Isolating Plasmid DNA

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  • Опубликовано: 26 дек 2024

Комментарии • 155

  • @ruttgerr22
    @ruttgerr22 10 лет назад +43

    This is fantastic!! In 10 minutes, you've managed to explain something that my professor hasn't been able to do in an entire semester. Thank you.

  • @ambinatrix
    @ambinatrix 13 лет назад +1

    "...remember to balance the centrifuge!" #1 message from this video! Great job everyone! I love the triumphant music and narration at the end - the plasmid DNA is isolated!

  • @johnagbo20
    @johnagbo20 7 лет назад

    What an exquisite way to explain the process of plasmid DNA extraction from a bacteria, the best I have seen so far.

  • @johannaamunjela2750
    @johannaamunjela2750 9 лет назад +30

    You are too awesome Katie. The process is very clearly and enthusiastically explained - great animations too! Thank you for this it has been very helpful.

    • @ecofilmkt
      @ecofilmkt  9 лет назад +4

      Johanna Amunjela Thank you so much! I am glad the video was helpful and you enjoyed it.

    • @erin.mcguire
      @erin.mcguire 9 лет назад +1

      +Katie Wolfson amazing, they should include a link to this video on the QIAGEN kit :)

  • @danishfelipe
    @danishfelipe 6 лет назад +3

    This is by far the best lab video I've ever watched! Usually all the vids I find are really boring. Thank you for making it interesting

  • @rehabalsaleh166
    @rehabalsaleh166 4 года назад +1

    Amazing! I usually spend at least 72 hours of research to understand the purpose of every step explained here because of my failing professors and TAs.

  • @Shazi5886
    @Shazi5886 Год назад

    This video definitely needs thumbs up... easy and straightforward steps...

  • @rosalizarraga3433
    @rosalizarraga3433 11 лет назад +1

    I was totally lost while rushing through this procedure in lab. This video explained everything so clearly! Thank you!

  • @ahmedg.hussain9699
    @ahmedg.hussain9699 5 лет назад +1

    Still relevant till today, thank you all.

  • @jackman00110101
    @jackman00110101 6 лет назад +1

    the music really gives the forefront of human ability a different vibe. A+

  • @78kurenai
    @78kurenai 9 лет назад +1

    I think this is great educational video out there. It shows simple process at first, and then goes over actual process explaining what is going on in detail. I find this really helpful.

  • @evasadat4356
    @evasadat4356 4 года назад +1

    A fun and educational video describing the steps of mini-prep in perfect detail. Thank you so much for this wonderful resource!

  • @WaveRosee
    @WaveRosee 6 лет назад +2

    This was one of the most helpful videos I've watched on RUclips. Thank you so much!

  • @adrianb.4040
    @adrianb.4040 3 года назад +2

    I really must stop and say that this video is so amazing, and I love it! I just would like to be more prepared to perform this in the lab for the first time, and this really has helped me to visualize the procedures! Thank you very much for your superb video!!!

  • @Nvrloptimism
    @Nvrloptimism 10 лет назад

    by far the best on miniprep outline, explanation. This was an excellent video.

  • @lametalk8388
    @lametalk8388 11 лет назад

    I have been searching the whole net for the for the rationale behind each steps and most of them are just steps without the theory behind. This is a great video. Helped me a lot. thanks!

  • @JaPaNesEdropout
    @JaPaNesEdropout 10 лет назад +7

    THANK YOU FOR HELPING ME UNDERSTAND; your clarity and simple animations really helped me make sense of the whole practical aspect :'D

  • @whateverdude789
    @whateverdude789 12 лет назад +1

    great video! couldn't have made it any clearer than this. had to leave a comment 'cause you guys did a great job!

  • @dmsfid7205
    @dmsfid7205 12 лет назад +1

    This video is very nicely done! I get the principle behind the technique now. Thank you for sharing this great video!

  • @Lelouchie
    @Lelouchie 3 года назад

    Honestly the only video that explained it good, thanks!

  • @koisty01
    @koisty01 12 лет назад +1

    That was so great! I study Pharmacy in Scotland and we're doing this on Monday and writing a report on it at some point so that was very helpful, thanks! :)

  • @river7837
    @river7837 4 года назад +1

    Very good video. Easy to understand and informative! 🌟

  • @brandyb9527
    @brandyb9527 9 месяцев назад

    This is a nice refresher. I haven't done a mini prep in years. 😅

  • @ave123ization
    @ave123ization 3 года назад

    THANK YOU, you just saved my academic career

  • @imanbtabar1059
    @imanbtabar1059 8 лет назад

    Great video Katie...you've put in a lot of time for it. Excellent resource for undergrad class. Much appreciated.

  • @lcl1129
    @lcl1129 5 лет назад

    I was searching this video all years long, extracting is mastered now. I DO NOT WANT TO SEE OTHER DISTURBING VIDEOS.

  • @myannaolsen
    @myannaolsen 11 лет назад

    probably the best mol bio technique video I've seen. well done!

