Agarose Gel Electrophoresis, DNA Sequencing, PCR, Excerpt 1 | MIT 7.01SC Fundamentals of Biology
HTML-код
- Опубликовано: 8 май 2012
- Agarose Gel Electrophoresis, DNA Sequencing, PCR, Lecture Video Excerpt 1
Instructor: Eric Lander
View the complete course: ocw.mit.edu/7-01SCF11
License: Creative Commons BY-NC-SA
More information at ocw.mit.edu/terms
More courses at ocw.mit.edu
The fact that this course 7 lecture series is made open to the world for free is in itself revolutionary. This is what open access means. No bullshit whatsoever, just open open. I am a graduating PhD in biomedical sciences and had seen all the course 7 OCW courses when I was an undergrad in a different country. I always share this treasure cove with my mentees in the lab and what's sad is how they take this kind of course for granted. Understanding the foundational principles of how biology and the techniques we use now in the lab work is the first step to anything advanced. And you can actually see how they suffer when techniques do not work, especially because they do not understand the most basic of principles.
Finally I can say I learned at MIT.
I can't believe how easily he explains all the main steps that I couldn't figure out by listening to my professor. I hope he is well respected, because such a passion for teaching is measured in ppb!! Amazing!! Thank you!!
I envy the students who are taking class with this professor, he seems like the kind that actually gives a shit, he hasn't been soul crushed by academia.
Juan Rodriguez Martinez If you give a shit, you dont get sould crushed by academia.
MsTommyknocker you obviously haven't been in rigorous courses to understand what @Juan is talking about.
He is a true professional! Amazing!
Juan Carlos Rodriguez Martinez I am sure of the same
@@forevereveryours yeah, it's made worse if you get involved in university politics, which is abundant and everlasting and a pain in all aspects. It's what ruins most professors
The lecturer is Dr Eric Lander, a pioneer in genetics and quite possibly the smartest person I have ever met. His combination of organization, enthusiasm and accuracy is a wonder to behold.
I wish to meet him also. Thank you for sharing!
I think I'm falling in love with genetics, this man's a genius!
Everyone has already said what im thinking so I'll focus on what few have: this man's awesome chalkboard writing style. Its so graceful and relaxing omg, the mans amazing
Passion for teaching.. What a great guy. MIT is MIT due to people like you. God bless you more
0:28 Analyzing gene
1:09little circles of DNA has diff bii chemical properties electrophoresis
4:27 DNA sequencing
11:25 faster DNA sequencing
Prof. Lander seems to really embrace and enjoy his role as a Teacher. You can tell how passionate he is about teaching... I love when a Professor actually loves his profession and strives to give the best he has to his class.
thıs ıs what you get when u love what u do
This prof. Proves that the way you teach is more important than the knowledge
This professor is amazing! We need more of him around.
wow, this guys knows how to teach. I hung onto every word he said, fantastic energy and explains exceptionaly well !!
He makes this so exciting :) Hes making my lab reports bearable.
This really helped me with a bit on my a-level! Thanks!
Wow!
This is so amazing.
This guy's style of teaching makes teaching all dramatic and learning enjoyable.
the best genetic lesson i have ever seen
I love how he makes complex ideas seem obvious! also the story about saving 4 million dollars was super cool!
Thank you so much! if you're reading this Sir
He's such a great teacher! So much easier to understand.
I've been trying to understand these concepts for PCR with no luck, and I FINALLY UNDERSTAND. Thank you!
Thank you for posting! It's really cool that you use the chalkboard instead of slides. It's helpful to see you go through the steps. Big help! Thank you a lot.
the best lecture in my life. !!! He is the perfect example of the perfect teacher ! ! he does it with so much passion its unbelievable ! sucks that my university didn't have such good professors :(
Damn you left at cliff hanger. Now i have to binge on it.
This shows What makes America GREAT.
You enjoy what you do.
Biggggg thanks to you. I really was going crazy in this topic and i couldn't find out this method until i found this video! This teacher is so smart. Thank you so much for your genetics and science videos❤🙏
marvellous teaching and superb teacher, love to attend each and every lecture :-)
This guy is amazing! Best professor I have ever seen lecture. Makes me wish I was at MIT.
