMagica]

Anyone who knows how to solve it will definitely think in this direction 😊😊😊

[@SP-nt9nm]

@arindam This video is so much better, and simplified. Well obviously the concepts will be the same. But the content is consized and easy to follow.

[@SP-nt9nm]

@@BioMagica I agree. Thank you for making these videos. They are to the point and very easy to understand. Keep up the good work

[@Ijazalijin]

thanks a lot, it really helped me

[@BioMagica]

Glad it was helpful 😊