  • @significantmatters8740
    @significantmatters8740 3 года назад

    Amazing. You are so awesome! I wish every teacher are like this.

  • @kuchizoundpollution5
    @kuchizoundpollution5 8 лет назад

    Thank u! U r a lifesaver! I'm a research tech nd was freaking out but I'm now sure I'll do it right tomorrow!

  • @nathanielscreativecollecti6392
    @nathanielscreativecollecti6392 5 лет назад +1

    Nice job on the video. The explanation was clear and concise. I would drop the music though. It was distracting.

  • @brittanypena2163
    @brittanypena2163 7 лет назад +1

    ABSOLUTELY SO HELPFUL! Thank you so much for this!

  • @xuhangwu1770
    @xuhangwu1770 4 года назад

    Super easy to understand when you put this way!!! Thanks

  • @kaeseebourne
    @kaeseebourne 3 года назад

    I actually enjoyed watching this video. So cute and informational!!!

  • @naginakhan1ify
    @naginakhan1ify 5 лет назад

    I like this thorough explanation step by step in this lab. Good video. Thanks

  • @selveskii
    @selveskii 10 лет назад +1

    Get confused with a miniprep protocol. Thanks for the understandable introduction!

  • @Asmaafahmey
    @Asmaafahmey 9 лет назад

    so nice and clear voice, amazing , explain the technique in a simple way...thanks katie

  • @MrMelbournemarsupial
    @MrMelbournemarsupial 12 лет назад

    Great video - excellent description and the cartoon visuals are just wonderful! Thank you :)

  • @divyamehra5304
    @divyamehra5304 3 года назад +1

    This video is super helpful! can you make one on the western blotting technique?

  • @sighsss
    @sighsss 11 лет назад

    wow, I just took over a project that involves plasmid purification, and this video is a perfect prep for that.

  • @southpawko1991
    @southpawko1991 11 лет назад +1

    Great video . More videos like this that have techniques that we can put on a resume would be good.

  • @SharaPrincessOfPowers
    @SharaPrincessOfPowers 8 месяцев назад

    Thank you, it helps on understanding more detailed.❤

  • @twirlaway568
    @twirlaway568 7 лет назад

    KATIE, ILYSM
    This video was bloody amazing xoxo

  • @connorstimpert3694
    @connorstimpert3694 4 года назад

    Dang, CC's raking in them views! Great video

  • @ademakinwanelson3229
    @ademakinwanelson3229 7 лет назад

    this is awesome. Keep it up Katie

  • @madisondrum9179
    @madisondrum9179 5 лет назад

    thank you! this is the exact mini prep kit we use in the lab !

  • @Sai-fj4wi
    @Sai-fj4wi 8 лет назад

    It was an amazing video. Thank you so much Katie!

  • @killingrang
    @killingrang 12 лет назад

    Thanks for the video, just about to do my first miniprep today and a feel alot more prepared for it :)

  • @ecofilmkt
    @ecofilmkt  12 лет назад

    You're welcome! Glad you like it.

  • @belldushair
    @belldushair 6 лет назад

    this was fun, yes fun. plus i learned a lot without even trying. wow. thank you. this was so helpful.

  • @solomonhan2235
    @solomonhan2235 9 лет назад

    A Life-Saver Video! so sad that my English is not good enough to explain how thankful I am :(

  • @Midea-zl3dp
    @Midea-zl3dp 4 года назад +1

    Sorry but I didn't understand this: For example, potassium acetate lowers the pH. Why does this only renaturate the plasmid DNA while the chromosomal DNA falls off?

  • @nunitchagucci3047
    @nunitchagucci3047 8 лет назад

    Millions thanks to this video!!! You made my day

  • @williamcalero-caceres135
    @williamcalero-caceres135 9 лет назад

    Great explanation! I really enjoyed your video, congrats! Kind regards from Barcelona.

  • @gilbertp5473
    @gilbertp5473 8 лет назад

    Top notch videos Katie thanks so much!

  • @geniex931
    @geniex931 12 лет назад +1

    Thank you so much for this! This is such a great video, the instructions are clear and thorough! Could you please do one on Western Blot as well?

  • @alexbrown8994
    @alexbrown8994 10 лет назад +1

    Thank you so much. this really help me with my lab report.

  • @bsbabla
    @bsbabla 12 лет назад

    Really owesome video. You are a lifesaver. thanks a lot.

  • @TheRowdyranger
    @TheRowdyranger 12 лет назад

    This is the greatest thing ever. THANK YOU!

  • @norahsendi9636
    @norahsendi9636 9 лет назад +1

    thanks a lot Katie, it was very helpful.

  • @summi7628
    @summi7628 7 лет назад

    Omg! Finally everything makes sense.

  • @jfreund5980
    @jfreund5980 Год назад

    Pretty damn good quality video for it being 11 years old wtf

  • @kpoppedwaffles
    @kpoppedwaffles 9 лет назад +1

    I just started doing this in my lab! Super helpful. Thanks so much (':

  • @MrJejayologue
    @MrJejayologue 9 лет назад +6

    Omg great scheme to explain the action of silica. :o Can I use it ?