Outstanding teaching.. the students are really very lucky to hear his lecture every time..
He is one of the world's best teachers...
This guy is a fantastic teacher. Keep it up bro!
You are an excellent lecturer. Hope your students understand your great value.
看这个视频搞明白了pcr测序，不错不错，讲解的很不错。超级赞！
This professor is really great, I'm getting his explanation and just sorry I didn't see this before.
No wonder MIT is ..well MIT
Is there any more videos about him. He is a great speaker and the way he presents the material he does it with enthusiam.
he makes this so interesting and really grabs your attention :O
Best teacher in the world! Loved the class.
THIS PROF IS SO AWESOME! learning these subjects from my teacher was really boring since all she does is sit there and talk. The way she teaches makes the subjects uninteresting, but this professors has passion when he teaches, and makes it so much more interesting and fun. Also, I finally understand what I have been struggling with for a while now :)
I agree
É impressionante como as aulas fluem muito bem. Eric Lander is an amazing teacher.
i wish i have a professor like him he deserve all the respect
This professor is so good! Ty so much
Such a great teacher!
Glad I found this vedio, as a chemist his talk made sense to me.
Great tutorial, thank you so much for posting this
A great teacher makes difficulties easy.
I'm an EE major .. but I watched the whole video. Truly amazing teacher.
WOW JUST WOW!!! Thank you Sir. 👏
i thought i will get bored but really it was an interesting lecture
thank you very much for your hard work and you seem to love genetic that why this lecture was interest , and i hope some day i will be like you
Oh my !!! I'm love whit the way he teaches
This was fantastic, great lecture.
Such a great professor.
love this professor!
cool lecturer with nice handwriting in chalkboard. best video explaining AGE so far I have found. thx for upload.
I followed every word. Beautiful.
inspire me...amazing teacher.i'll be as you for future.
Now we can look very fast at the sequence with PacBio or OxfordNanopore instruments :)
Wonderful lecture I envy those who actually attended this lecture in real life and I can say that MIT is the best institute.
Very clearly lecture help me a lot!
MAGNIFICO PROFESOR.
thank you alot that helped alot understand D base
impressing i finally i understood everything!
I really like this class
Lol here I am, watching lectures for fun while working in my COVID lab. Lander makes me miss school, wish professors at UCSD were even a fraction as passionate as him.
I'm super impressed by his handwriting on the blackboard.
YOU TEACH VERY WELL
Really Awesome!!!
Very informative
very good teacher!
Just awesome 👍
So helpful, thanks :)
amazing teacher
Thanks itwas so nice and intresting
He is amazing!!!!!!!!!!
Good teacher !!!!
he is awesome!!
really helpful vid as i've got my biochemistry exam next week. i hate to be 'that guy', but when he draws out the deoxyribose at 14:00, he puts in a carbon at the top point of the pentagon (since every corner = carbon atom), when it should be an oxygen.
Eric Lander!! wooow
Well.. Its a MIT prof so.... Yeah he's way better than our community college profs...
This prof is great! Where is next part?!
Great handwriting for a teacher
fantastic
Awesome!!
this guy's great
A great teacher :D
just awesome ;)
my life's much easier now ! Thanks lol
:)
Amazing!
I LOVE that he doesn't use powerpoint.
lol, it's kinda "cute". cute and Nobel prize worthy.
better explained than any lecturer at king's college london.... shoulda gone to MIT
Awesome
God bless you sir. Envy those students
i'm watching this in 2018 and finally i can inderstand sequencing
NGS Illumina. Really changed the field.
How we determine that is the primer to an unknown sequence?
You are a fantastic aide. I'm working with a virus not bacteria.looking up dsdna group 2 there are many vectors for adenoviruses
There must be a general
Vector used for dsdna .
Profffffff😭😭😭 thankyouuuu
Someone give this guy a Nobel Prize please!
14:50 OK so how to make such a 2',3'-di-deoxy nucleotide in a beaker?
Oh, and BTW, where to get/synthesize the normal 3'-hydroxyl bases anyway?
And what are those colourful fluorescent dyes to attach to them?
Bon Bon variable number of tandem repeat probes. These probes are radioactives. Idk why after 3 yrs but I still felt like sharing
How do you purify the plasmid though?