Bacterial Transformation

For more information, visit www.bio-rad.com/yt/bacterial-t....
This video demonstrates how to perform a bacterial transformation using the Bio-Rad pGLO™ bacterial transformation kit. The CaCl2 method is used to transform E. coli HB101 bacteria. Proper aseptic technique, heat-shock method, and sample labeling are shown.
www.bio-rad.com/evportal/desti...
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[@nataliemacias1699]

we have to do labs like this because of covid

[@VitorPereira-xy6bx]

ik :/

[@michaela9499]

girl same!

[@gxttersnipe1482]

low key upset i can't be doing a real lab

[@zaworudo3918]

lol and you can't even ask the professor questions. Just got to wait for them to respond to your messages a week late.

[@micahzile9430]

big sad

[@uberawsome3696]

As an amateur this is very useful

[@DBS608]

Is this procedure works with this strain and/or the same plasmid only? Or this procedure can also works woth other strains and/or other plasmid too?

[@croberto7]

UofT BIO230 gang

[@Nicole72591]

still loving the coloring book!

[@mattm345]

I’m a student worker and I’m trying to isolate the plasmid itself and we can’t seem to do it, I’m trying to find examples on line to see what part of it were messing up

[@rubinaaltaf999]

Great video!

[@reiani8283]

why does he low key sound like ethan from h3h3 lmao

[@zaworudo3918]

Man I'm going to fail this class.

[@chavfamilia200]

Same

[@vishnupullela3146]

😂

[@guacardo491]

maybe me too

[@Pablo-on7xm]

Just did it today it was easy, I'm in 7th grade💀

[@charan.ravuri7624]

Same situation

[@brookeholmes9223]

His glove is too big

[@thekyloution3515]

Harrison Riggs was here

[@vardhanbivalkar822]

Does this plasmid give the bacteria the green fluorescent gene making them glow?

[@mahdighasemi9294]

im pretty sure because pGLO is the plasmid that glows under UV light.

[@connorribera4198]

Yes

[@connorribera4198]

It contains the protein and the antibiotic resistance

[@cleo7663]

does anyone know why the petri dish should be placed upside down in the last step?

[@mriaz7289]

probably to avoid condensation from dripping onto the bacteria?

[@cleo7663]

@@mriaz7289 oh that makes so much sense. Thank you!

[@mriaz7289]

@@cleo7663 welcome!

[@Sohodollie]

Is the process the same for yeast?

[@Sohodollie]

Besides different growth media

[@user-rr1ts7cq7t]

I don't understand English. Im speake Arabic

[@mbangisenimuelelwa5880]

Wat is Lb stand for in plate

[@seabasszamarripa8252]

Luria Broth. It’s a type of nutrient that helps the bacteria grow!

[@Born2beasavior17]

No results!!

[@cutepapaya4886]

Clarkson Bio II Lab Gang

[@jeremyprzygoda115]

Shoutout to everybody in Mr. Croteau's DNA Science class watching this

[@kellyijang3542]

I hope it was just a display error and not a lab error at 03:31 when they said "transfer 250 microliters" but showed 520 microliters.

[@DBS608]

Its 250 ul only.

[@_mustafaS]

NCSU gang

[@kaelinsherman7232]

Mrs. Zee bio gang

[@davidfrancis2139]

Who is here from Philip's assignment??
RRC!!!!

[@user-rr1ts7cq7t]

lm speak Arabic