    • @ecofilmkt
      @ecofilmkt  9 лет назад +2

      MrJejayologue Yes! Please feel free to use it for education. Glad you like it!

  • @oogieboogiedoogie1
    @oogieboogiedoogie1 10 лет назад +2

    It all makes sense now. At long last.

  • @camilasarcone5967
    @camilasarcone5967 9 лет назад +1

    Super helpful video! Thank you so much!

  • @abdulrahmanbiologist3820
    @abdulrahmanbiologist3820 8 лет назад

    It's easy and clearly defined Thank you.

  • @jeansabbat856
    @jeansabbat856 11 лет назад +2

    U'r the best ... going to get an A for my lab report.

  • @chandrakalagowda3129
    @chandrakalagowda3129 8 лет назад

    a very nicely explained video

  • @toastermax1
    @toastermax1 6 лет назад

    Absolutely the best !

  • @sannydavidlumayno9078
    @sannydavidlumayno9078 12 лет назад

    thank you for this!!! this is a very clear instructional video! hurrah!

  • @asinjitdas3113
    @asinjitdas3113 4 года назад

    Absolutely amazing

  • @anamikasharma4930
    @anamikasharma4930 4 года назад

    Thank you Katie.. very simple and innovative demonstration.. can i use this video to explain the protocol to my students...?

  • @muhammad_chugtai
    @muhammad_chugtai 3 года назад

    outstanding video please make other videos, like RNA protein purification.

  • @Min-ep6ff
    @Min-ep6ff 9 лет назад

    thanks for the wonderful concise video, explanation was so helpful in my lab report :)

  • @saowaneewikee1623
    @saowaneewikee1623 8 лет назад

    Hey, Thank you very much for this clip. It is a great video explanation and very useful !!

  • @8303164
    @8303164 8 лет назад +1

    REALLY THANKS. Just a question. How quickly should we work? how much allowable time between the steps is? can I do this procedures for 20 samples?

  • @eeswarannaresh3454
    @eeswarannaresh3454 3 года назад

    Hi Katie Wolfson, great turotial right there. i would like to ask which editing app did you use to edit the video?

  • @nahedhasan9309
    @nahedhasan9309 4 года назад

    Perfect explanation!!!!!

  • @jry2k2000
    @jry2k2000 12 лет назад

    Great video - more please

  • @imranmohd2504
    @imranmohd2504 11 лет назад

    very good explanation thx for nice videos
    can u gide me an idea about eukaryotic cell isolation of dna

  • @gauravresearch8
    @gauravresearch8 4 года назад

    Good video, however, the function of acetic acid was not explained. I was looking for it. Please reply in comment both the function of potassium acetate and acetic acid. Thank you

  • @ecofilmkt
    @ecofilmkt  12 лет назад

    @MrMelbournemarsupial Thank you! Glad you like it.

  • @ecofilmkt
    @ecofilmkt  12 лет назад

    We are planning to produce another video after the holidays. Please let us know if there is anything in particular that you would like to see!

  • @brightstare9011
    @brightstare9011 6 лет назад +1

    Which major to take for clowning

  • @puskaraditya1117
    @puskaraditya1117 7 лет назад

    thank you verry much.
    This is so esay to learn .Thanks a lot

  • @brittanygriggs6776
    @brittanygriggs6776 2 года назад

    This alleviates so much stress cause they gave us a 4 minutes video which didn't even show half of this

  • @CaglayanOZEL
    @CaglayanOZEL 10 лет назад +2

    Thanks a lot. it's very informative :)

  • @TonyKlusacek
    @TonyKlusacek 6 лет назад

    Great job! is very clearly!!! thnks!

  • @bengisumolyer7310
    @bengisumolyer7310 8 лет назад

    Great video!

  • @lynnalradi1526
    @lynnalradi1526 6 лет назад

    what happens to chromosomal DNA during the miniprep plasmid DNA isolation?

  • @shreygupta4113
    @shreygupta4113 12 лет назад

    Excellent Job..

  • @rizaldydanupoyo9443
    @rizaldydanupoyo9443 9 лет назад +4

    Thx alot for this tutorial....

  • @divyabharathy4609
    @divyabharathy4609 11 лет назад

    thank you for such a beautiful descriptions........

  • @danabai852
    @danabai852 7 лет назад

    thank you so so so so much!

  • @martinfederico7269
    @martinfederico7269 7 лет назад

    excellent video

  • @chulin1591
    @chulin1591 11 лет назад +1

    Great video! Keep it coming. :)

  • @espheroz
    @espheroz 9 лет назад +3

    after u done with this process can you start to read the genome sequence of the DNA??

  • @muhammedal-samarrai9498
    @muhammedal-samarrai9498 10 лет назад +1

    fantastic video .. thanks

  • @NamanMalhotra
    @NamanMalhotra 8 лет назад

    amazing and very helpful

  • @clairetz
    @clairetz 9 лет назад

    very helpful video! thank you.