Coagulase Test

In this video, Biology Professor (Twitter: @DrWhitneyHolden) will cover the Coagulase test procedure and discuss the background information to understand how this test works. Specifically, this diagnostic test indicates the presence of the enzyme coagulase in a bacterial culture. This is an enzyme that acts as a virulence factor, helping to disguise the bacterium as a blood clot so it might hide from phagocytes. This test is used to differentiate coagulase-positive bacteria, like S. aureus, from coagulase-negative staphylococci. The two versions of this test, the slide test and tube test, are described.
Want a PDF of the final white board with all the answers written in? Download FREE STUDY NOTES here: tinyurl.com/Bi...

[@TheCR33P3RZ]

Thank you for all these amazing videos, theyve been super helpful. Would you consider making a video about the Optochin test? Thanks again!

[@olenak3112]

You are naturally born teacher! Thank you

[@PG-ck5pw]

thank you, it was very well explained

[@akankshakadukar2834]

Very nice explanation ma'am😇❣️🙏🏻.... Thank you 🤗

[@kunwarriderr]

You are teaching veryyyy welll😍😍😍😍

[@fidhanihal2812]

Why do we have two separate tests for the two forms of the enzyme?Why can’t we detect bound coagulase in the tube test OR free form in the slide test?

[@abdurraheemkhan2791]

Thank you 😊
can I get the second video

[@BiologyProfessor]

I have 100+ videos for you.

[@saikrishnavissa4902]

Awesome explanation 🙏

[@subhranilmanna9715]

mam, i would like to watch lecture videos on sucrose and nitrogen biochemical test........
your videos are really great to learn..........

[@ahmedobaid5699]

Thank you for this vedio
Why coagulase converts fibrnogin to fibrin

[@BiologyProfessor]

It results in blood clotting. The resulting clot protects the bacterium from phagocytosis (we think).

[@gamalmohamedashraf2746]

Thank you for your effort,
but I have a question, Why not to use the blood instead of the plasma???

[@BiologyProfessor]

The proteins that clot in the presence of coagulase are more concentrated in the plasma.

[@VickyRajput-ds2kw]

Thank you mam..

[@sachinmanoj9278]

Excellent 👌

[@samahahmed1673]

Thank you so much

[@BiologyProfessor]

You're most welcome

[@gufranpagnaaabdul-rauf4226]

That was very helpful

[@ingridbudrovich1211]

Thank you for all of these wonderful MicroBio test videos! I was wondering if you have a recommendation for information on the Bile-Esculin test? I did not see a video of yours for this test (which I prefer), but if you have a source you recommend for other microbio tests, I would love to hear it!

[@BiologyProfessor]

I will add it to my list - thank you! Here is a website with a great summary: microbiologyinfo.com/bile-esculin-test/

[@ayanbag174]

Thank you mam this video is easy to understand 🙏

[@a_a8364]

Thank you very much 😍😍❤️

[@BiologyProfessor]

You are welcome!

[@spirulinachlorella1629]

hi!!!! you know a good shop to get testtubes for differentiation??? cant find any here!!!!

[@ivanduong7329]

Thank you for this wonderful knowledge, Professor. May I have a question, please? Why do we check for the coagulase tube test after 90 minutes, instead of leaving it overnight? Is there any change if that was a positive test and we leave it too long?
Thank you!

[@BiologyProfessor]

You might get a false positive if you leave it too long.

[@ivanduong7329]

@@BiologyProfessor Does the clump get melted? If yes, what factor has made it melt?
Thank you very much, Professor

[@BiologyProfessor]

No, there is no melting. I the clump could be disrupted by vigorous shaking, but I’ve never tried.

[@ivanduong7329]

@@BiologyProfessor Thank you so much Professor. This helps me a lot.

[@Rannbo]

Very helpful. Appreciate the video Professor.

[@BiologyProfessor]

You are welcome. Share with your classmates 😉

[@satishchandra7448]

Pls provide list of coagulase positive bacteria.

[@BiologyProfessor]

From Wikipedia: List of coagulase-positive staphylococci: Staphylococcus aureus subsp. anaerobius, S. aureus subsp. aureus, S. delphini, S. hyicus, S. intermedius, S. lutrae, and Staphylococcus schleiferi subsp. coagulans.

[@AmjadAli-fy3gx]

Make vido on lasmeniases deases

[@BiologyProfessor]

Leishmaniasis? I will add it to my list for future videos. Thanks for the idea!

[@Shyb_zkh001]

Thank u

[@nitinsinghbist1725]

Amazing ❤️.

[@amnazulfiqar8427]

Appreciated👏😍

[@BiologyProfessor]

🤗 You are welcome

[@SauravKumar-xf6tn]

Upload new videos of microbiology

[@SauravKumar-xf6tn]

Your videos are very easy to understand

[@bhumikachetwani4374]

Finally understood 🥂🙏thanku so much

[@bahahos7426]

Thank uuu

[@mayanksangam9205]

Thank you professor :)

[@BiologyProfessor]

Thank you for watching. If my videos are helping you learn, please share them with others. :)

[@mayanksangam9205]

@@BiologyProfessor Ofcourse professor :)

[@adilfarooq5582]

Nice

[@akKhan-hs9sj]

can we use human blood instead of rabbit

[@BiologyProfessor]

Yes www.researchgate.net/post/Can_I_use_human_plasma_for_coagulase_testing

[@samiranscreation3364]

wow I am learning from Emma Watson

[@saadahyounis]

May Allah bless you, thanks

[@themonkeyminds7252]

Great! Keep it up!
Peace:)

[@themonkeyminds7252]

@Rayden Maxton thanks

[@masroorkhalil9655]

how you lossses your weight

[@BiologyProfessor]

a ketogenic diet - it is wonderful!

[@masroorkhalil9655]

Biology Professor
what is this ketogenic diet

[@rasheed.hmusic256]

I love you. awesome vid

[@kshitijgiripunje7253]

Mam u looking different
How did u lose ur weight

[@BiologyProfessor]

Keto

[@jamesnollase891]

thank you!!