Restriction Digestion of DNA

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  • Опубликовано: 15 окт 2012
  • For more information, visit www.bio-rad.com/yt/lambda-dna.
    This video demonstrates how to set up a restriction digest using the Bio-Rad Restriction Digestion and Analysis of Lambda DNA kit. Techniques for setting up a restriction digest using a micropipet are shown.
    www.bio-rad.com/evportal/desti...
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Комментарии • 20

  • @beanheadr
    @beanheadr 4 месяца назад +3

    Interesting that this process has not changed much over the years. I did a high school internship at the Kennedy Krieger Institute back in the late 90's working with X-ALD with mice as our catalyst. I remember being nervous using ethidium bromide after my lab director explained that exposure to it could cause my DNA to mutate!

  • @arseniipavlenko1048
    @arseniipavlenko1048 3 года назад +83

    So no one is gonna mention that the tubes spell out HELP at the end?🤔

    • @neil1988
      @neil1988 2 года назад +1

      HAHAHA 🤣

    • @esther9223
      @esther9223 Год назад +1

      First thing I noticed🤣

  • @nbent4607
    @nbent4607 3 года назад +6

    Really helpful, thanks a lot!!

  • @saranyasakthivel5071
    @saranyasakthivel5071 9 лет назад +5

    it is very useful to understand

  • @garimaupadhyay3469
    @garimaupadhyay3469 3 года назад +3

    whats the name of the buffer???

  • @siddharthsrivastava8652
    @siddharthsrivastava8652 10 лет назад +8

    Video on calculations that go into setting up such restriction digest
    ruclips.net/video/eth9Mh6Nsu4/видео.html

  • @angeliebacon8276
    @angeliebacon8276 2 года назад +3

    never seen green equipment 😍

  • @Han-cp9ds
    @Han-cp9ds 8 лет назад +2

    what do u mean by corresponding tubes for the different restriction enzymes? this video was informational!

  • @arresteddevelopment2158
    @arresteddevelopment2158 11 месяцев назад +1

    Will I ever get to do this again? It was fun. Sigh

  • @rajubharti8881
    @rajubharti8881 5 лет назад +6

    Is restriction enzyme added in L tube or only in E, P, and H tube ? make it clear .

  • @jiminslonglostgrapejams2582
    @jiminslonglostgrapejams2582 6 лет назад +10

    Bts dna